We narrowed to 626 results for: SPEN
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Plasmid#140741PurposeLentiviral vector to overexpress the autism-associated PTEN mutation D252G and GFP as two separate proteinsDepositorInsertGFP-T2A-PTEND252G (PTEN Human)
UseLentiviralTagsnoneExpressionMammalianMutationD252GPromoterhUbiCAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-PDi-CRISPRn
Plasmid#73500PurposeDox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locusDepositorInsertsCas9
rtTA
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCAG and TREAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-CKB
Plasmid#73501PurposegRNA expression vector (with mKate2)DepositorInsertmKate2-T2A-Bsd
UseCRISPR and LentiviralTagsNLSExpressionMammalianMutationPromoterCAGAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NC-CRISPRi (Gen3)
Plasmid#73499PurposeConstitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locusDepositorInsertdCas9-KRAB
UseCRISPRTagsHA, KRAB, and NLSExpressionMammalianMutationD10A, H840A (catalytically deactivated Cas9)PromoterCAGAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3 1 kb prom. + Enhancer CD274
Plasmid#107005PurposeModified Luciferase expression vectorDepositorInsertCD274 (CD274 Human)
UseLuciferaseTagsExpressionMammalianMutationN.B. There are two published SNPs in this region …PromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ Myc2-mouse TTP aa S52 S178 MUT
Plasmid#107001PurposeExpresses epitope-tagged double-mutant murine TTPDepositorInserttristetraprolin (Zfp36 Mouse)
UseTagsDouble Myc peptide epitope tagExpressionMammalianMutationChanged Ser52 to Ala and Ser178 to AlaPromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH-TAZ S89A
Plasmid#179910PurposeConstruct for expressing an activated form of TAZ (encoding be gene WWTR1)DepositorInsertWW Domain Containing Transcription Regulator 1 (WWTR1 Human)
UseTags3X Flag-tagExpressionMammalianMutationSerine 89 mutated to AlaninePromoterEF1aAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hygro-2xMyc-Rac1-WT
Plasmid#128580PurposeFor mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.DepositorInsertRac1 (Rac1 Mouse)
UseRetroviralTags2xMyc epitopeExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final
Plasmid#107009PurposeModified Luciferase expression vectorDepositorInsertCD274 (CD274 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanCOL2A1enhancer
Plasmid#173961PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertEnhancer region from COL2A1 gene
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX458-sgTSC2
Plasmid#128098PurposeCRISPR gRNA against human TSC2 with Cas9 from S. pyogenes and 2A-EGFPDepositorInsertsgRNA against human TSC2 (TSC2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45
Plasmid#173946PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertSOX9 enhancer cluster EC1.45
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3 3'UTR reporter MUT 1.3 kb CD274 Hs 3'UTR Final
Plasmid#107010PurposeModified Luciferase expression vectorDepositorInsertCD274 (CD274 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
mApple-Actin-C-18
Plasmid#54863PurposeLocalization: Actin, Excitation: 568, Emission: 592DepositorInsertActin (ACTB Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-ALG13 (OTU, aa 216-353)
Plasmid#61414PurposeExpresses human ALG13 (OTU domain) in E. coli.DepositorInsertALG13 (Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13) (ALG13 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-215 and aa 354-1137.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRP-B-catenin-V5-LgBiT
Plasmid#241816PurposeExpresses V5-tagged and LgBiT-tagged B-catenin for use with the Promega HiBiT systemDepositorInsertB-catenin
UseTagsLgBiT and V5ExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NDi-CRISPRi (Gen1)
Plasmid#73497PurposeDox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry markerDepositorInsertsdCas9-KRAB-P2A-mCherry
rtTA
UseCRISPRTagsHA, KRAB, and NLSExpressionMammalianMutationD10A, H840A (catalytically deactivated Cas9)PromoterCAG and TRE3GAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NDi-CRISPRi (Gen2)
Plasmid#73498PurposeDox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locusDepositorInsertsdCas9-KRAB
rtTA
UseCRISPRTagsHA, KRAB, and NLSExpressionMammalianMutationD10A, H840A (catalytically deactivated Cas9)PromoterCAG and TRE3GAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1181 - pmU6(loxP-STOP-loxP) BbsI gRNA
Plasmid#113160PurposeA plasmid for cloning Cre-dependent guide RNAs using a modified mouse U6 promoter containing loxPDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromotermU6Available sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IP-mCherry-TOSI
Plasmid#172496PurposemCherry-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.DepositorInsertPDCD4 (Pdcd4 Mouse)
UseRetroviralTagsMcherryExpressionMammalianMutationPromoterMLV LTRAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IP-mVenus-TOSI
Plasmid#172491PurposemVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.DepositorInsertPdcd4 (Pdcd4 Mouse)
UseRetroviralTagsmVenusExpressionMammalianMutationPromoterMLV LTRAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hygro-2xMyc-Rac1-P29S
Plasmid#128581PurposeFor mammalian cell expression of P29S mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.DepositorInsertRac1 (Rac1 Mouse)
UseRetroviralTags2xMyc epitopeExpressionMammalianMutationChanged Proline29 to Serine.PromoterAvailable sinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_min1-2
