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Showing: 1 - 50 of 244 results

  20. PB-NES-ZapCV2

    Plasmid
    #222950
    Purpose
    Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV2 (NES Synthetic)
    Use
    Tags
    ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222950-plasmid-map-sequence-id-450583

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  34. pLaG2-T7RNAPC

    Plasmid
    #216337
    Purpose
    Arabinose-inducible PBad promoter driving expression of the anti-eGFP nanobody LaG2 translationally fused to the C-terminal portion (residues 180-883) of T7RNAP
    Depositor
    Mark Styczynski
    Insert
    LaG2-T7RNAPC fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    g2658a, g3915a
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    216337-plasmid-map-sequence-id-456751

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  37. pSNAPC5-donor

    Plasmid
    #176341
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White, Barbara Stranger
    Insert
    SNAPC5 (SNAPC5 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    176341-plasmid-map-sequence-id-347185

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  38. APC041-G05

    Plasmid
    #58759
    Purpose
    bacterial expression of PDB 4O45
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4O45 (WDR5 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available since
    Aug. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  39. pcDNA-mito-ZapCY1

    Plasmid
    #58996
    Purpose
    Mitochondrial Zn-binding FRET biosensor
    Depositor
    Amy Palmer
    Article
    Park et al ACS Chem Biol. 2012 Aug 10.
    Insert
    mito-ZapCY1 high affinity Zn(II) sensor
    Use
    Tags
    See comment
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  49. pcDNA-NES-ZapCY2

    Plasmid
    #59016
    Purpose
    Cytosolic zinc-binding FRET biosensor
    Depositor
    Amy Palmer
    Article
    Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71
    Insert
    NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
    Use
    Tags
    Nuclear Exclusion Signal
    Expression
    Mammalian
    Mutation
    See comment
    Promoter
    CMV
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 50 of 244 results