We narrowed to 62,948 results for: CaS;

1. pX459-HypaCas9-H2BC11_sgRNA

   Plasmid

   #183883

   Purpose

   pX459V2.0-HypaCas9 plasmid with H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   H2BC11 sgRNA spacer (H2BC11 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459-HypaCas9-MYH10_sgRNA

   Plasmid

   #183887

   Purpose

   pX459V2.0-HypaCas9 plasmid with MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   MYH10 sgRNA spacer (MYH10 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX459-HypaCas9-CfANLN_sgRNA

   Plasmid

   #183880

   Purpose

   pX459V2.0-HypaCas9 plasmid with cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   Canis familiaris ANLN sgRNA spacer
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJCC_103 SpCas9 T1337C

   Plasmid

   #179525

   Purpose

   For bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 T1337C
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed threonine 1337 to cysteine

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1137B=Hsp70Bb-Cas9

   Plasmid

   #153284

   Purpose

   The attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Insert

   pHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)
   

   Use

   CRISPR

   Tags

   eGFP

   Expression

   Insect

   Mutation

   Promoter

   Dmel Hsp70Bb

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCASCADE-gltA1-gltA2

   Plasmid

   #71348

   Purpose

   gltA1-gltA2 targeting guide RNA E. coli , Low Phosphate Induction

   Depositor

   Michael Lynch

   Article

   Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

   Insert

   gltA1-gltA2 guide array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E . coli ugpB gene promoter, low phosphate induct…

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCASCADE-udhA-xylA

   Plasmid

   #158612

   Purpose

   Low phosphate inducible gRNA array to silence the xylA and udhA promoters

   Depositor

   Michael Lynch

   Article

   Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

   Insert

   udhA-xylA guide array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E . coli ugpB gene promoter, low phosphate induct…

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCASCADE-gltA2-xylA

   Plasmid

   #158613

   Purpose

   Low phosphate inducible gRNA array to silence the xylA and gltA2 promoters

   Depositor

   Michael Lynch

   Article

   Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

   Insert

   gltA2-xylA guide array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E . coli ugpB gene promoter, low phosphate induct…

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCASCADE-zwf-xylA

   Plasmid

   #158614

   Purpose

   Low phosphate inducible gRNA array to silence the xylA and zwf promoters

   Depositor

   Michael Lynch

   Article

   Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

   Insert

   zwf-xylA guide array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E . coli ugpB gene promoter, low phosphate induct…

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 6xHis-MBP-huMlCas12a

    Plasmid

    #174678

    Purpose

    Expresses 6xHis-MBP tagged MlCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huMlCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330 C-FKBP-Cas9

    Plasmid

    #162729

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. L2_NOP1gRNA-Cas9-CsA

    Plasmid

    #136139

    Purpose

    Plasmid with NOP1-gRNA to tranform Mp as CRISPR control. Contains Cas9 and HygR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35Sx2:HygR p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. L2_2xNOP1gRNA-Cas9-CsA

    Plasmid

    #136140

    Purpose

    Plasmid with 2 different NOP1-gRNA. Targets 500 bp apart in the genome. To tranform Mp as CRISPR control. Contains Cas9 and HygR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35S:HygR p5-MpU6:NOP1gRNA2 p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNOC_dfnCas12a-KRAB_crRNA Td2

    Plasmid

    #176261

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    Kruppel associated box domain and luciferase

    Expression

    Mutation

    D917A and E1006A mutations to inactivate the endo…

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNOC_hfnCas12a-Nlux_crRNA NR2

    Plasmid

    #176245

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNOC_hfnCas12a-Nlux_crRNA NR3

    Plasmid

    #176246

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 6xHis-MBP-huCMaCas12a

    Plasmid

    #174679

    Purpose

    Expresses 6xHis-MBP tagged CMaCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huCMaCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 6xHis-MBP-huPxCas12a

    Plasmid

    #174675

    Purpose

    Expresses 6xHis-MBP tagged PxCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPxCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 6xHis-MBP-huPrCas12a

    Plasmid

    #174674

    Purpose

    Expresses 6xHis-MBP tagged PrCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPrCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 6xHis-MBP-huBfCas12a

    Plasmid

    #174672

    Purpose

    Expresses 6xHis-MBP tagged BfCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huBfCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge