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We narrowed to 13,155 results for: Mpl;

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pCDH1-CMV-FIP200-V44D/V314D/L324D/R543D/R548D-mCherry-Hygro

Plasmid

#210857

Purpose

Lentiviral expression vector encoding FIP200 mutant-mCherry

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

V44D/V314D/L324D/R543D/R548D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z33

Plasmid

#120050

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with FLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

FLAG

Use

Pcr template

Tags

FLAG

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Sport6 Baf60b

Plasmid

#24723

Depositor

Benoit Bruneau

Article

Lickert et al Nature. 2004 Nov 4. 432(7013):107-12.

Insert

Baf60b (Smarcd2 Mouse)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pEBB-II-I-GFP beta isoform

Plasmid

#22147

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pGEX-4T-3-SH3BP1-CTD

Plasmid

#136649

Purpose

Expression of SH3BP1-CTD-GST fusion protein in E. coli

Depositor

Karl Matter, Maria Balda

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1-CTD (SH3BP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Contains only C-terminal domain

Promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-SH3BP1-NTD

Plasmid

#136648

Purpose

Expression of SH3BP1-NTD-GST fusion protein in E. coli

Depositor

Karl Matter, Maria Balda

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1-NTD (SH3BP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Contains only N-terminal domain

Promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLD-puro-CnVA

Plasmid

#24587

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1 EB1 shRNA #3

Plasmid

#37927

Depositor

Torsten Wittmann

Article

Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

Insert

EB1 shRNA #3 (MAPRE1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z17

Plasmid

#120060

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mEos4b

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mEos4b

Use

Pcr template

Tags

mEos4b

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac-His-Flag-Timeless

Plasmid

#22892

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Timeless (TIMELESS Human)

Use

Tags

Flag and His

Expression

Insect

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pTS104 pHAGE2 CMVtetO2 MCS-3C-22xGS-GFP

Plasmid

#199357

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-EGFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-EPSIN 1

Plasmid

#22225

Depositor

Pietro De Camilli

Article

Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

Insert

Epsin 1 (Epn1 Rat)

Use

Tags

Xpress

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only
pMaCTag-P14

Plasmid

#120025

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeptune2.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeptune2.5

Use

Pcr template

Tags

mNeptune2.5

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDGE8

Plasmid

#79463

Purpose

sgRNA shuttle vector; module 2E

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z15

Plasmid

#120058

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with miRFP670

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

miRFP670

Use

Pcr template

Tags

miRFP670

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBG-TFII-I alpha

Plasmid

#22154

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Use

Tags

GST and His

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pCfB8792

Plasmid

#126910

Purpose

Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

ADE2 gRNA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (29-339)

Plasmid

#136544

Purpose

Expresses ANXA2 chimeras in bacterial cells

Depositor

Laszlo Nyitray

Article

Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

Type

Empty backbone

Use

Tags

ANXA2 (29-339) and His-tag

Expression

Bacterial

Mutation

Promoter

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCS021

Plasmid

#207917

Purpose

Level 0 for gRNA in E2 module (5' CCAT-3' ACTA)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKE013

Plasmid

#207916

Purpose

Level 0 for gRNA in E1 module (5' CTGC-3' CCAT)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pKV016

Plasmid

#64767

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, 3x-HA-GFP to a 3xT7-RFP tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Menendez-Benito et al Proc Natl Acad Sci U S A. 2013 Jan 2;110

Insert

RITE cassette

Use

Rite cassette

Tags

Expression

Mutation

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA1 P186C

Plasmid

#66173

Purpose

Expresses the P186C mutant of Sulfolobus solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 1

Use

Tags

His6 tag

Expression

Bacterial

Mutation

P186C

Promoter

T7 promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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L1_UBE2:mS-Lt-Ck4

Plasmid

#136132

Purpose

L1 in position 4, mark cell outline with mScarletI

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mScarletI-Lti6b_3tNos-35S

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMaCTag-P15

Plasmid

#120026

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with miRFP670

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

miRFP670

Use

Pcr template

Tags

miRFP670

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET16b-Trx C35S

Plasmid

#36947

Depositor

Anna Blom

Article

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Thioredoxin-1 C35S mutation (TXN Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

cys 35 to serine

Promoter

T7

Available since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
Integrin alpha5(1-623)-AHCys

Plasmid

#27301

Depositor

Timothy Springer

Article

Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

Insert

integrin alpha 5 (ITGA5 Human)

Use

Tags

Expression

Mammalian

Mutation

alpha 5(1-623)+GG+TEV+GGEN+AHCys

Promoter

Available since

March 8, 2011

Availability

Academic Institutions and Nonprofits only
pKDR-TetOn.1

Plasmid

#183578

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Tet-On

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDR-TetOn.4

Plasmid

#183579

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Tet-On

Available since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDR-EF1a.4

Plasmid

#183571

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 4

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDR-EF1a.2

Plasmid

#183569

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 2

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-D-TOPO-PAmCherry1_160-236-MCS

Plasmid

#60546

Purpose

Cloning PAmCherry1 C-terminal fragment (RC) for BiFC-PALM

Depositor

Xiaolin Nan

Article

Nickerson et al PLoS One. 2014 Jun 25;9(6):e100589. doi:

Insert

PAmCherry1 C-terminal fragment

Use

Cloning

Tags

Expression

Mutation

amino acids 160-236

Promoter

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-D-TOPO-PAmCherry1_1-159-MCS

Plasmid

#60545

Purpose

Cloning PAmCherry1 N-terminal fragment (RN) for BiFC-PALM

Depositor

Xiaolin Nan

Article

Nickerson et al PLoS One. 2014 Jun 25;9(6):e100589. doi:

Insert

PAmCherry1 N-terminal fragment

Use

Cloning

Tags

Expression

Mutation

amino acids 1-159

Promoter

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
px458_2A_GFP_sgRNA_NEMF

Plasmid

#127126

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA NEMF (NEMF Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1deltaC-2xEGFP

Plasmid

#37828

Depositor

Torsten Wittmann

Article

Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deletion after aa248

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pES.HSV2.3a

Plasmid

#69593

Purpose

TCR a chain construct for generation of gBT-I mice

Depositor

Francis R. Carbone

Article

Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

Insert

TCR alpha chain cDNA from clone HSV2.3 (Tcra Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

H-2Kb

Available since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only
pMaCTag-P24

Plasmid

#120035

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TAP

Use

Pcr template

Tags

TAP

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-P10

Plasmid

#120021

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKOk

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKOk

Use

Pcr template

Tags

mKOk

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-NPM NLS2D

Plasmid

#13285

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of aa150-aa155

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pTYF-2xSYN-tTA

Plasmid

#19982

Depositor

Sergey Kasparov

Article

Liu et al J Gene Med. 2008 May . 10(5):583-92.

Insert

2xSYN-tTA (SYN1 Budding Yeast, Human, E.coli)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pSuper-Rem2-2

Plasmid

#51595

Purpose

shRNA 2 against rodent Rem2

Depositor

Suzanne Paradis

Article

Moore et al PLoS One. 2013 Aug 26;8(8):e74751. doi:

Insert

Rem2 (Rem2, Rem2 Mouse, Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

U6

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

Showing: 4001 - 4040 of 13155 results

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