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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,217 results for: sgRNA

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Showing: 2041 - 2060 of 9217 results

  2. Tol2-U6.3-sgRNA-non-targeting-control -GFP

    Plasmid
    #221844
    Purpose
    Tol2 transposon expresses control non-targeting sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter
    Depositor
    Stephen Terry
    Publication
    O'Sullivan et al Development. 2024 Aug 1;151(15):dev20284
    Inserts
    EGFP
    non-targeting control sgRNA- GCACTGCTACGATCTACACC
    Use
    CRISPR; Tol2 transposon optimised for chick expre…
    Expression
    Mammalian
    Promoter
    GACG and U6.3 chick
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    221844-plasmid-map-sequence-id-437475

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  6. pU6_(GLuc)_TOP1

    Plasmid
    #68423
    Purpose
    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoter
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TOP1 construct
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68423-plasmid-map-sequence-id-125554

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  12. pBM019

    Plasmid
    #128179
    Purpose
    Construct for generating stable Cas9-expressing Toxoplasma gondii
    Depositor
    Sebastian Lourido
    Publication
    Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Promoter
    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…
    Available since
    July 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    128179-plasmid-map-sequence-id-249095

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  14. px330-CD4

    Plasmid
    #136938
    Purpose
    NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDH
    Depositor
    Jeremy Stark
    Publication
    Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting CD4 (CD4 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    136938-plasmid-map-sequence-id-272090

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  17. pX330-PITCh-LMNB1

    Plasmid
    #207770
    Purpose
    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    207770-plasmid-map-sequence-id-416199

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Showing: 2041 - 2060 of 9217 results