We narrowed to 14,221 results for: sgrna

1. lentiCRISPR - Amplicon, BCR/ABL sgRNA 2

   Plasmid

   #70658

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

   Plasmid

   #71829

   Purpose

   Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   BACH2-sgRNA8 (BACH2 Synthetic, S. pyogenes, Human)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; E756A inactiva…

   Promoter

   U6

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. peSpCas9(1.1)-2×sgRNA (IFT88, donor)

   Plasmid

   #80769

   Purpose

   Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

   Insert

   IFT88 gRNA#1 (IFT88 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLG1-puro Non-targeting sgRNA 3

   Plasmid

   #109003

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   Non-targeting sgRNA #3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL1 sgRNA(MS2)-MS2-AIDmono

   Plasmid

   #112127

   Purpose

   empty sgRNA cloning vector with MS2-AIDmono

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   AID mono mutant (AICDA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   N- mutated and C- terminal truncated, H130A/R131E…

   Promoter

   hU6,CBh

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLH-sgRNA1-boxB-MS2-PP7

   Plasmid

   #75397

   Purpose

   sgRNA1-boxB-MS2-PP7

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   sgRNA1-boxB-MS2-PP7
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEJS468-pLK.O1-NmeSgRNA/DTS13-Telomere

   Plasmid

   #85714

   Purpose

   Telomere-targeting Nme sgRNA under U6 promoter

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Insert

   telomere sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-sgRNA(MS2)-puro-barcode

   Plasmid

   #89493

   Purpose

   Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

   Depositor

   Gary Hon

   Article

   Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

   Insert

   sgRNA (with MS2 loop)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSaCas9-sgRNA-Tetra-com vector

   Plasmid

   #131227

   Purpose

   For expressing gRNA, whose Tetraloop is replaced with a com aptamer

   Depositor

   Baisong Lu

   Article

   Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

   Insert

   vector for com modified sgRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA

    Plasmid

    #133339

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_ctrA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    constitutive

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLL4 sgRNA(MS2)-MS2-hA3a

    Plasmid

    #112130

    Purpose

    empty sgRNA cloning vector with MS2-humanA3a

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    human Apobec3a (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6,CBh

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLL3 sgRNA(MS2)-MS2-AID3f

    Plasmid

    #112129

    Purpose

    empty sgRNA cloning vector with MS2-AID3f

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    AIDmono 3f mutant (AICDA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    N- mutated and C- terminal truncated, F115Y/C116F…

    Promoter

    hU6,CBh

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLL2 sgRNA(MS2)-MS2-AID3c

    Plasmid

    #112128

    Purpose

    empty sgRNA cloning vector with MS2-AID3c

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    AID mono 3c mutant (AICDA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    N- mutated and C- terminal truncated, F115Y/C116F…

    Promoter

    hU6,CBh

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1-puro U6 sgRNA SOX17 -177

    Plasmid

    #50925

    Purpose

    U6 driven sgRNA targeting Sox17 -177 bp from TSS

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    Sox17 -177 sgRNA (SOX17 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9

    Plasmid

    #107857

    Purpose

    To knock-out TDP43

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    gRNA against TARDBP (Exon 1) (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLG1-puro Non-targeting sgRNA 1

    Plasmid

    #109002

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting sgRNA #1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid

    #124542

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41 C55G

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124543

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX601-miniCMV-SaCas9-U6-YFPvsSaCas9 sgRNA

    Plasmid

    #107050

    Purpose

    All-in-one vectors expressing both SaCas9 and YFP sgRNA

    Depositor

    Alex Hewitt

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1-puro U6 sgRNA Oct4A -158

    Plasmid

    #50922

    Purpose

    U6 driven sgRNA targeting OCT4 isoform A -158 bp from TSS

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    Oct4A -158 sgRNA (POU5F1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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