We narrowed to 15,118 results for: pET

1. pET28-6His-SUMO-Nsp14

   Plasmid

   #154956

   Purpose

   Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

   Depositor

   Justin Kollman

   Insert

   6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
   

   Tags

   6His-Thrombin-SUMO

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pET-FLAG-SpCas9-HF1

   Plasmid

   #126770

   Purpose

   Expression of increased fidelity SpCas9-HF1 in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   T7

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET23-PBD(65-150)-N-Cys

   Plasmid

   #13722

   Depositor

   Klaus Hahn

   Article

   Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

   Insert

   PBD(65-150)-N-Cys (PAK1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Met-Cys-Gly-PBD(65-150)-

   Available Since

   March 28, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pET28a-SENP5 (catalytic domain)

   Plasmid

   #16358

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP5 catalytic domain (SENP5 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 567 to 755 catalytic domain of human S…

   Available Since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pET22b-mEB2-eGFP-6xHis

   Plasmid

   #130982

   Purpose

   Bacterial expression of mouse EB2-GFP-6xHis for protein purification.

   Depositor

   Anne Straube

   Article

   Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

   Insert

   EB2-GFP-6xHis (Mapre2 Mouse)
   

   Tags

   eGFP-6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28b-MBP-TEV-PWWP_DNMT3A

   Plasmid

   #186970

   Purpose

   Bacterial expression of human DNMT3A PWWP domain (residues 278–427) with His-MBP tag and TEV protease cleavage site.

   Depositor

   Brian Liau

   Article

   Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

   Insert

   DNMT3A PWWP domain (DNMT3A Synthetic, Human)
   

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   PWWP domain (residues 278–427)

   Promoter

   T7

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET151-D-TOPO-PARP14-SΔK

   Plasmid

   #216658

   Purpose

   Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.

   Depositor

   Herwig Schüler

   Article

   Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.

   Insert

   PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Synthetic, Human)
   

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Mutation

   Deletion starting from Q1390 to D1448 and substit…

   Promoter

   T7

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET51b(+)_LAMA-G97 (for GFP)

   Plasmid

   #130715

   Purpose

   Plasmid encoding the bacterial expression of LAMA-G97 for GFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   LAMA-G97
   

   Tags

   Hisx10

   Expression

   Bacterial

   Mutation

   Deleted first Met, Ala and Last Ser from the Enha…

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET22b 6His SpyCatcher-GRFT

   Plasmid

   #204502

   Purpose

   Bacterial expression of GRFT with N-terminal SpyCatcher fusion and GS linker

   Depositor

   Urartu Seker

   Article

   Ozkul et al Adv Mater Interfaces. 2022 Sep 13:220112

   Insert

   GRFT
   

   Use

   Synthetic Biology

   Tags

   6xHis and SpyCatcher

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETM10 with rat FL Annexin A11

    Plasmid

    #164496

    Purpose

    For bacterial expression of rat His-tagged FL Annexin A11

    Depositor

    Anni Vedeler

    Article

    Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

    Insert

    rat FL Annexin A11 (Anxa11 Rat)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETDuet RagA-RagC(D181N)

    Plasmid

    #99643

    Purpose

    Bacterial expression of RagA-RagC(D181N)

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D181N

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-his6-HA3-HALO

    Plasmid

    #188943

    Purpose

    T7 expression vector for making his6- and HA3-tagged HALO protein

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    his6-HA3-HALO
    

    Expression

    Bacterial

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a_DmKHC[1-421]-SNAP-6xHis

    Plasmid

    #196975

    Purpose

    Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tag

    Depositor

    Lukas Kapitein

    Article

    Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Tags

    6xHis and SNAP

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-StrepII-TagRFP-CohI

    Plasmid

    #58710

    Purpose

    Detection construct to label dockerin type I containing fusion proteins

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    TagRFP-CohI
    

    Tags

    StrepII

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET30a-His6-ABP-LOXCATmut

    Plasmid

    #139972

    Purpose

    A control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Mutation in H265A and R268A in LOX (used original…

