Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 5,597 results for: codon optimized

Clear keywords
Showing: 1 - 20 of 5597 results

  2. Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

    Plasmid
    #46168
    Purpose
    Codon optimized Cas9_SV40 NLS with intron
    Depositor
    John Calarco
    Publication
    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    codon optimized Cas9_SV40 NLS with intron
    Use
    CRISPR
    Mutation
    intron inserted at position 3113-3163, and SV40 N…
    Promoter
    eef-1A.1 (eft-3)
    Available since
    July 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46168-plasmid-map-sequence-id-65793

    Enlarge

  4. Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

    Plasmid
    #46168
    Purpose
    Codon optimized Cas9_SV40 NLS with intron
    Depositor
    John Calarco
    Publication
    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    codon optimized Cas9_SV40 NLS with intron
    Use
    CRISPR
    Mutation
    intron inserted at position 3113-3163, and SV40 N…
    Promoter
    eef-1A.1 (eft-3)
    Available since
    July 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46168-plasmid-map-sequence-id-65793

    Enlarge

  5. pHsp70-Cas9

    Plasmid
    #45945
    Purpose
    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.
    Depositor
    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger
    Publication
    Gratz et al Genetics. 2013 May 24.
    Insert
    Codon optimized Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Mutation
    Silent change from C to A at bp 834 of insert
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    45945-plasmid-map-sequence-id-116251

    Enlarge

  6. pBS-Hsp70-Cas9

    Plasmid
    #46294
    Purpose
    A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.
    Depositor
    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger
    Publication
    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1
    Insert
    codon optimized Cas9
    Use
    CRISPR
    Tags
    3x Flag
    Expression
    Insect
    Mutation
    Silent change from C to A at bp 834 of insert
    Promoter
    Hsp70
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46294-plasmid-map-sequence-id-332831

    Enlarge

  8. pCRISPomyces-2

    Plasmid
    #61737
    Purpose
    Streptomyces expression of codon-optimized Cas9 and custom gRNA
    Depositor
    Huimin Zhao
    Publication
    Cobb et al ACS Synth Biol. 2014 Dec 8.
    Inserts
    sSpCas9
    gRNA cassette
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    BbsI-flanked lacZ cassette inserted in place of s…
    Promoter
    gapdhp(EL) and rpsL(XC)-BbsI
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61737-plasmid-map-sequence-id-190162

    Enlarge

  9. pCRISPR-aBEST

    Plasmid
    #131464
    Purpose
    Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes
    Depositor
    Tilmann Weber
    Publication
    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Streptomyces codon optimized spCas9n (D10A)
    streptomyces codon optimized tadA
    Use
    CRISPR and Synthetic Biology
    Promoter
    tipA
    Available since
    Nov. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131464-plasmid-map-sequence-id-261686

    Enlarge

  10. JDS246

    Plasmid
    #43861
    Purpose
    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters
    Depositor
    Keith Joung
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    Use
    CRISPR
    Tags
    3X FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    43861-plasmid-map-sequence-id-59200

    Enlarge

  11. pBS-Hsp70-Cas9

    Plasmid
    #46294
    Purpose
    A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.
    Depositor
    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger
    Publication
    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1
    Insert
    codon optimized Cas9
    Use
    CRISPR
    Tags
    3x Flag
    Expression
    Insect
    Mutation
    Silent change from C to A at bp 834 of insert
    Promoter
    Hsp70
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46294-plasmid-map-sequence-id-332831

    Enlarge

  12. pHsp70-Cas9

    Plasmid
    #45945
    Purpose
    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.
    Depositor
    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger
    Publication
    Gratz et al Genetics. 2013 May 24.
    Insert
    Codon optimized Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Mutation
    Silent change from C to A at bp 834 of insert
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    45945-plasmid-map-sequence-id-116251

    Enlarge

  14. pCRISPomyces-2

    Plasmid
    #61737
    Purpose
    Streptomyces expression of codon-optimized Cas9 and custom gRNA
    Depositor
    Huimin Zhao
    Publication
    Cobb et al ACS Synth Biol. 2014 Dec 8.
    Inserts
    sSpCas9
    gRNA cassette
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    BbsI-flanked lacZ cassette inserted in place of s…
    Promoter
    gapdhp(EL) and rpsL(XC)-BbsI
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61737-plasmid-map-sequence-id-190162

    Enlarge

  15. pEF1a-hMLH1dn

    Plasmid
    #174824
    Purpose
    Mammalian expression of human MLH1dn (codon optimized)
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human MLH1dn (codon optimized)
    Expression
    Mammalian
    Mutation
    deletion of residues 754-756
    Promoter
    EF1a
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    174824-plasmid-map-sequence-id-343630

    Enlarge

  16. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid
    #107941
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R)
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R and K548R
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107941-plasmid-map-sequence-id-418295

    Enlarge

  17. pMLM3705

    Plasmid
    #47754
    Purpose
    Expresses mammalian cell codon-optimized dCas9-VP64
    Depositor
    Keith Joung
    Publication
    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    codon optimized dCas9-VP64
    Use
    CRISPR
    Tags
    3x FLAG, NLS, and VP64
    Expression
    Mammalian
    Mutation
    D10A and H840A mutations in Cas9 (catalytically i…
    Promoter
    CMV
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47754-plasmid-map-sequence-id-68222

    Enlarge

  18. phCMV-coGALVenv-C4070A

    Plasmid
    #172217
    Purpose
    Expresses the envelope protein of Gibbon Ape Leukemia Virus (GALV) for pseudotyping of retroviral or lentiviral vectors
    Depositor
    Boris Fehse
    Publication
    Mirow et al Viruses. 2021 Jul 27;13(8). pii: v130814
    Insert
    Envelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)
    Expression
    Mammalian
    Mutation
    Carries C-terminus of amphotropic MLV strain 4070…
    Promoter
    CMV immediate early promoter
    Available since
    Aug. 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    172217-plasmid-map-sequence-id-341502

    Enlarge

  20. AAV-pgk-Cre

    Plasmid
    #24593
    Depositor
    Patrick Aebischer
    Has service
    AAV Retrograde
    Insert
    Cre recombinase (codon optimized)
    Use
    AAV and Cre/Lox; Adeno-associated
    Promoter
    pgk
    Available since
    May 26, 2010
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    24593-plasmid-map-sequence-id-197583

    Enlarge

Showing: 1 - 20 of 5597 results