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pHR-H13LTat-Thy1.2-P2A-GFP-T2A-Nef

Plasmid

#126553

Purpose

Replication incompetent HIV with H13L Tat and GFP-t2a-Thy1.2 reporter

Depositor

Jonathan Karn

Article

Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

Insert

HIV-1 vector pNL4-3

Use

Lentiviral

Tags

Thy1.2 and GFP

Promoter

HIV LTR

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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Psy074

Plasmid

#99504

Purpose

Bacterial expression of LIPS M1+TE

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

LIPS M1+TE

Expression

Bacterial

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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MAP2-GCaMP3

Plasmid

#43917

Depositor

John Gearhart

Article

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Insert

GCaMP3

Use

Lentiviral

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

E247V in GCaMP3

Promoter

Human MAP2 promoter

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-hCAS-pA

Plasmid

#48625

Purpose

Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii, Kunihiko Naito

Article

Fujii et al Nucleic Acids Res. 2013 Aug 30.

Insert

Codon optimized Cas9

Use

CRISPR

Tags

FLAG, NLS, and polyA

Expression

Mammalian

Promoter

CAG promoter

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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GFAP-Cre

Plasmid

#24704

Depositor

Michael Sofroniew

Article

Bush et al Neuron. 1999 Jun . 23(2):297-308.

Insert

Cre

Expression

Mammalian

Promoter

GFAP (glial fibrillary acidic protein)

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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Ki67∆FHA-mNeonGreen-IRESpuro2

Plasmid

#183739

Purpose

Expresses Ki67∆FHA-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67_lacking FHA (MKI67 Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

MKI67(lacking FHA)

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pET30a-GlmU-NahK-ET64

Plasmid

#234663

Purpose

Expression an ELP-tagged fusion enzyme of GlmU and NahK

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

N-acetylglucosamine- 1-phosphate uridyltransferase
N-acetylhexosamine 1-kinase

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ConVERGD-ConFonvCon-eGFP-W3SL

Plasmid

#218752

Purpose

Provides AND intersectional (Cre+Flp+vCre) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Promoter

hSyn

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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p416 25Q GPD

Plasmid

#1177

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 25Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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p416 103Q GPD

Plasmid

#1180

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 103Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pCk4_spacer

Plasmid

#136074

Purpose

Use when making even level constructs (L2,...) if pCk4 position (position 4) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hSyn-ConVERGD-TVA(mChr)-W3SL

Plasmid

#218754

Purpose

Provides AND intersectional (Cre+Flp) expression of TVA-mCherry

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

TVA-mCherry

Use

AAV

Tags

mCherry

Promoter

hSyn

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pTK14

Plasmid

#37360

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

LGN (GPSM2 Human)

Use

Retroviral

Tags

GFP, S-tag, and TEV

Expression

Mammalian

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ConVERGD-ArchT(eGFP)-WPRE

Plasmid

#218760

Purpose

Provides AND intersectional (Cre+Flp) expression of ArchT-eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

ArchT

Use

AAV

Tags

eGFP

Promoter

hSyn

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCk2

Plasmid

#136696

Purpose

Acceptor plasmid for odd level position 2, lacZ blue-white screening.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZH82 pET28a-Rai1

Plasmid

#231651

Purpose

Bacterial expression vector expressing yeast S. cerevisiae rai1, no tag

Depositor

Jesper Svejstrup

Article

Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

Insert

rai1 (RAI1 Budding Yeast)

Expression

Bacterial

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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p426 103Q GAL

Plasmid

#1188

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 103Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
NylCK_pET28a

Plasmid

#215422

Purpose

Expression construct for NylCK gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCK

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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M-NylC_pET28a

Plasmid

#215409

Purpose

Expression construct for M-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

M-NylC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBP-J23100

Plasmid

#72963

Purpose

Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

J23100 promoter

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Myc-CICf

Plasmid

#48185

Purpose

Expresses Cic

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Capicua (Cic Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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p416 72Q GPD

Plasmid

#1179

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 72Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
pBetaActin-TUBB2a-mEos3.2-mEos3.2-mEos3.2

Plasmid

#191338

Purpose

Expresses triple fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light in slice cultures

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mEos3.2-mEos3.2-mEos3.2 (Tubb2a Mouse)

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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HSP70-pp7i-mCh-UPG

Plasmid

#161011

Purpose

C-terminal mCherry fusion to Arabidopsis HSP70 with intronic PP7

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-mMALAT1_3' WT Sense

Plasmid

#46834

Purpose

Express cGFP transcript ending in the wildtype MALAT1 triple helix

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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RP185 pUAST EGFP:Or47b

Plasmid

#59525

Purpose

N-terminal fusion of GFP to Drosophila melanogaster Or47b

Depositor

Leslie Vosshall

Article

Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

Insert

EGFP:Or47b (Or47b Fly)

Tags

EGFP

Expression

Insect

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pCk1

Plasmid

#136695

Purpose

Acceptor plasmid for odd level position 1, lacZ blue-white screening.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEx-insertion-erm

Plasmid

#172210

Purpose

For genetic insertion in erythromycin-sensitive Prevotella copri strains

Depositor

Till Strowig

Article

Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

Type

Empty backbone

Use

Genetic insertion in prevotella copri

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pPM1003

Plasmid

#167550

Purpose

Encodes genes that confer expression of Vibrio cholerae O antigens of the Ogawa serotype in E. coli K-12

Depositor

Renato Morona

Article

Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

Insert

Vibrio cholerae O antigens genes of the Ogawa serotype

Use

Cosmid

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pBP-lacZ

Plasmid

#72948

Purpose

Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 0 - Bioparts

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCk4

Plasmid

#136698

Purpose

Acceptor plasmid for odd level position 4, lacZ blue-white screening.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFW6000

Plasmid

#224215

Purpose

Bacteroides genomic editing tool; aTc inducible-Fncpf1

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Insert

Fncpf1

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Lepr peak reporter-eGFP

Plasmid

#196584

Purpose

Cis-regulatory region of mouse Lepr gene driven a eGFP reporter with hGFP driven rtTA3

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

eGFP

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pUAP4

Plasmid

#136079

Purpose

Acceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, lacZ blue-white screening. Release parts to assemble transcription units with BsaI.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGEMT-PT2A-GFP-Tdtomato-iRFP670

Plasmid

#111812

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

GFP
Tdtomato
iRFP670

Use

Cloning intermediate

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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FU-MAP2-Gateway

Plasmid

#43915

Depositor

John Gearhart

Article

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Tags

V5 and non-standard His tag (HHRHHH)

Expression

Mammalian

Promoter

Human MAP2 promoter

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pYC3.60-C1

Plasmid

#67899

Purpose

Yellow Cameleon 3.60 in a clontech-style backbone for expression in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

YC3.60

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only
GFAP-Arclight

Plasmid

#187978

Purpose

Expresses the fluorescent voltage indicator Arclight under the GFAP promoter

Depositor

Chris Dulla

Article

Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

Insert

Arclight A242

Use

AAV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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Ki67∆Nterm-mNeonGreen-IRESpuro2

Plasmid

#183740

Purpose

Expresses Ki67∆Nterm-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 LR domain + all Ki-67 repeats (MKI67 Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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H2B-2A-GFP-IRESpuro2

Plasmid

#183746

Purpose

Expresses H2B-2A-GFP

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

H2B (H2BC16P Human)

Tags

GFP with 2A protease cleavage site between H2B an…

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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