Showing: 181 - 200 of 7975 results
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Plasmid#131236PurposeMammalian expression of S497A DNA polymerase iota with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains a point mutation converting Pol iota ser…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_S520A POLI
Plasmid#131237PurposeMammalian expression of S520A DNA polymerase iota with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains a point mutation converting Pol iota ser…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_S580A POLI
Plasmid#131238PurposeMammalian expression of S580A DNA polymerase iota with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains a point mutation converting Pol iota ser…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_3KA POLI
Plasmid#131239PurposeMammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A and K446ADepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains point mutations that convert lysine resi…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pOpen-K12polLF (exo-)
Plasmid#165522PurposeDNA pol fragment used in flourescent labelling for microarray, dA and dT tailing, and ligating DNA adapters to DNA fragments.DepositorInsertDNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-Tlipol (exo-)
Plasmid#165523PurposeDNA polymerase; lacks 3' to 5' exonuclease activity.DepositorInsertTli DNA polymerase (exo-)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-GBDpol (exo)
Plasmid#165572PurposeRobust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEBDepositorInsertPyrococcus Sp. Heat-Stable (exo-) DNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pMS2M-PH-gagpol-D64V
Plasmid#166031PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertMS2-Gag-Pol
UseLentiviralTagsExpressionMutationD64V mutation within the integrase within PolPromoterAvailabilityAcademic Institutions and Nonprofits only -
GLN, Linear-3, PolyU(1)
Plasmid#166984PurposeTests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.DepositorInsertTermination module:PolyU(1)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
GLN, Linear-3, PolyU(4)
Plasmid#166985PurposeTests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.DepositorInsertTermination module:PolyU(4)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
GLY, Linear-3, PolyU(1)
Plasmid#166988PurposeTests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLNYtRNA promoter.DepositorInsertTermination module:PolyU(1)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
GLY, Linear-3, PolyU(4)
Plasmid#166989PurposeTests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.DepositorInsertTermination module:PolyU(4)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-1, PolyU(0)
Plasmid#166992PurposeTests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination module:PolyU(0)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27Linear-2, PolyU(0)
Plasmid#166994PurposeTests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination module:PolyU(0)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7 RNA POL
Plasmid#138352PurposeExpressing T7 RNA Polymerase in mammalian cellsDepositorInsertT7 RNA POL
UseCRISPR and LentiviralTagsNoExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pUC19-POLBHR-eGFP
Plasmid#176064PurposeHomology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1DepositorInsertEGFP-PolB (POLB Human)
UseTagsEGFPExpressionMammalianMutationpUC with endogenous SapI mutated out, PAM mutatio…PromoterN/AAvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgPole#1/Cre
Plasmid#173609PurposeExpresses a Pole-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Pole (Pole Mouse)
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgPole#2/Cre
Plasmid#173610PurposeExpresses a Pole-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Pole (Pole Mouse)
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-PolB(WT)
Plasmid#177131PurposeBacterial vector for expression of an N-terminal GST fusion of PolB(WT) with a TEV protease site located between the GST tag and PolB(WT)DepositorInsertPolB (POLB Human)
UseTagsGSTExpressionBacterialMutationPromoterTacAvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide-PolB1-puro
Plasmid#177146PurposeLentiviral vector expressing PolB-gRNA-1 and a puromycin resistance cassetteDepositorInsertPolBgRNA1 (POLB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only
Showing: 181 - 200 of 7975 results