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pLKO.1 puro shRNA beta-catenin

Plasmid

#18803

Purpose

3rd gen lentiviral vector for knocking down beta-catenin gene expression

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

b-catenin shRNA (CTNNB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

July 22, 2008

Availability

Academic Institutions and Nonprofits only

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pC1-HyPer-3

Plasmid

#42131

Depositor

Vsevolod Belousov

Article

Bilan et al ACS Chem Biol. 2012 Dec 20.

Insert

HYPER 3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCMVHA SUZ12

Plasmid

#24232

Purpose

Mammalian expression of human SUZ12 with HA tag

Depositor

Kristian Helin

Article

Pasini et al EMBO J. 2004 Oct 13. 23(20):4061-71.

Insert

SUZ12 (SUZ12 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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ES-FUCCI

Plasmid

#62451

Purpose

SMX_DEST vector with 3 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
minPGK::Cherry-hCdt1(30-120)-bGHpA
PGK::HygroR-bGpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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EZ-Tet-pLKO-Hygro

Plasmid

#85972

Purpose

Tet/Dox inducible shRNA lentivirus with hygromycin selection

Depositor

Cindy Miranti

Article

Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Promoter

H1 / TetO

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-mTwist

Plasmid

#1783

Depositor

Bob Weinberg

Article

Yang et al Cell 2004 Jun 25;117(7):927-39.

Insert

Twist (Twist1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 10, 2005

Availability

Academic Institutions and Nonprofits only

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pMIG-FLAG-MLL-AF9

Plasmid

#71443

Purpose

Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFP

Depositor

Daisuke Nakada

Article

Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

Insert

MLL-Af9 (KMT2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

MSCV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-Ku80

Plasmid

#46958

Purpose

Plasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

Ku80 (XRCC5 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pLJC5-Tmem192-2xFlag

Plasmid

#102929

Purpose

Lentiviral expression of a lysosomal tag (Tmem192-Flag)

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

Tmem192 (TMEM192 Human)

Use

Lentiviral

Tags

Flag

Promoter

ubc

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-JMJD2A

Plasmid

#24180

Purpose

Mammalian expression of human JMJD2A with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

JMJD2A (KDM4A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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CMV-GEM-GECO1

Plasmid

#32442

Purpose

Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

GEM-GECO1

Expression

Mammalian

Mutation

GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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Ube1/PET21d

Plasmid

#34965

Depositor

Cynthia Wolberger

Article

Berndsen et al Anal Biochem. 2011 Nov 1;418(1):102-10.

Insert

Ube1 (UBA1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pGreenIIM DR5v2-ntdTomato/DR5-n3GFP

Plasmid

#61628

Purpose

Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter

Depositor

Dolf Weijers

Article

Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

Inserts

ntdTomato
n2xEGFP

Expression

Plant

Mutation

2 copies of EGFP with N-terminal SV40 NLS

Promoter

Auxin-dependent DR5 promoter containing 9 reverse…

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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LZRS-HOTAIR

Plasmid

#26110

Depositor

Howard Chang

Article

Gupta et al Nature. 2010 Apr 15. 464(7291):1071-6.

Insert

HOTAIR (HOTAIR Human)

Use

Retroviral

Available Since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Myc-DNMT3B1

Plasmid

#35522

Purpose

Mammalian expression of human DNMT3B1 with myc tag

Depositor

Arthur Riggs

Article

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Insert

DNMT3B1 (DNMT3B Human)

Tags

Myc

Expression

Mammalian

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only

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CMV-G-GECO1.2

Plasmid

#32446

Purpose

Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2)

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.2

Expression

Mammalian

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-UTX

Plasmid

#24168

Purpose

Mammalian expression of human UTX with HA tag

Depositor

Kristian Helin

Article

Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

Insert

UTX (KDM6A Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-Ku70

Plasmid

#46957

Purpose

Plasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

Ku70 (XRCC6 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pCAGGS-flpE-puro

Plasmid

#20733

Purpose

Mammalian expression of FLPe recombinase from the highly-expressed CAGGS promoter; puro selectable marker

Depositor

Rudolf Jaenisch

Article

Beard et al Genesis. 2006 Jan . 44(1):23-8.

Insert

FLPe recombinase

Use

Flp/frt

Expression

Mammalian

Promoter

CAGGS

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2-UTX-F

Plasmid

#17438

Purpose

Expression of UTX in mammalian cells

Depositor

Kai Ge

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

Insert

UTX (KDM6A Human)

Tags

6xMyc and Flag

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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