We narrowed to 11,533 results for: plasmid dna
-
Plasmid#131255PurposeMammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
TagsMycExpressionMammalianMutationA mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 H2B-mIFP T2A Mpro (wt)
Plasmid#163079PurposeExpresses wild type Mpro (active) and a near-infrared fluorescent protein mIFP fused to H2BDepositorInsertWild type Mpro of SARS-CoV-2 and H2B-mIFP
ExpressionMammalianAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 IRF7(5’UTR)-FF
Plasmid#85490PurposeFirefly luciferase under the control of IRF7 5'UTRDepositorAvailable SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 NDUFS6(5’UTR)-FF
Plasmid#85488PurposeFirefly luciferase under the control of NDUFS6 5'UTRDepositorAvailable SinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT-HA-hM3D(Gq)
Plasmid#45547PurposeExpresses hM3D (Gq) neuronal activatorDepositorInserthM3D (Gq)
TagsHAExpressionBacterial and MammalianPromoterCMVAvailable SinceJuly 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT-HA-hM4D(Gi)
Plasmid#45548PurposeExpresses hM4D (Gi) neuronal inhibitorDepositorInserthM4D (Gi)
TagsHAExpressionBacterial and MammalianPromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-KIRIN1
Plasmid#107416PurposeMammalian cell expression plasmid for potassium ion ratiometric indicator KIRIN1 with nuclear exclusion sequenceDepositorInsertKIRIN1
TagsNuclear exclusion sequence (NES) (LALKLAGLDIGS)ExpressionMammalianAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoA-91-NLuc
Plasmid#207151PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
ExpressionMammalianMutationNLuc inserted at position L(91)GAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoB-91-NLuc
Plasmid#207152PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
ExpressionMammalianMutationNLuc inserted at position L(91)GAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SM-FLuc0-malat1
Plasmid#210580PurposeExpresses SM(FLAG)-nonoptimal Firefly - 3'UTR malat1 triple helixDepositorInsertSpaghetti monster (FLAG)-nonoptimal FLuc - 3'UTR malat1 triple helix
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianPromoterCMVAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SM-FLuc100-malat1
Plasmid#210581PurposeExpresses SM(FLAG)-optimal Firefly luciferase - 3'UTR malat1 triple helixDepositorInsertSpaghetti monster (FLAG)-optimal FLuc - 3'UTR malat1 triple helix
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianPromoterCMVAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-H2-18 Light chain
Plasmid#216308PurposeExpresses anti-HER2 H2-18 Light chain in mammalian cells. To be paired with H2-18 heavy chain to form the anti-HER2 H2-18 FabDepositorInsertH2-18 Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ShhN-Ren
Plasmid#37677DepositorInsertshh (Shh Mouse)
Tagsrenilla luciferaseExpressionMammalianMutationsoluble form (see comments)PromoterCMVAvailable SinceNov. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
DNA-CARtcr-IRESpuro
Plasmid#89346Purpose2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassetteDepositorInsertTCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
UseLentiviralTagsSNAPf tagExpressionMammalianPromoterSFFVAvailable SinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Neo-LUC
Plasmid#214368PurposeExpression of the firefly luciferase ProteinDepositorInsertLUC
ExpressionMammalianPromoterCMVAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_LynSNAP IRES EGFP
Plasmid#136625PurposePlasmid encoding outer inner membrane localization of SNAP-Tag and EGFPDepositorInsertLyn11-SNAP-Tag (IRES EGFP)
ExpressionMammalianPromoterCMVAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gα13(EE)-YFP
Plasmid#171374PurposePlasmid encoding Gα13 internally-tagged with the Citrine variant of YFP in the b/c loopDepositorInsertGa13(EE)-YFP
TagsGlu-GluExpressionMammalianPromoterCMVAvailable SinceNov. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1_ spike_del19
Plasmid#155297PurposeExpression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particlesDepositorInsertSARS-Cov2 spike_deleted (S SARS-Cov2)
UseGeneration of sars-cov-2-pseudotyped lentiviral p…MutationDeletion in C-term 19 aa of spikePromoterCMVAvailable SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-CXCR4
Plasmid#98942PurposeMammalian expression plasmid for untagged human CXCR4DepositorAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hACE2
Plasmid#145033PurposeExpress human ACE2 with C9 tag at C-terminal in mammalian cellsDepositorAvailable SinceApril 15, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits