We narrowed to 6,675 results for: proc

1. pAAV.hSyanpsin.(cyto).iATPSnFR2.S29W.A95K.HaloTag

   Plasmid

   #209663

   Purpose

   Expression of iATPSnFR2, high affinity

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.S29W.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   S29W.A95K

   Promoter

   hSyn

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJAK112

   Plasmid

   #167280

   Purpose

   CodA-based mutagenesis vector for C. difficile

   Depositor

   Robert Fagan

   Article

   Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

   Type

   Empty backbone

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pWPI-IRES-eGFP-MEFV

   Plasmid

   #92103

   Purpose

   Expression in eukaryotic cells and generating stable cell lines

   Depositor

   Feng Shao

   Article

   Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

   Insert

   MEFV (MEFV Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pHAGE-UBC-TagRFP-T-ps

   Plasmid

   #178656

   Purpose

   Expression of palmitoylated TagRFP-T for live imaging of plasma membrane (protrusions, tunneing nanotubes), in mammalian cells

   Depositor

   Jeffrey Gerst

   Article

   Haimovich et al Proc Natl Acad Sci U S A. 2017 Nov 14;11

   Insert

   TagRFP-T with palmitoylation signal at N-terminus
   

   Use

   Lentiviral

   Tags

   Palmitoylation signal

   Expression

   Mutation

   Promoter

   Ubc

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV-U-TVA950

   Plasmid

   #115236

   Purpose

   Lentiviral transfer plasmid for expression of TVA950

   Depositor

   Ian Wickersham

   Article

   Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

   Insert

   TVA950
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPRIME-CMV-dsRed-FF3

   Plasmid

   #11664

   Purpose

   3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of dsRed and miR30-based shRNA targeting firefly luciferase

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Insert

   FF3
   

   Use

   Lentiviral

   Tags

   dsRed

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

7. 3492 pcDNA3 Bad

   Plasmid

   #8778

   Depositor

   Stanley Korsmeyer

   Article

   Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

   Insert

   Bad (Bad Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

8. 882 pSG5L HA PTEN G129E

   Plasmid

   #10746

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G129E

   Promoter

   Available since

   Sept. 27, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCI syn iGluSnFR

   Plasmid

   #106123

   Purpose

   Indicator for glutamate imaging (synapsin promoter)

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluSnFR
   

   Use

   Tags

   Myc and PDGFR

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBSc + MShal

    Plasmid

    #16166

    Depositor

    Larry Salkoff

    Article

    Pak et al Proc Natl Acad Sci U S A. 1991 May 15. 8

    Insert

    Mus musculus potassium voltage-gated channel (Kcnd1 Mouse)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pISH-Gap43

    Plasmid

    #74336

    Purpose

    Use to synthesize riboprobes for in situ hybridization (ISH) probe for Gap43. Not for gene expression.

    Depositor

    Peter Mombaerts

    Article

    Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

    Insert

    growth associated protein 43 (Gap43 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-CMV-NGLY1-Puro

    Plasmid

    #181916

    Purpose

    Lentiviral plasmid that directs the expression of NGLY1 in mammalian cells.

    Depositor

    Scott Dixon

    Article

    Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    N-glycanase 1 (NGLY1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEN_TTmiRc2

    Plasmid

    #25752

    Purpose

    Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJS145

    Plasmid

    #179159

    Purpose

    Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.

    Depositor

    Iva Greenwald

    Article

    Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

    Insert

    rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Flexon replacing 2nd intron of 2xNLS::GFP

    Promoter

    rps-27p

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 884 pGEX2T PTEN G129E

    Plasmid

    #10747

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G129E

    Promoter

    Available since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

16. 819 pGEX2T PTEN C124S

    Plasmid

    #10745

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C124S

    Promoter

    Available since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pHeEf1a-mEGFP-NLS

    Plasmid

    #205012

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the ef1a gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the ef1a gene from Hypsibius exemplaris
    

    Use

    Tardigrade expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRvACT-mEGFP-NLS

    Plasmid

    #205001

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Ramazzottius varieornatus
    

    Use

    Tardigrade expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCNL-N1_vinculin

    Plasmid

    #65691

    Purpose

    Expresses Vinculin-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    vinculin (Vcl Mouse)
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pAW-AlphaLobe

    Plasmid

    #113255

    Purpose

    E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

    Depositor

    Jennifer Doudna

    Article

    Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    S. pyogenes Cas9 alpha-helical lobe
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    residues 56–714; See paper for description

    Promoter

    T7

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge