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pT3-EF1aH Yap S127A

Plasmid

#86497

Purpose

Mammalian expression of FLAG-tagged Yap containing S127A mutation

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Yes associated protein 1 (YAP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S127A

Promoter

EF1a

Available Since

Feb. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pSMP-EZH2_1

Plasmid

#36387

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

EZH2 (EZH2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc-NLRP3(R260W)

Plasmid

#73956

Purpose

Express c-Myc-tagged human NLRP3(R260W) in mammalian cells

Depositor

Christian Stehlik

Article

Dorfleutner et al Infect Immun. 2007 Mar;75(3):1484-92. Ep

Insert

NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)

Tags

c-myc

Expression

Mammalian

Mutation

R260W

Promoter

CMV iE

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only
pCI-neo-RL-ZEB1

Plasmid

#35535

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

3'UTR of ZEB1 (ZEB1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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FCHo1-pmCherryC1

Plasmid

#27690

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

FCHo1 (Fcho1 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
pEGFPC1-FLAG-Synaptojanin 1-170

Plasmid

#22294

Depositor

Pietro De Camilli

Article

Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

Insert

Synaptojanin 1_170 (SYNJ1 Human)

Tags

Flag and GFP

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pLX304-YAP1(S94A)

Plasmid

#59145

Purpose

YAP1 serine 94 o alanine mutants which disrupts binding to TEAD transcription factors

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

S94A

Promoter

CMV

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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cfSGFP2-N

Plasmid

#37533

Depositor

Ikuo Wada

Article

Suzuki et al PLoS One. 2012;7(5):e37551. Epub 2012 Ma

Insert

cysteine-free SGFP2-N

Expression

Mammalian

Mutation

C48S and C70M mutations relative to SGFP2

Promoter

CMV

Available Since

Aug. 6, 2012

Availability

Academic Institutions and Nonprofits only

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hTRAF2pLPCX-HA-Flag/P874

Plasmid

#20229

Depositor

Martin Dorf

Article

Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

Insert

TNF receptor-associated factor 2 (TRAF2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

unmutated NM_021138.3. L replaces M at beginning…

Available Since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

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PECAM-eGFP

Plasmid

#14688

Depositor

Victoria Bautch

Article

Kearney et al Blood. 2004 Jun 15. 103(12):4527-35.

Insert

PECAM promoter/enhancer (Pecam1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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GFP-PIPK1 gamma 87

Plasmid

#22300

Depositor

Pietro De Camilli

Article

Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

Insert

human phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pCR-HA-IKKbeta

Plasmid

#15470

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase beta (Ikbkb Mouse)

Tags

HA

Expression

Mammalian

Available Since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only
3341 pcDNA3 Bcl-2 T69A S70A S87A

Plasmid

#13340

Depositor

Stanley Korsmeyer

Article

Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

Insert

Bcl-2 (BCL2 Human)

Expression

Mammalian

Mutation

changed Threonine 69 to Alanine; changed Serine 7…

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only
pCMV5 WT KSR1

Plasmid

#25970

Depositor

Rob Lewis

Article

Joneson et al J Biol Chem. 1998 Mar 27. 273(13):7743-8

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-Tuba

Plasmid

#22214

Depositor

Pietro De Camilli

Article

Salazar et al J Biol Chem. 2003 Dec 5. 278(49):49031-4

Insert

Tuba (DNMBP Human)

Tags

HA

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only
myc-mTOR (1271-2008 aa)

Plasmid

#21746

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1271-2008) (Mtor Rat)

Tags

myc

Expression

Mammalian

Mutation

amino acids 1271-2008

Available Since

Aug. 18, 2009

Availability

Academic Institutions and Nonprofits only
myc-mTOR (1750-2549 aa)

Plasmid

#21747

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1750-2549) (Mtor Rat)

Tags

myc

Expression

Mammalian

Mutation

amino acids 1750-2549

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pCR-Flag-IKKbeta-KM

Plasmid

#15466

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase beta-KM (Ikbkb Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed Lysine at 44 to Alanine

Available Since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only
pLV hUbC-dCas9-MQ1(Q147L)-EGFP

Plasmid

#89793

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR and Lentiviral

Tags

3*FLAG and T2A-EGFP

Expression

Mammalian

Mutation

Q147L, CAA-->CTA

Promoter

hUbC

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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DNMT3A-dCas9

Plasmid

#100090

Purpose

DNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Insert

DNMT3A (DNMT3A Human)

Use

CRISPR

Tags

3XFLag-NLS-DNMT3A-dCas9-NLS

Expression

Mammalian

Mutation

includes aa 602-912

Promoter

CMV

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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