We narrowed to 15,064 results for: pET
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Plasmid#128844Purposebacterial expression of pR-spondin 1DepositorAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only
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N8his-GFPenhancer-GGGGS4-LaG16
Plasmid#140442PurposeWe designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.DepositorInsertGFPenhancer-GGGGS4-LaG16
UseTags8 histidine tagExpressionBacterialMutationPromoterT7 promotorAvailable SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pMSP1E3D1_D73C
Plasmid#39328DepositorInsertMSP1E3D1_D73C (APOA1 synthetic gene)
UseTags6xHisExpressionBacterialMutationcys mutant of MSP1E3D1PromoterT7Available SinceSept. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pScPPX2
Plasmid#112877PurposeExpresses His-tagged yeast exopolyphosphatase for purification from E. coliDepositorAvailable SinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT
Plasmid#26106PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTags6xHis, TEV cleavage site, and TrxAExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available SinceDec. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-Nedd4L
Plasmid#186806PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-Nedd4L.DepositorAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Alpha-Synuclein-His
Plasmid#186802PurposeCodon-optimized bacterial expression plasmid. Expresses Alpha-Synuclein-His.DepositorAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
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PVS10
Plasmid#104398Purposepolycistronic vector for expression of E. coli core RNAPDepositorUseTagsHis6ExpressionBacterialMutationPromoterT7Available SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSP1E2D1
Plasmid#161606PurposeBacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formationDepositorInsertMSP1E2D1
UseTags7-HisExpressionBacterialMutation"extended" MSP1D1; contains repeat of h…PromoterT7Available SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Twin-Strep(R)-Tev-GSGS-Ubiquitin
Plasmid#186803PurposeCodon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.DepositorInsertTwin-Strep(R)-Tev-GSGS-Ubiquitin
UseTagsGS-Strep (WSHPQFER)-GGGSGGGSGGSSA-Strep (WSHPQFER…ExpressionBacterialMutationStrep tag Lysine's mutated to ArgininePromoterAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ841
Plasmid#39318DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationAspartate 10 to Alanine (D10A) and Histidine 840 …PromoterT7Available SinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMSP1E1D1
Plasmid#161605PurposeBacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formationDepositorInsertMSP1E1D1
UseTags7-HisExpressionBacterialMutation"extended" MSP1D1; contains repeat of h…PromoterT7Available SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLS.491
Plasmid#184956PurposeTest effect of a1/a2 length on editing rpoB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12
UseTagsExpressionBacterialMutationrpoB donor T1534CPromoterT7/lacAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
TrpLE-M-nMSP1E3D1
Plasmid#179949PurposeTagless membrane scaffold protein (MSP) for formation of nanodiscs.DepositorInsertTrpLE-M-nMSP1E3D1
UseTagsTrpLEExpressionBacterialMutationNative Trp residues have been mutated to Phe resi…PromoterT7Available SinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSP2
Plasmid#26282DepositorInsertMSP2
UseTags6-HisExpressionBacterialMutationsynthetic gene of dimer of deletion mutant (1-43)…PromoterAvailable SinceApril 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp
Plasmid#183069PurposeBacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.DepositorInsertCas12c_4
UseCRISPRTagsHis10 and MBPExpressionBacterialMutationWild-typePromoterAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
CL7-LbCas12aD156R
Plasmid#164659PurposeBacterial expression of mutated LbCas12aD156R using CL7/Im7DepositorInsertLbCas12a-D156R
UseTagsThioredoxin, CL7, SUMOExpressionBacterialMutationchanged Aspartic Acid 156 to ArgininePromoterT7Available SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-NKX3.1
Plasmid#42896DepositorAvailable SinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSC-SmBiT
Plasmid#207632PurposeA plasmid encoding photocaged SpyCatcher (pSC) fused to SmBiTDepositorInsertphotocaged SpyCatcher-SmBiT
UseTags6xHisExpressionBacterialMutationAmber stop codon at SC's critical lysinePromoterT7Available SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Danio rerio cryabb
Plasmid#47665PurposeRecombinant protein productionDepositorAvailable SinceSept. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZF-BCB-PDZ
Plasmid#127478Purposecoding sequence for synthetic zinc finger ZF-BCB-PDZDepositorInsertZF-BCB synthetic transcription factor fused with the alpha 1-syntrophin PDZ domain
UseCell-free transcription–translationTagsalpha 1-syntrophin PDZ domainExpressionMutationPromoterT7Available SinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZF-ADD-L
Plasmid#127477Purposecoding sequence for synthetic zinc finger ZF-ADD-LDepositorInsertZF-ADD synthetic transcription factor fused with PDZ cognate peptide ligand (VKESLV)
UseCell-free transcription–translationTagsPDZ cognate peptide ligand (VKESLV)ExpressionMutationPromoterT7Available SinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-A OR (S AND T)-SpyTag
Plasmid#243781PurposeExpresses multifunctional construct enabling the OR(AND)-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR (sortase eSrtA(4S9) AND TEV) treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
UseTags6x His tagExpressionBacterialMutationPromoterAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-S OR (A AND T)-SpyTag
Plasmid#243782PurposeExpresses multifunctional construct enabling the OR(AND)-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) OR (sortase eSrtA(2A9) AND TEV) treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
UseTags6x His tagExpressionBacterialMutationPromoterAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-T OR (A AND S)-SpyTag
Plasmid#243783PurposeExpresses multifunctional construct enabling the OR(AND)-gated release of mGreenLantern from materials in response to TEV OR (sortase eSrtA(2A9) AND sortase eSrtA(4S9) ) treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
UseTags6x His tagExpressionBacterialMutationPromoterAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-A OR S OR T-SpyTag
Plasmid#243784PurposeExpresses multifunctional construct enabling the OR OR-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR sortase eSrtA(4S9) OR TEV treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
UseTags6x His tagExpressionBacterialMutationPromoterAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-A AND S AND T-SpyTag
Plasmid#243785PurposeExpresses multifunctional construct enabling the AND AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND sortase eSrtA(4S9) AND TEV treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
UseTags6x His tagExpressionBacterialMutationPromoterAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-S AND T-SpyTag
Plasmid#243788PurposeExpresses multifunctional construct enabling the AND-gated release of mCerulean from materials in response to sortase eSrtA(4S9) AND TEV treatment. Contains a SpyTag for material tethering.DepositorInsertmCerulean
UseTags6x His tag and SsrAExpressionBacterialMutationPromoterAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
HER2Nb-C AND (A OR T)-SpyTag
Plasmid#243790PurposeExpresses multifunctional construct enabling the AND(OR)-gated release of a HER2 Nanobody from materials in response to HRV-3C AND (sortase eSrtA(2A9) OR TEV) treatment.DepositorInsertHER2 nanobody
UseTags6x His tag and SsrAExpressionBacterialMutationPromoterAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-A AND S-SpyTag
Plasmid#243775PurposeExpresses multifunctional construct enabling the AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND sortase eSrtA(4S9) treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
UseTags6x His tag and SsrAExpressionBacterialMutationPromoterAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-S AND T-SpyTag
Plasmid#243777PurposeExpresses multifunctional construct enabling the AND-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) AND TEV treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
UseTags6x His tag and SsrAExpressionBacterialMutationPromoterAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern- A AND (S OR T)-SpyTag
Plasmid#243778PurposeExpresses multifunctional construct enabling the AND(OR)-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND (sortase eSrtA(4S9) OR TEV) treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
UseTags6x His tag and SsrAExpressionBacterialMutationPromoterAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern- S AND (A OR T)-SpyTag
Plasmid#243779PurposeExpresses multifunctional construct enabling the AND(OR)-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) AND (sortase eSrtA(2A9) OR TEV) treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
UseTags6x His tag and SsrAExpressionBacterialMutationPromoterAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern- T AND (A OR S)-SpyTag
Plasmid#243780PurposeExpresses multifunctional construct enabling the AND(OR)-gated release of mGreenLantern from materials in response to TEV AND (sortase eSrtA(2A9) OR sortase eSrtA(4S9)) treatment. Contains a SpyTag for material tethering.DepositorInsertmGreenLantern
UseTags6x His tag and SsrAExpressionBacterialMutationPromoterAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
MBP-Streptavidin-RGG
Plasmid#242451PurposeExpresses the MBP-Streptavidin-RGG fusion protein in E Coli.DepositorInsertMBP-Streptavidin-RGG (using LAF-1 RGG domain)
UseTagsMBP and SteptavidinExpressionBacterialMutationPromoterAvailable SinceSept. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
small ubiquitin-like modifier 3 (SUMO3)
Plasmid#221053PurposeExpression of small ubiquitin-like modifier 3 (SUMO3) protein under T7 promoterDepositorAvailable SinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-P-Domain
Plasmid#201193PurposeExpresses furin P-domain genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-P-Domain
UseTagsHisTag and SpyCatcher001ExpressionBacterialMutationPromoterT7Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM57
Plasmid#227654PurposepETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, V80A)DepositorUseTagsH6-PreScission and PreScission-StrepExpressionBacterialMutation1-179 and amino acids 2-239, V80APromoterT7Available SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM54
Plasmid#227653PurposepETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, L132A)DepositorUseTagsH6-PreScission and PreScission-StrepExpressionBacterialMutation1-179 and amino acids 2-239, L132APromoterT7Available SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM72
Plasmid#227622PurposepETDuet-1 Rpn8(1-179)-precission-Strep, H6-precission-Rpn11(2-239, G77P)DepositorUseTagsHis-PrescissionExpressionBacterialMutation1-179 and aa 2-239, G77PPromoterT7Available SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM71
Plasmid#227621PurposepETDuet-1 H6-precission-Rpn11(24-160-GGSGSG-192-221) , Rpn8(1-179)DepositorUseTagsHis-PrescissionExpressionBacterialMutation1-179 and amino acids 24-160-GGSGSG-192-221PromoterT7Available SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
nUnaG-ST
Plasmid#207630PurposeA plasmid encoding the N-terminal UnaG fragment fused to SpyTag.DepositorInsertnUnaG-SpyTag-MBP
UseTags6xHisExpressionBacterialMutationaa 1-84PromoterT7Available SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-DIIG
Plasmid#201196PurposeExpresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-DIIG
UseTagsHisTagExpressionBacterialMutationPromoterT7Available SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-SWG
Plasmid#201194PurposeExpresses a peptide from the active site of furin genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-SWG
UseTagsHisTagExpressionBacterialMutationPromoterT7Available SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-PEED
Plasmid#201195PurposeExpresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-PEED
UseTagsHisTagExpressionBacterialMutationPromoterT7Available SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT348
Plasmid#204515PurposeBacterial expression of CEC-5 C-terminal fragment with 6xHis and maltose binding protein tags for use as an antigenDepositorInsertC-terminal fragment of cec-5 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Annexin A7-TEV-TwinStrep
Plasmid#198635PurposeAnnexin A7 fibrillization and lipid bindingDepositorAvailable SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only