We narrowed to 13,843 results for: Cas9

1. pE-35S-miniSdd7-nCas9-PBE-GmPPO2

   Plasmid

   #204855

   Purpose

   For cytosine base editing using miniSdd7 at PPO2 site in soybean (Agrobacterium-mediated transformation)

   Depositor

   Caixia Gao

   Article

   Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

   Insert

   miniSdd7-nSpCas9(D10A)-UGI-mScarlet
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A in SpCas9

   Promoter

   35S, AtU6-26p

   Available since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-LTR-dCas9-VP64-BFP

   Plasmid

   #46912

   Purpose

   Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Inserts

   dCas9-VP64-BFP fusion
   Puromycin resistance
   

   Use

   CRISPR and Retroviral

   Tags

   3xNLS, BFP, and VP64 domain

   Expression

   Mammalian

   Mutation

   Promoter

   LTR and PGK

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

   Plasmid

   #180263

   Purpose

   Lentiviral expression vector for dCas9-VP64 in mammalian cells

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Nuclease dead Cas9

   Promoter

   SFFV

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-SFFV-dCas9-BFP-KRAB

   Plasmid

   #46911

   Purpose

   Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9-BFP-KRAB fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2xNLS, BFP, HA, and KRAB domain

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro

   Plasmid

   #192664

   Purpose

   3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance marker

   Depositor

   Jeffrey Miner

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, D839A, H840A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMV-FLEX-SaCas9-U6-sgTh

   Plasmid

   #159901

   Purpose

   Mutagenesis of Th

   Depositor

   Larry Zweifel

   Insert

   Th (Th Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Px461-Cas9n-Trp53-sgRNA-beta

   Plasmid

   #88847

   Purpose

   CRISPR KO of Trp53

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

8. Px461-Cas9n-Trp53-sgRNA-alpha

   Plasmid

   #88846

   Purpose

   CRISPR KO of Trp53

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pLV hUbC-dCas9-T2A-GFP

   Plasmid

   #53191

   Purpose

   Co-expresses human optimized S. pyogenes dCas9 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized dead Cas9 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid

    #106477

    Purpose

    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-MMLVgag-D3A-3L-dCas9-ZIM3

    Plasmid

    #240531

    Purpose

    Expresses MMLVgag–DNMT3A-3L-dCas9-ZIM3 for producing RENDER-DNMT3A-3L-dCas9-ZIM3

    Depositor

    James Nuñez

    Article

    Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

    Insert

    MMLVgag-D3A-3L-dCas9-ZIM3
    

    Use

    Tags

    FLAG, HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1-idCas9-KRAB-Hygro donor

    Plasmid

    #199621

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible dCas9-KRAB cassette to the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

    Insert

    AAVS1-idCas9-KRAB-Hygro
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1

    Plasmid

    #159905

    Purpose

    Mutagenesis of Slc32a1

    Depositor

    Larry Zweifel

    Insert

    Slc32a1 (Slc32a1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV1 for Split-PE, SpCas9 nickase (PKC1001)

    Plasmid

    #190111

    Purpose

    AAV1 for expression of nickase SpCas9(H840A) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    nSpCas9
    

    Use

    AAV

    Tags

    bpNLS

    Expression

    Mutation

    H840A

    Promoter

    EF1a

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti EF1a-FLAG-dCas9-VPR

    Plasmid

    #114195

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcs2+spCas9 ABE-Umax-nanos

    Plasmid

    #222142

    Purpose

    Adenine base editor with modified TadA variant for germline A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9 with nanos UTR
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    SP6

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-SasgRNA scaffold-CMV-nSaCas9

    Plasmid

    #171695

    Purpose

    Expresses nSaCas9 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

    Insert

    sgRNA scaffold-nSaCas9-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    U6 and CMV

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT3TS-spCas9-ABE-Umax-rest1

    Plasmid

    #222144

    Purpose

    Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-EF1alpha-VP64-dCas9-p300_Blast

    Plasmid

    #192654

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    VP64-dCas9-p300
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A; H840A in Cas9

    Promoter

    EF1a

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Fuw-dCas9-Tet1CD-P2A-BFP

    Plasmid

    #108245

    Purpose

    Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

    Insert

    dCas9-Tet1CD-HA-P2A-BFP
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    dCas9-D10A, dCas9-H840A

    Promoter

    Ubc

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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