    Promoter

    T7

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETconNK-TEM1(S70A,D179G)

    Plasmid

    #81166

    Purpose

    pETconNK with TEM1(S70A,D179G) beta-lactamase

    Depositor

    Timothy Whitehead

    Article

    Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

    Insert

    TEM-1 beta-lactamase
    

    Tags

    c-myc

    Expression

    Bacterial and Yeast

    Mutation

    Deleted the sec transport tag. Plasmid encodes H2…

    Promoter

    GAL1 and gal1

    Available Since

    Feb. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Pet15b-Endosomal Escape Control

    Plasmid

    #186552

    Purpose

    Negative Control confirming the need for a CPP peptide in mediating internalization

    Depositor

    Jean Gariepy

    Article

    Mohammed et al J Control Release. 2012 Nov 28;164(1):58

    Insert

    6H-ETA-GFP
    

    Tags

    6xHis and EGFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Pet15b-Internalization and Endosomal Escape

    Plasmid

    #186551

    Purpose

    Internalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cells

    Depositor

    Jean Gariepy

    Article

    Mohammed et al J Control Release. 2012 Nov 28;164(1):58

    Insert

    10R-8His-ETA-EGFP
    

    Tags

    8xHis and EGFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET22-His-TEV-DOT1L-MM

    Plasmid

    #89611

    Purpose

    Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416

    Depositor

    Leemor Joshua-Tor

    Article

    Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

    Insert

    Dot1L VVEL293MM Mutant (DOT1L Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET30a-SARS-CoV2-NP-Nt(47-173)

    Plasmid

    #165091

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pET22b-mEB3-mCherry-6xHis

    Plasmid

    #130985

    Purpose

    Bacterial expression of mouse EB3-mCherry-6xHis for protein purification.

    Depositor

    Anne Straube

    Article

    Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

    Insert

    EB3-mCherry-6xHis (Mapre3 Mouse)
    

    Tags

    mCherry-6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET21a(+)-Histag-Cerulean (526)

    Plasmid

    #70716

    Purpose

    Expression vector for Cerulean purification

    Depositor

    Richard Murray

    Article

    Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

    Insert

    Cerulean
    

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET-tTALE-mStrawberry-15b

    Plasmid

    #206868

    Purpose

    Express tTALE-mStrawberry

    Depositor

    Kyubong Jo

    Article

    Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

    Insert

    7-mer tTALE-mStrawberry
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET28b-LanCL2 (6xHis-LanCL2)

    Plasmid

    #73407

    Purpose

    Expresses His tagged LanCL2

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    LanCL2 (LANCL2 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pET28a(+) 6xHis-TEV-IFIT3

    Plasmid

    #53559

    Purpose

    bacterial expression of IFIT3

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT3 (IFIT3 Human)
    

    Tags

    6xHIS and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pcDNA3-AMPKsub-YPet-NES

    Plasmid

    #84635

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub-YPet-NES
    

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pET51b-His-TEV-HaloTag7_dead_mt

    Plasmid

    #167267

    Purpose

    Overexpression of the dead variant (D106A) of HaloTag7 in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    HaloTag7
    

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    D106A

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pTS2396-Tier1-PhCMV-4xCOX8_Ypet

    Plasmid

    #169534

    Purpose

    Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven miochondria-localized yellow fluorescent protein Ypet
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET28a-FKBP-STING(pLxIS)

    Plasmid

    #221333

    Purpose

    Overexpression of STING CTT in bacteria

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    STING CTT (Sting1 Mouse)
    

    Tags

    6xHis-4xFKBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET21a-Med25-VBD-6His

    Plasmid

    #64773

    Purpose

    Bacterial expression of His-tagged Med25-VBD

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    Med25-VBD (MED25 Human)
    

    Expression

    Bacterial

    Mutation

    insert is 394-543 only

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only