We narrowed to 15,141 results for: kars;

1. pSAG1::CAS9-U6::sg290860-6

   Plasmid

   #59855

   Purpose

   Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.

   Depositor

   David Sibley

   Article

   Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229

   Insert

   CRISPR sg290860-6
   

   Use

   CRISPR; ; toxoplasma gondii

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXFokI-dCas9

   Plasmid

   #60901

   Purpose

   Dual Expression Vector for FokI-dCas9 and gRNA

   Depositor

   Bruce Conklin

   Article

   Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMM9-MAPKK2-WT

   Plasmid

   #40805

   Depositor

   Natalie Ahn

   Article

   Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

   Insert

   MAP2K2 (MAP2K2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Wild type

   Promoter

   MSV-LTR

   Available since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

4. p3XFLAG-MKL1-~100

   Plasmid

   #27176

   Depositor

   Ron Prywes

   Article

   Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

   Insert

   MKL1 (MRTFA Human)
   

   Use

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Deletion of 100 aa from ATG

   Promoter

   Available since

   Dec. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

5. amilCP chromoprotein promoter-less

   Plasmid

   #118008

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   RBS, amilCP
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-DUET-GST-eIF4G1-His6, eIF4E

   Plasmid

   #37232

   Depositor

   Jon Lorsch

   Article

   Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

   Inserts

   TIF4631 (TIF4631 Budding Yeast)
   CDC33 (CDC33 Budding Yeast)
   

   Use

   Tags

   GST and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

7. PU.1-ets-IRES-mCherry

   Plasmid

   #80141

   Purpose

   retroviral expression of PU.1-ets (competitive inhibitor of PU.1) and mCherry

   Depositor

   Ellen Rothenberg

   Article

   Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

   Insert

   PU.1 (Spi1 Mouse)
   

   Use

   Retroviral

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Mutation

   ets = PU.1 DNA binding domain (amino acids 159-26…

   Promoter

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSMP-Dot1L_2

   Plasmid

   #36340

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   Dot1L (DOT1L Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pY012 (pcDNA3.1-hCMtCpf1)

   Plasmid

   #69984

   Purpose

   Expresses humanized CMtCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hCMtCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX PLCg1(C)-SH2

    Plasmid

    #46469

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    PLCg1(C)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 656-766

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSP2279

    Plasmid

    #70706

    Purpose

    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    D10A and H557A in SaCas9

    Promoter

    T7

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pY017 (pcDNA3.1-hPcCpf1)

    Plasmid

    #69989

    Purpose

    Expresses humanized PcCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hPcCpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac1-OSF-TRIM5alpha(chimpanzee)

    Plasmid

    #79032

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(chimpanzee)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. TetO-FUW-SOX3

    Plasmid

    #61546

    Purpose

    Expresses SOX3 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    SOX3 (SOX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pAFMW-AthHEN1

    Plasmid

    #104980

    Purpose

    Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

    Insert

    Hen1 (HEN1 Mustard Weed)
    

    Use

    Tags

    FLAG Myc

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZ005 (AacC2c1 human codon optimized protein)

    Plasmid

    #70171

    Purpose

    Expresses AacC2c1 human codon optimized protein

    Depositor

    Feng Zhang

    Article

    Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

    Insert

    AacC2c1 protein
    

    Use

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-Flag-hsDicer (E1564A)

    Plasmid

    #41587

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    E1564A

    Promoter

    CMV

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pSMP-MBD3_1

    Plasmid

    #36371

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    MBD3 (MBD3 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

19. Flag-Craf S338A

    Plasmid

    #118335

    Purpose

    Expression of human Craf

    Depositor

    Philip Stork

    Article

    Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

    Insert

    Craf S338A (RAF1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S338A

    Promoter

    CMV

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-lxlplap

    Plasmid

    #45581

    Depositor

    Nirao Shah

    Article

    Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

    Inserts

    Green fluorescent protein
    Placental alkaline phosphatase (ALPP Human)
    

    Use

    Lentiviral

    Tags

    Histone 2B

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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