We narrowed to 17,355 results for: She

1. pcDNA3.3_SARS2_BA.2.87.1

   Plasmid

   #218679

   Purpose

   expressing SARS-CoV-2 BA.2.87.1 spike protein for pseudovirus production

   Depositor

   David Nemazee

   Insert

   Spike (BA.2.87.1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-ROSAr

   Plasmid

   #54324

   Purpose

   TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK mCherryROSAbsr plasmid

   Depositor

   Silvestro Conticello

   Insert

   TALEN for the murine ROSA26 locus
   

   Use

   Mouse Targeting and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGG

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pY128 (pcDNA3.1-U6-huMb2Cpf1)

   Plasmid

   #92304

   Purpose

   Expression of humanized Mb2Cpf1 and crRNA

   Depositor

   Feng Zhang

   Insert

   huMb2Cpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. mPA-GFP-Lifeact-7

   Plasmid

   #54671

   Purpose

   Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   mPA-GFP-Lifeact

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pLenti_P2A_Blast

   Plasmid

   #211365

   Purpose

   Lentiviral expression vector for an insert of interest linked to a P2A-T2A blasticidin sequence. Produces virus very efficiently. Can also be used for regular transfections

   Depositor

   Jacques Neefjes

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSFDuet-galK

   Plasmid

   #193727

   Purpose

   Contains expression cassette for E.coli galK for use as a counterselection marker in strains lacking galK

   Depositor

   Martin Buck

   Insert

   galK (galK E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23113

   Available since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLIX403-ccdB-NatMx

   Plasmid

   #158563

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-His6-Sumo-MBP-CfaN

   Plasmid

   #126016

   Purpose

   Bacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)

   Depositor

   Thomas Muir

   Insert

   His5-Sumo-MBP-CfaN
   

   Use

   Tags

   5xHis-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-m5HT6-EGFP

   Plasmid

   #200443

   Purpose

   Lentiviral expression of mouse m5HT6-EGFP

   Depositor

   Ken-Ichi Takemaru

   Insert

   5HT6-EGFP
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   EF1α

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pY129 (pcDNA3.1-U6-huMb3Cpf1)

    Plasmid

    #92305

    Purpose

    Expression of humanized Mb3Cpf1 and crRNA

    Depositor

    Feng Zhang

    Insert

    huMb3Cpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBAD-cyScarlet

    Plasmid

    #191260

    Purpose

    Bacterial expression plasmids for Long Stokes shift red fluorescent protein cyScarlet

    Depositor

    Robert Campbell

    Insert

    cyScarlet
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Flag-TRIM24-F979A/N980A

    Plasmid

    #28142

    Depositor

    Michelle Barton

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    Tags

    FLAGX2

    Expression

    Mammalian

    Mutation

    F979A and N980A

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pTre-tight-SV40-luciferase (496)

    Plasmid

    #65489

    Purpose

    Inducible expression of coding/noncoding genes

    Depositor

    Rory Johnson, Roderic Guigo

    Insert

    SV40-luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight Tre promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTXB3-Flag PLK1

    Plasmid

    #68271

    Purpose

    bacterial expression of Plk1

    Depositor

    Stefano Ferrari

    Insert

    PLK1 (PLK1 Human)
    

    Use

    Tags

    Flag and Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pUAST tdTomato Profilin WT

    Plasmid

    #53509

    Depositor

    Matthew Scott

    Insert

    profilin (chic Fly)
    

    Use

    Tags

    tdtomato

    Expression

    Insect

    Mutation

    Promoter

    GAL4

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pBV58

    Plasmid

    #204603

    Purpose

    ERG11-3X Minidegron-5X FLAG-HIS3MX6

    Depositor

    Scott Moye-Rowley

    Insert

    Candida glabrata ERG11 (ERG11 )
    

    Use

    Tags

    auxin-inducible degron

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB CAG-F-bpA-2xSV40pA-F-td-sfGFP

    Plasmid

    #133572

    Purpose

    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 2x SV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Tandem dimer superfolder GFP
    

    Use

    Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PB CAG-L-bpA-3xSV40pA-L-td-sfGFP

    Plasmid

    #133577

    Purpose

    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 3x SV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Tandem dimer superfolder GFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTRIPZ (M)-YFP-Eed

    Plasmid

    #82512

    Purpose

    Lentivirus vector. Expresses YFP-Eed fusion proteins in mammalian cells.

    Depositor

    Xiaojun Ren

    Insert

    embryonic ectoderm development
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    insert contains AA39-441 of EeD

    Promoter

    cmv

    Available since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUPRT::Floxed DHFR-TS*

    Plasmid

    #100606

    Purpose

    TgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.

