We narrowed to 8,855 results for: plasmid dna
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Plasmid#225588PurposeMammalian expression vector for TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertT21R-F8.2(S54L+T127A)-T2A-mCherry
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO T21R-F8.2 (S54L+T127A)-T2A-mCherry
Plasmid#225584PurposeMammalian expression vector for Doxycycline inducible expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertT21R-F8.2 (S54L+T127A)-T2A-mCherry
UseTagsmCherryExpressionMammalianMutationT21R-F8.2 (S54L+T127A)PromoterCMVAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-puro-kozak-scFv-GCN4-HAtag-msfGFP(V206K)-GBI
Plasmid#205162Purposeexpress scFv-GCN4-HAtag-msfGFP(V206K)-GBIDepositorInsertscFv-GCN4-HAtag-msfGFP(V206K)-GBI
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)
Plasmid#238007Purpose300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.DepositorUseRna donor to study rt-dsbrTagsExpressionMammalianMutation3bp insertion signature (ATC) at Cas9 cut site in…PromoterCMVAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader GUG-nLuc-3XFLAG
Plasmid#127327PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader AUG-nLuc-3XFLAG
Plasmid#127326PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1755 - pcDNA3.1 CMV-IE hChR2(H134R)-V5-ER-LBD (ChRERa)
Plasmid#201819PurposeA plasmid expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CMV promoterDepositorInserthChR2(H134R)-V5-ER-LBD (ChRERa)
UseTagsExpressionMammalianMutationPromoterpOTTC1751Available sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 V5 His-TOPO-GluCl optalpha-mYFP L9'F (Opt α-GluClv2.0)
Plasmid#47387Purposeallow pharmacologically induced silencing of electrical activity in CNS neurons upon exposure to the anthelmintic drug ivermectinDepositorInsertglutamate-gated chloride Channels
UseTagsExpressionMammalianMutationLeu9 prime mutated to F (Phenylalanine in M3-M4 l…PromoterCMVAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry
Plasmid#124429PurposeExpresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cellsDepositorInsertFlipGFP(TEV cleavage seq) T2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PS11-scFvFc-CD28-gp41 (706-713)
Plasmid#60606PurposeMammalian Display vectorDepositorInsertPS11-scFvFc-CD28-gp41 (706-713) (CD28 Human)
UseTagsC9ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST
Plasmid#127332PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRESDepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Zeo(+)_mCherry_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor
Plasmid#182685PurposeEncodes mCherry and contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Can be used as a donor plasmid for the tagging of endogenous NFL via TKIT approach.DepositorInsertsUseTagsExpressionMammalianMutationPromoterCMV and NAAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)
Plasmid#47542Purposeengineered receptor improves heteromeric receptor expression and sensitivity to IVM.DepositorInsertGlutamate-gated chloride channels
UseTagsExpressionMammalianMutationretention motif in ER (starting at R340) changed …PromoterCMVAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(TAG)-3xFLAG donor
Plasmid#182686PurposeContains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linkerA6TAG-3xFLAG. Can be used as a donor for the tagging of endogenous NFL via TKIT approach and click labeling of NFL.DepositorInsertmouse Nefl intron3-exon4-linker(TAG)-3xFLAG donor sequence (Nefl Mouse)
UseTagsExpressionMammalianMutationPromoterNAAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)
Plasmid#47542Purposeengineered receptor improves heteromeric receptor expression and sensitivity to IVM.DepositorInsertGlutamate-gated chloride channels
UseTagsExpressionMammalianMutationretention motif in ER (starting at R340) changed …PromoterCMVAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
CelTag Plasmid
Plasmid#66562PurposeEncodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.DepositorInsertCelTag
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
CelTag Plasmid
Plasmid#66562PurposeEncodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.DepositorInsertCelTag
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
CJ8421_04975 Rptr2 recording plasmid
Plasmid#194211PurposeCJ8421_04975 Rptr2 targeted DNA sequenceDepositorInsertCJ8421_04975 Rptr2 DNA target
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
T7 promoter + rpsL reporter plasmid
Plasmid#156455PurposeA mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.DepositorInsertsrpsL
TcR
UseTagsExpressionBacterialMutationATG start codon mutated to ACGPromotertac and tetAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-SpRYgest_targetplasmid (KAC833)
Plasmid#181748PurposepUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.DepositorTypeEmpty backboneUseSubstrate plasmidTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only