Plasmid#173952PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertMinimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.25_S3-4-5-6
Plasmid#173956PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertEnhancer regions 3-6 (S3, S4, S5 and S6) from SOX9 enhancer cluster EC1.25
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZ194
Plasmid#163640Purposerps-27>DHB::2xmKate2-P2A-H2B::GFP expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome IDepositorInsertDHB::2mKate2-P2A-H2B::GFP::3xHA (his-58 Nematode, Synthetic)
UseCRISPRTags2xmKate2 and GFPExpressionWormMutationPromoterrps-27Available sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hygro-2xMyc-Rac1-Q61L
Plasmid#128582PurposeFor mammalian cell expression of Q61L mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.DepositorInsertRac1 (Rac1 Mouse)
UseRetroviralTags2xMyc epitopeExpressionMammalianMutationChanged Glutamine61 to Leucine.PromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAWK61
Plasmid#163642Purposerps-27>DHB::GFP-P2A-H2B::2xmKate2 expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome IDepositorInsertDHB::GFP-P2A-H2B::2xmKate2::3xHA (his-58 Human, Nematode)
UseCRISPRTags2xmKate2 and GFPExpressionWormMutationPromoterrps-27Available sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1
Plasmid#173947PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 from SOX9 enhancer cluster EC1.45
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-mouseEC1.45
Plasmid#173972PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertMouse orthologous SOX9 enhancer cluster EC1.45
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.25_S6
Plasmid#173960PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertEnhancer region 6 (S6) from SOX9 enhancer cluster EC1.25
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.35_S1
Plasmid#173954PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertEnhancer region 1 (S1) from SOX9 enhancer cluster EC1.35
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.35_S1-2
Plasmid#173953PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertEnhancer regions 1 and 2 (S1 and S2) from SOX9 enhancer cluster EC1.35
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1-2_del12
Plasmid#173984PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #12
UseLuciferaseTagsExpressionMutationTiled deletion #12PromoterSV40 promoterAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.25_S5
Plasmid#173959PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertEnhancer region 5 (S5) from SOX9 enhancer cluster EC1.25
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.25_S4
Plasmid#173958PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertEnhancer region 4 (S4) from SOX9 enhancer cluster EC1.25
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak2
Plasmid#173948PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 2 from SOX9 enhancer cluster EC1.45
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1-2_del3
Plasmid#173975PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #3
UseLuciferaseTagsExpressionMutationTiled deletion #3PromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1-2_del6
Plasmid#173978PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #6
UseLuciferaseTagsExpressionMutationTiled deletion #6PromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1-2_del4
Plasmid#173976PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #4
UseLuciferaseTagsExpressionMutationTiled deletion #4PromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1-2_del1
Plasmid#173973PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #1
UseLuciferaseTagsExpressionMutationTiled deletion #1PromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1-2_del10
Plasmid#173982PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #10
UseLuciferaseTagsExpressionMutationTiled deletion #10PromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1-2_del9
Plasmid#173981PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #9
UseLuciferaseTagsExpressionMutationTiled deletion #9PromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1-2_del11
Plasmid#173983PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #11
UseLuciferaseTagsExpressionMutationTiled deletion #11PromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_min2
Plasmid#173951PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertMinimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1-2_del2
Plasmid#173974PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #2
UseLuciferaseTagsExpressionMutationTiled deletion #2PromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_min1
Plasmid#173950PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertMinimal enhancer region 1 (min1) from SOX9 enhancer cluster EC1.45
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1-2_del5
Plasmid#173977PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #5
UseLuciferaseTagsExpressionMutationTiled deletion #5PromoterSV40 promoterAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1-2
Plasmid#173949PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.35_S2
Plasmid#173955PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertEnhancer region 2 (S2) from EC1.35 SOX9 enhancer cluster EC1.35
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1-2_del7
Plasmid#173979PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #7
UseLuciferaseTagsExpressionMutationTiled deletion #7PromoterSV40 promoterAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only