    Depositor

    David Sibley

    Insert

    TgUPRT::Floxed TgDHFR-TS*
    

    Use

    Toxoplasma expression

    Tags

    Expression

    Mutation

    Pyrimethamine resistance

    Promoter

    pTgDHFR-TS

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. HT-ELK1

    Plasmid

    #33258

    Depositor

    Frederick Stanley

    Insert

    ELK1 (ELK1 Human)
    

    Use

    Tags

    Halo Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

22. PB CAG-F-bpA-3xSV40pA-F-td-sfGFP

    Plasmid

    #133573

    Purpose

    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 3x SV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Tandem dimer superfolder GFP
    

    Use

    Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET3a-DJ1

    Plasmid

    #51489

    Purpose

    Inducible expression of human DJ1 in E.coli

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 31, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. Clover2-C1

    Plasmid

    #54711

    Purpose

    Localization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 H231L based on Bajar et. al. Sci Rep. 2016.

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    Clover2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

25. PB CAG-L-bpA-2xSV40pA-L-td-sfGFP

    Plasmid

    #133576

    Purpose

    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 2x SV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Tandem dimer superfolder GFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSAS)

    Plasmid

    #137864

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAGSAS N-terminus)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBEST-pTar-UTR1-TetR-T500

    Plasmid

    #45780

    Depositor

    Vincent Noireaux, Richard Murray

    Insert

    Tetracycline repressor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTar

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKK1 -pFlp-18::mito4x-GCaMP6f

    Plasmid

    #194969

    Purpose

    Fluorescent reporter of mitochondrial matrix Ca2+.

    Depositor

    Frederic Hoerndli

    Insert

    4 repeats of Cox8 presequence plus GCaMP6f
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Flp-18

    Available since

    June 21, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pMZ376

    Plasmid

    #74213

    Purpose

    Combined sgRNA/Cas9 vector with KanMX marker

    Depositor

    Mikel Zaratiegui

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    adh1 and rrk1

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-(neg27)GFP-GGS9-Cre-6xHis

    Plasmid

    #89253

    Purpose

    Expresses GFP with -27 theoretical charge fused to Cre in E.Coli

    Depositor

    David Liu

    Insert

    (neg27)GFP-GGS9-Cre-6xHis
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. sCFP in pBSDONR P4r-P2

    Plasmid

    #121530

    Purpose

    For making C-terminal sCFP fusions using multisite Gateway cloning

    Depositor

    Roger Innes

    Insert

    sCFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pUltra-Smurf-luc-RTTA3

    Plasmid

    #59438

    Purpose

    silence doxycyclin inducible pUltra-dox (also expresses luciferase)

    Depositor

    Kitai Kim, Yildirim Dogan

    Inserts

    luciferase
    Reverse TET-Transactivator 3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    loxP5171

    Promoter

    Available since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHAGE2-RSV-jGCaMP7s-W

    Plasmid

    #126690

    Purpose

    Mammalian calcium sensor protein.

    Depositor

    Darrell Kotton

    Insert

    jGCaMP7s
    

    Use

    Lentiviral

    Tags

    6xHIS, T7, Xpress

    Expression

    Mutation

    A52V, A317L

    Promoter

    RSV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLV Tor1a-V5

    Plasmid

    #175135

    Purpose

    Lentiviral expression of Tor1a-Myc

    Depositor

    Larry Gerace

    Insert

    Tor1a (Tor1a Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHAGE2-RSV-jGCaMP7f-W

    Plasmid

    #126691

    Purpose

    Mammalian calcium sensor protein.

    Depositor

    Darrell Kotton

    Insert

    jGCaMP7f
    

    Use

    Lentiviral

    Tags

    6xHIS, T7, Xpress

    Expression

    Mutation

    H78K, A317L

    Promoter

    RSV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBluescript mouse ptc

    Plasmid

    #58699

    Purpose

    full length mouse ptc cDNA

    Depositor

    Matthew Scott

    Insert

    ptc (Ptch1 Mouse)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pMC6-TEFkFLP(LYS2)

    Plasmid

    #49458

    Purpose

    overespression of Flp step-arrest mutant in yeast, LYS2 selection

    Depositor

    Marc Gartenberg

    Insert

    Flp(H305L) (flp Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H305L, ‘step-arrest’ mutant,

    Promoter

    TEF1

    Available since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJK266

    Plasmid

    #73326

    Purpose

    Produces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    violacein biosynthesis protein VioD
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pIC81

    Plasmid

    #63065

    Purpose

    Mammalian expression of EGFP-TEV-S-tag PMFI for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    PMFI, IMAGE clone 6148250 (PMF1 Human)
    

    Use

    Tags

    EYFP and TEV-S

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PB CAG-L-bpA-SV40pA-L-td-sfGFP

    Plasmid

    #133575

    Purpose

    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 1x SV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Tandem dimer superfolder GFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge