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Showing: 201 - 220 of 5986 results

  7. p425_Cas9_gRNA_LEU_1014a

    Plasmid
    #87407
    Purpose
    p425_Cas9_gRNA-ARS1014a All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, LEU2 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1014a sequence TTATGTGCGTATTGCTTTCA in yeast chromosome 10.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1014a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87407-plasmid-map-sequence-id-193449

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  8. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid
    #174140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174140-plasmid-map-sequence-id-341918

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  9. dCas9-miniTurbo

    Plasmid
    #219822
    Purpose
    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)
    Depositor
    Philipp Maass
    Insert
    dCas9
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    EF1a
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219822-plasmid-map-sequence-id-440858

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  10. pCas9_GFP

    Plasmid
    #44719
    Purpose
    Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Article
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Insert
    Cas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 24, 2013
    Availability
    Academic Institutions and Nonprofits only
    44719-plasmid-map-sequence-id-372914

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  11. pZ8-T_dCas9

    Plasmid
    #74062
    Purpose
    pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    dcas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ptac
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    74062-plasmid-map-sequence-id-137972

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  12. eSpCas9(1.1)

    Plasmid
    #71814
    Purpose
    Expresses high specificity SpCas9. Px330-like plasmid.
    Depositor
    Feng Zhang
    Article
    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.
    Has Service
    Cloning Grade DNA
    Insert
    enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71814-plasmid-map-sequence-id-177023

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  13. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

    Plasmid
    #42335
    Purpose
    A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.
    Depositor
    Feng Zhang
    Article
    Cong et al Science. 2013 Jan 3.
    Has Service
    Cloning Grade DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Promoter
    Available since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    42335-plasmid-map-sequence-id-275689

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  16. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid
    #174138
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174138-plasmid-map-sequence-id-341915

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  17. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

    Plasmid
    #174139
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174139-plasmid-map-sequence-id-341913

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  18. pCW-Cas9-Blast

    Plasmid
    #83481
    Purpose
    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA
    Depositor
    Mohan Babu
    Inserts
    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    Tight Tre and human phosphoglycerate kinase 1 pro…
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83481-plasmid-map-sequence-id-335118

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  19. mPLD3-sgRNA-Cas9-mcherry

    Plasmid
    #199700
    Purpose
    encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199700-plasmid-map-sequence-id-390617

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Showing: 201 - 220 of 5986 results