We narrowed to 10,167 results for: otos

1. AAVS1 intron 1 gRNA 27

   Plasmid

   #132396

   Purpose

   Targets AAVS1 intron 1, gRNA: ACCCCACAGTGGGGCCACTA, MLM3636 backbone

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   gRNA targeting AAVS1 intron 1 (PPP1R12C Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mChF-AIP (TD)

   Plasmid

   #61529

   Purpose

   Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TD mutation)

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

   Insert

   FK506 binding protein 1A (FKBP1A Human)
   

   Use

   Tags

   AIP with TD mutation (see comments) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

3. mChF-AIP (TE)

   Plasmid

   #61530

   Purpose

   Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TE mutation)

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

   Insert

   FK506 binding protein 1A (FKBP1A Human)
   

   Use

   Tags

   AIP with TE mutation (see comments) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pLKO.1 mTagBFP2 rat DJ-1 shRNA

   Plasmid

   #222870

   Purpose

   Expresses mTagBFP2 along with an shRNA against rat DJ-1

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   DJ-1 shRNA (Park7 Rat)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAVS1 intron 1 gRNA s3

   Plasmid

   #132395

   Purpose

   Targets AAVS1 intron 1, gRNA: GGGGCCACTAGGGACAGGAT, MLM3636 backbone

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   gRNA targeting AAVS1 intron 1 (PPP1R12C Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJet-CMV-CD52-bglpA

   Plasmid

   #89704

   Purpose

   Human CD52 expression plasmid with short polyA

   Depositor

   Dmitriy Mazurov

   Article

   Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

   Insert

   CMV-CD52-bglpA (CD52 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. YF-AIP

   Plasmid

   #61525

   Purpose

   Expression of human FK506 binding protein 1A (FKBP1A) tagged with EYFP and AIP

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

   Insert

   FK506 binding protein 1A (FKBP1A Human)
   

   Use

   Tags

   AIP and EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

8. CF-AIP

   Plasmid

   #61526

   Purpose

   Expression of human FK506 binding protein 1A (FKBP1A) tagged with ECFP and AIP

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

   Insert

   FK506 binding protein 1A (FKBP1A Human)
   

   Use

   Tags

   AIP and ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

9. Human SLC KO Library

   Pooled Library

   #132552

   Purpose

   Expresses gRNAs for knockout of the human Solute Carrier family

   Depositor

   Giulio Superti-Furga

   Article

   Girardi et al Nat Chem Biol. 2020 Apr;16(4):469-478. d

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

10. pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro

    Plasmid

    #89991

    Purpose

    Donor template for generation of SOX2-ntdTomato reporter cell lines

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

    Insert

    SOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP-imp α

    Plasmid

    #119718

    Purpose

    Expresses EGFP-tagged human importinα in mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

    Insert

    importinα (KPNA2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains amino acids 251-529

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDF-Mm2

    Plasmid

    #197100

    Purpose

    To express the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA and allow UAG codon to be translated into these derivati

    Depositor

    Kensaku Sakamoto

    Article

    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

    Insert

    PylRS variant, Pyl tRNA, kanamycin resistance gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lys61, Glu131, Ala306, Phe384, Glu444 (PylRS)

    Promoter

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTriEx-mCherry-PA-Rac1-T17N

    Plasmid

    #22029

    Purpose

    Expression of photoactivatable Rac1 (dominant-negative mutant).

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
    

    Use

    Tags

    6xHis and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTriEx-mCerulean-PA-Rac1

    Plasmid

    #22030

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    

    Use

    Tags

    6X His and mCerulean

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mCh-KIF17*-strep

    Plasmid

    #120167

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF17-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 17 (KIF17 Human)
    

    Use

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF17: 1-509 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCh-KIF13A*-strep

    Plasmid

    #120166

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF13A-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 13A (KIF13A Human)
    

    Use

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF13A: 1-639 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mCh-KIF5A*-strep

    Plasmid

    #120163

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 5A (Kif5a Mouse)
    

    Use

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF5A: 1-559 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-PPO-mScarlet

    Plasmid

    #139503

    Purpose

    Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Insert

    parapinopsin
    

    Use

    Tags

    mScarlet following Gly-Gly-Ser linker

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBabe-TetCMV-puro-mCherry-PA-Rac1

    Plasmid

    #22035

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mCherry-PA-Rac1 (RAC1 Avena sativa (oat), Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    Tet-CMV

    Available Since

    Sept. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Tom20-mChF-AIP(TA)

    Plasmid

    #61513

    Purpose

    Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TA mutation

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    FK506 binding protein 1A (FKBP1A Human)
    

    Use

    Tags

    AIP with TA mutation (see comments), Tom20, and m…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only