We narrowed to 4,524 results for: GCA

1. pAAV-Ef1a-Flex-Axon-GCaMP7b

   Plasmid

   #135419

   Purpose

   Can be used to drive axon-enriched expression of GCaMP7b in the presence of Cre recombinase.

   Depositor

   Rylan Larsen

   Insert

   axon-jGCaMP7b
   

   Use

   AAV

   Tags

   6xHis and GAP43 palmitoylation domain

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-Ef1a-Coff/Fon-GCaMP6M

   Plasmid

   #137121

   Purpose

   Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-GCaMP6M
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   Removed RSET tag

   Promoter

   EF1a

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AAV-GFAP(long)-FLEX-GCaMP7f

   Plasmid

   #189612

   Purpose

   Cre-dependent GCaMP7f under the GFAP promoter

   Depositor

   Martin Riccomagno

   Article

   Agnew-Svoboda et al Cell Rep Methods. 2022 Aug 22;2(8):10027

   Insert

   GCaMP7f
   

   Use

   AAV and Cre/Lox

   Tags

   GCaMP7f

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3

   Plasmid

   #112007

   Purpose

   For co-expression of GCaMP6s with mRuby3

   Depositor

   Lin Tian

   Article

   Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

   Has Service

   AAV9

   Insert

   GCaMP6s-P2A-mRuby3
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-Ef1a-Coff/Fon-GCaMP6F

   Plasmid

   #137124

   Purpose

   Intersectional viral expression of GCaMP6F in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-GCaMP6F
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   Removed RSET tag

   Promoter

   EF1a

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV-Syn-H2B-jGCaMP8m-WPRE

   Plasmid

   #176748

   Purpose

   AAV-mediated expression of histone fused GCaMP8m under the synapsin promoter

   Depositor

   Loren Looger

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   H2B-jGCaMP8m
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

   Plasmid

   #105715

   Purpose

   In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.

   Depositor

   Rylan Larsen

   Has Service

   AAV5

   Insert

   synaptophysin-GCaMP6s
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a, Human elongation factor-1 alpha

   Available since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAC1812_pCR8-gCasRx-CUG (control gRNA)

   Plasmid

   #118645

   Purpose

   Transient transfection; Expressed Control (CUG repeat) CasRx gRNA; Gateway Donor

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   gCasRx-CUG
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-syn-axon-jGCaMP8s-WPRE

   Plasmid

   #186043

   Purpose

   jGCaMP8s expression in axons under the control of the synapsin promoter

   Depositor

   Loren Looger

   Insert

   axon-jGCaMP8s
   

   Use

   AAV

   Tags

   GAP43-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-syn-axon-jGCaMP8m-WPRE

    Plasmid

    #186042

    Purpose

    jGCaMP8m expression in axons under the control of the synapsin promoter

    Depositor

    Loren Looger

    Insert

    axon-jGCaMP8m
    

    Use

    AAV

    Tags

    GAP43-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. RabV CVS-N2c(deltaG)-GCaMP6f

    Plasmid

    #73466

    Purpose

    Expresses GCaMP6f for calcium imaging

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    GCaMP6f
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV-syn-LifeAct-jGCaMP8m-WPRE

    Plasmid

    #186039

    Purpose

    AAV-mediated expression of LifeAct-jGCaMP8m under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    LifeAct-jGCaMP8m
    

    Use

    AAV

    Tags

    LifeAct-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV-syn-axon-jGCaMP8f-WPRE

    Plasmid

    #186041

    Purpose

    jGCaMP8f expression in axons under the control of the synapsin promoter

    Depositor

    Loren Looger

    Insert

    axon-jGCaMP8f
    

    Use

    AAV

    Tags

    GAP43-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Ai95(RCL-GCaMP6f) targeting vector

    Plasmid

    #61579

    Purpose

    Target a Cre-dependent GCaMP6-fast expression cassette to the mouse Rosa26 locus

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    CAG-LSL-GCaMP6-fast
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-syn-NES-jGCaMP8m-WPRE

    Plasmid

    #186036

    Purpose

    AAV-mediated cytosolic expression of jGCaMP8m under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    NES-jGCaMP8m
    

    Use

    AAV

    Tags

    NES-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-syn-NES-jGCaMP8s-WPRE

    Plasmid

    #186037

    Purpose

    AAV-mediated cytosolic expression of jGCaMP8f under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    NES-jGCaMP8s
    

    Use

    AAV

    Tags

    NES-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CAG-GCaMP6f-3x-miR204-5p-3x-miR122-TS

    Plasmid

    #117384

    Purpose

    An AAV genome containing miRNA target sequences (TS) 204-5p and 122 to reduce expression of GCaMP6f in astrocytes and hepatocytes, respectively

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    GCaMP6f + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA ; 3 copies of miR122 : CAAACACCATTGTCACACTCCA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. VV246: CoChR(C108S)-GCaMP6f-ER in fck

    Plasmid

    #58521

    Purpose

    Expresses CoChR(C108S) fused to GCaMP6f in mammalian cells

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    CoChR(C108S)-GCaMP6f-ER
    

    Use

    Lentiviral

    Tags

    GCaMP6f

    Expression

    Mammalian

    Mutation

    changed Cysteine 108 to Serine

    Promoter

    a-CamKII

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-AP.Adamts2.1-DIO-GCaMP8s-WPRE

    Plasmid

    #211491

    Purpose

    conditional expression of GCaMP8s under artificial promoter Adamts2

    Depositor

    Georg Keller

    Article

    O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

    Insert

    DIO-GCaMP8s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-AP.Baz1a.1-DIO-GCaMP8s-WPRE

