We narrowed to 20,614 results for: ACE
-
Plasmid#157872Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal IDRDepositorAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only
-
pCAG-AILR-LplA-ER
Plasmid#42574DepositorInsertLplA(AILR) with ER-retention sequence KDEL
TagsFLAGMutationCompared to wild-type LplA: tryptophan 37 to alan…PromoterCAGAvailable SinceApril 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMB1-B-TelR
Plasmid#204980PurposeLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialMutationAdded replications elements for Acinetobacter spe…Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVH016
Plasmid#80400PurposeNo anti-scaffold control with 3xFLAG tags for Western blots. Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG).DepositorUseSynthetic BiologyTagsC-terminal 3xFLAG tag and C-terminal LZx domainExpressionBacterialAvailable SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-006
Plasmid#78693PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyAvailable SinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHES873
Plasmid#87945PurposeExpresses estradiol-inducible synthetic transcription factor in yeast under control of GAL1 promoter. It activates transcription of promoters containing Zif268 binding sites.DepositorInsertpGAL1- Zif268 DBD - hPR LBD - MSN2 AD (PGR Budding Yeast, Human)
ExpressionYeastPromoterGAL1Available SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pGAP T-
Plasmid#161487PurposeNon targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Non targeting control NTC)
ExpressionYeastPromoterpGAP, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
RnAT1aR-mCherry
Plasmid#137782PurposeVisualization of At1aRDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-p52-HDM2_sensor
Plasmid#127184PurposeEukaryotic expression of the fusion protein used for drug diruption the p53-HDM2 interactionDepositorInsertp53-YPeT-Pro15-GGS2-Pro15-CFP-HDM2
TagsHisTag and StrepTagExpressionMammalianPromoterCMV-TetO2Available SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJin-290
Plasmid#125718PurposeABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19 with CGG RNAP-CDepositorInsertPCGG-gRNA GFP-off expression, FKBP-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-FRB
ExpressionMammalianAvailable SinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-001
Plasmid#78688PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMB1-D1-TelR
Plasmid#204983PurposeLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialMutationAdded replications elements for Acinetobacter spe…Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
P3U2C5T2-v1-2 (Pl-lacO1-tetR) (136p)
Plasmid#70212Purpose1 Part of 4-part switchDepositorInserttetR
Available SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-Bba_K822000
Plasmid#74094PurposeL-Lactate inducible promoter from E. coli MG1655DepositorInsertBba_K822000 (L-Lactate promoter - E. coli MG1655)
UseSynthetic BiologyExpressionBacterialPromoterNAAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS5
Plasmid#55750PurposeProduces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.DepositorInsertKanR
ExpressionBacterialMutationResidues 59-264 of KanRPromoterT7-lacAvailable SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pfTRAP
Plasmid#50048PurposeExpresses plasmodium falciparum TRAP adhesion domains (26-299) in mammalian cellsDepositorInsertplamodium falciparum TRAP (TRAP Synthetic)
Tags6Xhis tagExpressionMammalianMutationC55G, N132SPromoterCMVAvailable SinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pfTRAP(VWA)
Plasmid#50049PurposeExpresses plasmodium falciparum TRAP VWA domain (41-240) in mammalian cellsDepositorInsertpfTRAP(vwa) (TRAP Synthetic)
Tags6Xhis tagExpressionMammalianMutationC55G, N132SPromoterCMVAvailable SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV epArcLight
Plasmid#85803Purposegenetically-encoded fluorescent voltage indicatorDepositorInsertepArcLight
ExpressionMammalianMutationCi-VSP contains R217Q mutation; ecliptic pHluorin…PromoterCMVAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZA-mCherry-R5
Plasmid#106258PurposeExpresses mCherry fused with silaffin-R5 silica affinity peptide under the control of TetODepositorInsertmCherry
TagsAdded silaffin R5 peptide to C terminus of mCherr…ExpressionBacterialAvailable SinceOct. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Psy074
Plasmid#99504PurposeBacterial expression of LIPS M1+TEDepositorInsertLIPS M1+TE
ExpressionBacterialAvailable SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCK300
Plasmid#87766Purposeempty control plasmid, three terminators to place inserts between for insulation, ampR, pBR322 origin, lacIDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterNoneAvailable SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pGAP T5
Plasmid#161492PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting T5 in the THI11 promoter)
ExpressionYeastPromoterpGAP, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pGAP T1
Plasmid#161488PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T1_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting T1 in the THI11 promoter)
ExpressionYeastPromoterpGAP, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
attB-P7-attP-YFP
Plasmid#195571PurposeA strong P7 promoter flanked by Bxb1 recombination sites attB and attP. Downstream of attP is a strong RBS and YFP coding sequence.DepositorInsertPromoter flanked by Bxb1 recombination sites attB and attP with YFP downstream
UseSynthetic BiologyPromoterP7Available SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-3nitroY-A7-RS
Plasmid#141173PurposeExpression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acidsDepositorInsertA7 3nY tRNA synthetase
UseLentiviralExpressionMammalianMutationP186T L274R N311G C313NPromoterCMVAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR-35S
Plasmid#99861PurposeExpresses Cas9-Nlux and sgRNA. Contains CEN/ARS and 35 terminator. No selection marker.DepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsCas9-NluxPromoterRibi promoterAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD-SPW
Plasmid#190628PurposeBacterial expression of genetically encoded protease indicator SPWDepositorInsertSPW
ExpressionBacterialAvailable SinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMD19T-psba1-Ppsba2-dCas9-SpR
Plasmid#73220PurposeContains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.DepositorInsertdCas9 from S. pyogenes
Tagsc-mycExpressionBacterialMutationSilent mutation at bp 1341 A->C to remove an E…PromoterPpsbA2Available SinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-ldhA
Plasmid#102288PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting ldhA.DepositorInsertldhA gRNA
UseCRISPRExpressionBacterialPromoterpTetAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-004
Plasmid#78691PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-003
Plasmid#78690PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-013
Plasmid#78701PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-rpsL
Plasmid#89953PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting rpsL.DepositorInsertrpsL gRNA
ExpressionBacterialPromoterpTetAvailable SinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Pr-deCFP-MGapt
Plasmid#67740PurposeOR2-OR1-Pr promoter expressing deCFP-MG aptamer fusionDepositorInsertUTR1-deCFP-MGapt-T500
UseSynthetic BiologyExpressionBacterialPromoterOR2-OR1-PrAvailable SinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBP-pET-RBS
Plasmid#72981PurposePET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)DepositorInsertpET RBS derived from pET vectors
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-SCW
Plasmid#190633PurposeMammalian expression of genetically encoded calcium indicator SCWDepositorInsertSCW
ExpressionMammalianAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-SKW
Plasmid#190631PurposeBacterial expression of genetically encoded potassium indicator SKWDepositorInsertSKW
ExpressionBacterialAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-SCW
Plasmid#190626PurposeBacterial expression of genetically encoded calcium indicator SCWDepositorInsertSCW
ExpressionBacterialAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pr1-T7RNAP
Plasmid#67738PurposeOR2-OR1-Pr1 promoter expressing T7 RNAPDepositorInsertUTR1-T7RNAP-T500
UseSynthetic BiologyExpressionBacterialPromoterOR2-OR1-Pr1Available SinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
TrkA-mCherry-CRY2
Plasmid#106167Purposemammalian expression of full length TrkA fused to mCherry and CRY2PHR (aa 1-498)DepositorInsertTrkA mCherry CRY2
ExpressionMammalianPromoterCMVAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only