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We narrowed to 255 results for: petD

Showing: 1 - 50 of 255 results

  2. pETDest

    Plasmid
    #124245
    Purpose
    Allows Gateway cloning into the bacterial 6xHIS tagged protein expression vector pET26
    Depositor
    Attila Fehér
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    124245-plasmid-map-sequence-id-257540

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  4. pETDuet_huCAD_ICADL (TevS)

    Plasmid
    #100098
    Purpose
    dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences
    Depositor
    Jim Wells
    Article
    Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112
    Insert
    DNA fragmentation factor subunit alpha (CAD, DFFA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    two caspase cleavage sites (DETD-117 and DAVD-224…
    Promoter
    T7
    Available since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    100098-plasmid-map-sequence-id-261413

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  7. pETDuet-1 TIM9,10

    Plasmid
    #170280
    Purpose
    Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production
    Depositor
    Paul Schanda
    Article
    Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25.
    Insert
    mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…
    Available since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170280-plasmid-map-sequence-id-334954

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  9. pETDuet-TFIIF

    Plasmid
    #171082
    Purpose
    Co-expresses human TFIIF. The resulting plasmid was used to generate a expression construct encoding 2 subunits, with His-tag on RAP30. Originally from MN Gonzalez, was submitted with permission
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    RAP30 (GTF2F2 Human)
    RAP74 (GTF2F1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171082-plasmid-map-sequence-id-335551

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  13. pETDuet-TFIIE

    Plasmid
    #171083
    Purpose
    Co-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permission
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    TF2E1 (GTF2E1 Human)
    TF2E2 (GTF2E2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171083-plasmid-map-sequence-id-335640

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  14. pETDuet-1 TIM8,13

    Plasmid
    #170282
    Purpose
    Expresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production
    Depositor
    Paul Schanda
    Article
    Sucec et al Sci Adv. 2020 Dec 18;6(51). pii: 6/51/ea
    Insert
    yeast Tim8 and Tim13 (TIM8 Budding Yeast)
    Use
    Tags
    TEV-cleavable N-terminal His tag on Tim13 (on bac…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170282-plasmid-map-sequence-id-334956

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  15. pME_Cp_0_5_017_petD_3'UTR_Cr

    Plasmid
    #235915
    Purpose
    petD 3'UTR Chlamydomonas reinhardtii
    Depositor
    Tobias Erb
    Insert
    petD 3'UTR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    235915-plasmid-map-sequence-id-471494

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  22. pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher

    Plasmid
    #117656
    Purpose
    Bacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.
    Depositor
    Bo Huang
    Article
    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.
    Inserts
    sfCherry2(1-10)
    sfCherry2(11)-SpyCatcher002
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    117656-plasmid-map-sequence-id-226764

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  24. pETDuet-msGC-NT21

    Plasmid
    #78102
    Purpose
    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta
    Depositor
    William Montfort
    Article
    Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.
    Inserts
    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    fragment 1-380 of the beta subunit and fragment 2…
    Promoter
    T7
    Available since
    June 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    78102-plasmid-map-sequence-id-151428

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  25. pETDuet-msGC-NT13

    Plasmid
    #75087
    Purpose
    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta
    Depositor
    William Montfort
    Article
    Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.
    Inserts
    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    fragment 1-380 of the beta subunit and fragment 4…
    Promoter
    Available since
    May 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    75087-plasmid-map-sequence-id-148282

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  27. pETDuet-MerTK_KRmutant-PTPN1

    Plasmid
    #196447
    Purpose
    Expression of MerTK Kinase Domain in E. coli and surface entropy reduction mutant for crystallization, coexpress PTPN1
    Depositor
    Byung Il Lee
    Article
    Bae et al Exp Mol Med. 2022 Sep;54(9):1450-1460. d
    Inserts
    Tyrosine-Protein Kinase Mer (MERTK Human)
    PTPN1 (PTPN1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    K591R, K639R, K702R, K865R
    Promoter
    T7 promoter
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    196447-plasmid-map-sequence-id-388271

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  29. pETDuet-msGC-NT2

    Plasmid
    #74763
    Purpose
    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta
    Depositor
    William Montfort
    Article
    Hu et al J Biol Chem. 2008 Jul 25;283(30):20968-7
    Inserts
    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    fragment 49-471 of the alfa subunit
    Promoter
    T7
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    74763-plasmid-map-sequence-id-141369

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  37. pETDuet-1-6xHis-TEV-Nsp8

    Plasmid
    #201022
    Purpose
    Bacterial expression of codon optimized SARS-CoV-2 nsp8 tagged with an N-terminus His-tag followed by a TEV protease cleavage site.
    Depositor
    Sharon Rozovsky
    Article
    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d
    Insert
    non-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    201022-plasmid-map-sequence-id-394460

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Showing: 1 - 50 of 255 results