We narrowed to 8,727 results for: sgRNA

1. pX459_gRNA-ROSA26_hspCas9

   Plasmid

   #193310

   Purpose

   Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   not applicable

   Promoter

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR-U6-gRNA-miniCMV-TdTomato

   Plasmid

   #192656

   Purpose

   A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   TdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, miniCMV

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pV1200

   Plasmid

   #111429

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al Sci Adv. 2015;1(3):e1500248.

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBS-GMR-eya(shRNA)

   Plasmid

   #157991

   Purpose

   pBSII-KS(-)-based vector backbone containing a dominant eye marker for constructing Drosophila CRISPR HDR templates. Marker allows counter selection against vector integration events.

   Depositor

   Greg Beitel

   Article

   Nyberg et al Fly (Austin). 2020 Oct 5. doi: 10.1080/1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459-sgAAVS1

   Plasmid

   #184403

   Purpose

   pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   AAVS1-specific sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKHH030

   Plasmid

   #89358

   Purpose

   hU6 sgRNA cloning vector for CDKO system

   Depositor

   Michael Bassik

   Article

   Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

   Insert

   GFP, Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHAGE-EFS-N22p-3XRFPnls

   Plasmid

   #75387

   Purpose

   N22p-3XRFPnls

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   N22p
   

   Use

   Lentiviral

   Tags

   3XmCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-EFS-PCP-3XNeon-nls

   Plasmid

   #75386

   Purpose

   PCP-3XNeon-nls

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   PCP
   

   Use

   Lentiviral

   Tags

   3XmNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIKU801-CrPEPC1

   Plasmid

   #92342

   Purpose

   Expresses sgRNA for targeting CrPEPC1 gene, paromomycin resistance

   Depositor

   I-Son Ng

   Article

   Kao et al Bioresour Technol. 2017 May 4. pii: S096

   Insert

   sgRNA-CrPEPC1
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRGE31

    Plasmid

    #50929

    Purpose

    Expressing sgRNA and Cas9 in plants. The sgRNA was controlled by rice snoRNA U3 promoter

    Depositor

    Yinong Yang

    Article

    Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Rice snoRNA U3 and dual 35S promoter

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTarget12f1

    Plasmid

    #171183

    Purpose

    Constitutive expression of sgRNA without donor DNA

    Depositor

    Kohsuke Honda

    Article

    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

    Insert

    sgRNA scaffold in the CRISPR/Cas12f1 system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119(SpeI) promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-TO-MCP-3XBFPnls

    Plasmid

    #75383

    Purpose

    MCP-3XBFPnls

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    MCP
    

    Use

    Lentiviral

    Tags

    3XBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459_gRNA-AAVS1_hspCas9

    Plasmid

    #193309

    Purpose

    Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    not applicable

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCFD3-frame_selector_0

    Plasmid

    #127553

    Purpose

    Drosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-in

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    sgRNA frame 0
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6:3

    Available since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCFD3-frame_selector_1

    Plasmid

    #127554

    Purpose

    Drosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-in

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    sgRNA frame 1
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6:3

    Available since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFH102

    Plasmid

    #128207

    Purpose

    sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LBJJ382

    Plasmid

    #117518

    Purpose

    Also known as LBJJ429. PCR template for sgRNA-EF with polyT, 67bp or 192bp terminator

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:ACTA:pU6-26:sgRNA-EF:t192bp:TTAC:BpiI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAK005

    Plasmid

    #128218

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 5

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 5
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAK003

    Plasmid

    #128216

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 3

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 3
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAK004

    Plasmid

    #128217

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 4

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 4
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge