We narrowed to 8,791 results for: Plat;

1. LC101: pMVP/PB/Neo-DEST

   Plasmid

   #121875

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ Neo selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-hSyn-Galpha-s-IRESWT-Gbetagamma ONE-GO

   Plasmid

   #204342

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   hSyn Galpha-s/Gbetagamma ONE-GO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. LLP787_SnoopCatcher-EZH2-FL-CO

   Plasmid

   #211780

   Purpose

   SnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   aGCN4-DNMT3A
   

   Use

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect DNMT3A…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. LLP780_SpyCatcher-KRAB-CO

   Plasmid

   #211773

   Purpose

   SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional repressor KRAB, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   SpyCatcher-KRAB
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect KRAB e…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-C-Solo-mScarlet-H2BC11

   Plasmid

   #207756

   Purpose

   Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pHJ4: LexA-VHH-Caffeine

   Plasmid

   #108242

   Purpose

   LexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression.

   Depositor

   Jerome Bonnet

   Article

   Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

   Insert

   LexA DNA binding domain fusing with VHH-Caffeine antibody
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   pLacO1

   Available since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. TIMP1-bio-His

   Plasmid

   #52089

   Purpose

   Expresses full-length Metalloproteinase inhibitor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   TIMP1 (TIMP1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS

   Plasmid

   #107319

   Purpose

   Expresses SpCas9 fused to dSaCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-SaCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 is fused to nuclease dead SaCas9 (D10A, N5…

   Promoter

   CMV IE94

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOP/SSAT

   Plasmid

   #140291

   Purpose

   Expresses human SSAT in bacterial cells

   Depositor

   Gaetano Marverti, Saverio Bettuzzi

   Article

   Marverti et al Carcinogenesis. 2005 Oct;26(10):1677-86.

   Insert

   SSAT1 (SAT1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. TIMP3-bio-His

    Plasmid

    #53418

    Purpose

    Expresses full-length Metalloproteinase inhibitor 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TIMP3 (TIMP3 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-hSyn-FlicR1

    Plasmid

    #74143

    Purpose

    Express FlicR1 preferentially in neurons

    Depositor

    Robert Campbell

    Article

    Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

    Insert

    FlicR1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLCHKO

    Plasmid

    #155048

    Purpose

    Lentiviral backbone for cloning and expressing U6 driven hybrid guide (hg)RNAs with BfuAI cloning sites and puromycin selection.

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. attB-mCh-Poly(A)

    Plasmid

    #65525

    Purpose

    Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attB-mCherry
    

    Use

    Mouse Targeting; Bxb1

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. MMP11-bio-His

    Plasmid

    #53408

    Purpose

    Expresses full-length Stromelysin-3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    MMP11 (MMP11 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGC(2.1)-U6.3

    Plasmid

    #170516

    Purpose

    PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Insert

    gRNAcore(2.1)-pU6.3
    

    Use

    Pcr template vector for amplifying grnacore(2.1)-…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCSD_2XNLS-SpCas9WT-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

    Plasmid

    #107324

    Purpose

    Expresses SpCas9 fused to NmCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9-NmCas9 fusion
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    SpCas9 is fused to NmCas9

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. FgH1tUTG_huBim_Ex3

    Plasmid

    #85532

    Purpose

    Inducible expression of guide RNA (huBim_Ex3) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    hu Bim
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1t

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBeast-pAhpC-RBS- LacZ

    Plasmid

    #190060

    Purpose

    LacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    lacZ
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBeast-pT7-soxA

    Plasmid

    #190057

    Purpose

    Express the bacterial monomeric sarcosine oxidase enzyme under the control of the strong constitutif phage promoter T7

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    SoxA
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MMRN1-bio-His

    Plasmid

    #53409

    Purpose

    Expresses full-length Multimerin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    MMRN1 (MMRN1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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