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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,285 results for: crispr

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Showing: 1981 - 2000 of 25285 results

  1. pX330-EN1201

    Plasmid
    #92144
    Purpose
    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus
    Depositor
    Benoit Bruneau
    Publication
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
    Expression
    Mammalian
    Available since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10 Articles
    92144-plasmid-map-sequence-id-177211

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  2. YE1-BE4max-NG

    Plasmid
    #138159
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Within rAPOBEC1 W90Y + R126E; within Cas9 D10A + …
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    138159-plasmid-map-sequence-id-272341

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  5. pcDNA3.1-hAsCpf1(TYCV) (pY210)

    Plasmid
    #89351
    Purpose
    Expresses humanized AsCpf1 variant that recognizes TYCV PAMs
    Depositor
    Feng Zhang
    Publication
    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cpf1, RR variant
    Use
    CRISPR
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    S542R/K607R
    Promoter
    CMV
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    89351-plasmid-map-sequence-id-178227

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  6. pJCC_164 SpCas9 R1333A/R1335A

    Plasmid
    #179528
    Purpose
    For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Publication
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 R1333A/R1335A
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    changed arginines 1333 and 1335 to alanine
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179528-plasmid-map-sequence-id-351744

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  15. pCMV-eNme2-C-ABE8e

    Plasmid
    #185667
    Purpose
    Expresses eNme2-C-ABE8e in mammalian cells
    Depositor
    David Liu
    Publication
    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ecTadA(8e)-neNme2-C
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…
    Available since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    185667-plasmid-map-sequence-id-471552

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  16. pBBR_Cas9_sp1

    Plasmid
    #154851
    Purpose
    Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome
    Depositor
    John van der Oost
    Publication
    Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Spcas9 gene
    sgRNA scaffold
    Use
    CRISPR
    Tags
    6xHis
    Mutation
    Codon harmonized variant for expression in Rhodob…
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154851-plasmid-map-sequence-id-309592

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Showing: 1981 - 2000 of 25285 results