    Plasmid

    #211493

    Purpose

    conditional expression of GCaMP8s under artificial promoter Baz1a

    Depositor

    Georg Keller

    Article

    O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

    Insert

    DIO-GCaMP8s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAV-Syn-H2B-jGCaMP8s-WPRE

    Plasmid

    #176749

    Purpose

    AAV-mediated expression of histone fused GCaMP8m under the synapsin promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    H2B-jGCaMP8s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAV-syn-NES-jGCaMP8f-WPRE

    Plasmid

    #186035

    Purpose

    AAV-mediated cytosolic expression of jGCaMP8f under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    NES-jGCaMP8f
    

    Use

    AAV

    Tags

    NES-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-Syn-nullCoChR-GCaMP6f-Kv2.1

    Plasmid

    #158758

    Purpose

    Cell body-targeted GCaMP6f, under synapsin promoter: nullCoChR (the optogenetic protein CoChR mutated to have 0 current), followed by GCaMP6f and the KV2.1 motif (nullCoChR-GCaMP6f-Kv2.1).

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    nullCoChR-GCaMP6f-Kv2.1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pENTR221-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9

    Plasmid

    #87917

    Purpose

    Gateway cloning

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    sgHTT1, sgGFP2, shCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1, U6, 7SK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-GFAP-jGCaMP7c variant 1513

    Plasmid

    #173159

    Purpose

    Expresses jGCaMP7c variant 1513 in astrocytes

    Depositor

    Hervé Chneiweiss, Vincent Vialou

    Insert

    jGCaMP7c variant 1513-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    GFAP

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV1-EF1α-F-Flex-jGCaMP7b-WPR

    Plasmid

    #171689

    Purpose

    Flp-dependent expression of jGCaMP7b

    Depositor

    Tobias Rose, Mark Huebener, Tobias Bonhoeffer

    Article

    Bauer et al Neuron. 2021 Aug 4;109(15):2457-2468.e12

    Insert

    GCaMP7b
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. VV247: sdChR(C138S)-TS-GCaMP6f-ER in fck

    Plasmid

    #58522

    Purpose

    Expresses sdChR(C138S) fused to GCaMP6f in mammalian cells

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    sdChR(C138S)-TS-GCaMP6f-ER
    

    Use

    Lentiviral

    Tags

    GCaMP6f

    Expression

    Mammalian

    Mutation

    changed Cysteine 138 to Serine

    Promoter

    a-CamKII

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pX552-hSyn-DIO-GCaMP8f_Grin1 gRNA

    Plasmid

    #215281

    Purpose

    Expresses Grin 1 gRNA in a Cre dependent GCaMP8f vector

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    Grin1 gRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    U6

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pX552-hSyn-DIO-GCaMP8f_Grin1TTT gRNA

    Plasmid

    #215282

    Purpose

    Expresses Grin1 Control gRNA in a Cre dependent GCaMP8f vector

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    Grin1 control gRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-hSyn-DIO-GCaMP6f-WPRE

    Plasmid

    #216275

    Purpose

    AAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of green fluorescent calcium indicator GCaMP6f

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    GCaMP6f
    

    Use

    AAV and Cre/Lox

    Tags

    6XHIS and Xpress

    Expression

    Mutation

    Promoter

    human Synapsin 1 (hSyn)

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-Ef1a-FAS-GCaMP6f-WPRE

    Plasmid

    #141237

    Purpose

    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    GCaMP6f
    

    Use

    AAV and Cre/Lox

    Tags

    6XHIS and Xpress

    Expression

    Mutation

    Promoter

    Human elongation factor-1 alpha (EF-1 alpha)

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-ef1a-DIO-eGCaMP(plus)

    Plasmid

    #201149

    Purpose

    AAV virus production for Cre recombinase dependent expression of eGCaMP(plus)

    Depositor

    Andre Berndt

    Article

    Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

    Insert

    eGCaMP+
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFR-mScarletI-antiFRET-P2A-GCaMP3

    Plasmid

    #191460

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) GCaMP3 to mScarlet-I fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    GCaMP3
    

    Use

    Tags

    His-mScarletI-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFR-mScarletI-antiFRET-P2A-jGCaMP7c

    Plasmid

    #191461

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) jGCaMP7c to mScarlet-I fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    jGCaMP7c
    

    Use

    Tags

    His-mScarletI-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AAV-syn-LifeAct-jGCaMP8f-WPRE

    Plasmid

    #186038

    Purpose

    AAV-mediated expression of LifeAct-jGCaMP8f under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    LifeAct-jGCaMP8f
    

    Use

    AAV

    Tags

    LifeAct-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AAV-syn-LifeAct-jGCaMP8s-WPRE

    Plasmid

    #186040

    Purpose

    AAV-mediated expression of LifeAct-jGCaMP8s under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    LifeAct-jGCaMP8s
    

    Use

    AAV

    Tags

    LifeAct-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-CaMKII-GCaMP6f-4x128-mAGNET

    Plasmid

    #166023

    Purpose

    Expresses GCaMP6f in CaMKII+ cells with low miRNA-128 expression

    Depositor

    Xue Han

    Article

    Keaveney et al J Neurosci. 2020 Dec 9;40(50):9576-9588.

    Insert

    GCaMP6f with 4 miR-128 binding sites
    

    Use

    AAV and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGP-AAV-syn-FLEX-jGCaMP8s-WPRE

    Plasmid

    #162377

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE

    Plasmid

    #162379

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMOS003: GCaMPer calcium sensor (endoplasmic reticulum)

    Plasmid

    #163047

    Purpose

    GCaMPer calcium sensor (endoplasmic reticulum)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    GCaMPer
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge