We narrowed to 9,797 results for: otos

1. pSems-leader-mXFPm-IFNGR1(18-489)

   Plasmid

   #192786

   Purpose

   Expression of mXFPm-tagged IFNGR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   IFNGR1 (IFNGR1 Human)
   

   Use

   Tags

   Ig k-chain leader sequence and mXFPm

   Expression

   Mammalian

   Mutation

   mXFPm: tryptophan 66 to phenylalanine, gluatmic a…

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAVS1 CAG rtTA3 TauWT 2N4R-EGFP

   Plasmid

   #132389

   Purpose

   Exchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   human TauWT 2N4R-EGFP (MAPT Human, Synthetic)
   

   Use

   CRISPR and Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   TREtight

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

   Plasmid

   #132393

   Purpose

   Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   human TauP301L 2N4R-EGFP (MAPT Human, Synthetic)
   

   Use

   CRISPR and Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Proline 301 to Leucine (P301L)

   Promoter

   TREtight

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSems-leader-mXFPe-IFNGR2(30-337)

   Plasmid

   #192787

   Purpose

   Expression of mXFPe-tagged IFNGR2 at the plasma membrane, e.g. for single molecule fluorescence microscopy

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   IFNGR2 (IFNGR2 Human)
   

   Use

   Tags

   Ig k-chain leader sequence and mXFPe

   Expression

   Mammalian

   Mutation

   mXFPe: tyrosine 66 to phenylalanine, asparagine 1…

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-CAG-FLEx-iSeroSnFR-EnhancedExport

   Plasmid

   #129180

   Purpose

   Fluorescent reporter for serotonin imaging (conditional + membrane localization tag with enhanced export sequence)

   Depositor

   Lin Tian

   Article

   Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

   Insert

   iSeroSnFR
   

   Use

   AAV

   Tags

   Ig-kappa leader, Myc, and PDGFR + enhanced ER exp…

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557)

   Plasmid

   #187844

   Purpose

   Expression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   IFNAR1 (IFNAR1 Human)
   

   Use

   Tags

   Ig k-chain leader sequence, SNAPf-tag, and meGFPe…

   Expression

   Mammalian

   Mutation

   meGFPe: asparagine 198 to aspartic acid and tyros…

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSems-leader-SNAPf-mXFPe-IFNAR1(28-557)

   Plasmid

   #192785

   Purpose

   Expression of SNAPf and mXFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   IFNAR1 (IFNAR1 Human)
   

   Use

   Tags

   Ig k-chain leader sequence, SNAPf-tag, and mXFPe

   Expression

   Mammalian

   Mutation

   mXFPe: tyrosine 66 to phenylalanine, asparagine 1…

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-CMV-LNI-bGH

   Plasmid

   #233203

   Purpose

   AAV expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogramming

   Depositor

   Kate Galloway

   Article

   Wang et al Cell Syst. 2025 Mar 10, Volume 16, Issue

   Insert

   mLhx3-P2A-mNgn2x3HA-T2A-Isl1 (Neurog2, Lhx3, Isl1 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMXs-NIL (with mNgn2x3HA)

   Plasmid

   #233192

   Purpose

   Retroviral expression of Ngn2x3HA, Isl1, and Lhx3 for motor neuron reprogramming

   Depositor

   Kate Galloway

   Article

   Wang et al Cell Syst. 2025 Mar 10, Volume 16, Issue

   Insert

   mNgn2x3HA-P2A-Isl1-T2A-mLhx3 (Neurog2, Isl1, Lhx3 Mouse)
   

   Use

   Retroviral

   Tags

   x3HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXs-NLI (with mNgn2x3HA)

    Plasmid

    #233193

    Purpose

    Retroviral expression of Ngn2x3HA, Lhx3, and Isl1 for motor neuron reprogramming

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Mar 10, Volume 16, Issue

    Insert

    mNgn2x3HA-P2A-mLhx3-T2A-Isl1 (Neurog2, Isl1, Lhx3 Mouse)
    

    Use

    Retroviral

    Tags

    x3HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMXs-LIN (with mNgn2x3HA)

    Plasmid

    #233194

    Purpose

    Retroviral expression of Lhx3, Isl1, and Ngn2x3HA for motor neuron reprogramming

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Mar 10, Volume 16, Issue

    Insert

    mLhx3-P2A-Isl1-T2A-mNgn2x3HA (Neurog2, Isl1, Lhx3 Mouse)
    

    Use

    Retroviral

    Tags

    x3HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMXs-ILN (with mNgn2x3HA)

    Plasmid

    #233196

    Purpose

    Retroviral expression of Isl1, Lhx3, and Ngn2x3HA for motor neuron reprogramming

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Mar 10, Volume 16, Issue

    Insert

    Isl1-P2A-mLhx3-T2A-mNgn2x3HA (Neurog2, Isl1, Lhx3 Mouse)
    

    Use

    Retroviral

    Tags

    x3HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMXs-INL (with mNgn2x3HA)

    Plasmid

    #233197

    Purpose

    Retroviral expression of Isl1, Ngn2x3HA, and Lhx3 for motor neuron reprogramming

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Mar 10, Volume 16, Issue

    Insert

    Isl1-P2A-mNgn2x3HA-T2A-mLhx3 (Neurog2, Isl1, Lhx3 Mouse)
    

    Use

    Retroviral

    Tags

    x3HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557)

    Plasmid

    #192704

    Purpose

    Expression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    IFNAR1 (IFNAR1 Human)
    

    Use

    Tags

    Ig k-chain leader sequence, SNAPf-tag, and meGFPm…

    Expression

    Mammalian

    Mutation

    meGFPm: gluatmic acid 142 to asparagine and histi…

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)

    Plasmid

    #192784

    Purpose

    Expression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    IFNAR1 (IFNAR1 Human)
    

    Use

    Tags

    Ig k-chain leader sequence, SNAPf-tag, and mXFPm

    Expression

    Mammalian

    Mutation

    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCSC-NGN2-IRES-ISL1-T2A-LHX3

    Plasmid

    #221814

    Purpose

    Lentiviral vector expressing human NEUROG2, ISL1 and LHX3 for inducing pluripotent stem cells into cholinergic motor neurons.

    Depositor

    Baojin Ding

    Article

    Sepehrimanesh et al Am J Physiol Cell Physiol. 2020 Oct 1;31

    Inserts

    NEUROG2 (NEUROG2 Human)
    ISL1 (ISL1 Human)
    LHX3 (LHX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUCM-CLYBL-hNIL

    Plasmid

    #105841

    Purpose

    Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron

    Depositor

    Michael Ward

    Article

    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    mCherry
    rtTA3G
    

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAG, EF-1alpha, and TRE3G

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PB-EEA-5g-OSK2M2L1-PGK-Puro

    Plasmid

    #102909

    Purpose

    PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB-GG-OMKSL-PGK-Puro

    Plasmid

    #102894

    Purpose

    PiggyBac transposon system construct with 10 concatenated U6 promoter driven transcriptional cassettes targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Contains PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    OMKSL-gRNAs-PGK-Puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GG-EBNA-OMKSL-PP

    Plasmid

    #102899

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    OMKSL-gRNAS-PGK-puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge