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pFB1_hMSH6

Plasmid

#129424

Purpose

Expression human MSH6 protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MSH6 (MSH6 Human)

Expression

Insect

Promoter

Polyhedrin promoter

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSMP-YY1_2

Plasmid

#36358

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

YY1 (YY1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
th-P2A-Gal4 donor

Plasmid

#65563

Purpose

Intron targeting-mediated Gal4 knockin at the zebrafish th locus.

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

th-P2A-Gal4 (th Zebrafish)

Use

Zebrafish injection

Promoter

none

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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RabV CVS-N2c(deltaG)-mCherry

Plasmid

#73464

Purpose

Expresses mCherry for monosynaptic circuit mapping

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

mCherry

Use

Neurotropic virus

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pFGL1252_TetGFP(Hyg)

Plasmid

#118993

Purpose

TetOFF controlled GFP-tagging vector with Hygromycin selection

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Insert

eGFP

Use

Fungal expression (in magnaporthe oryzae)

Promoter

Ustilago maydis Mfa1 basal promoter with six tetO…

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-empty

Plasmid

#105594

Purpose

empty control vector with GFP marker and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

no insert, empty control vector

Use

Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pDA019

Plasmid

#131452

Purpose

control expression vector

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Type

Empty backbone

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pICSL90003

Plasmid

#68262

Purpose

Level 0 Golden Gate Part: Promoter U6 (Triticum aestivum)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter: U6 (Triticum aestivum)

Use

Synthetic Biology

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-LacI

Plasmid

#183920

Purpose

Lac repressor labeled with GFP; can be used to label lacO sites

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

GFP-LacI (lacI Synthetic)

Expression

Mammalian

Mutation

none

Promoter

SV2

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pFB1_hPMS2

Plasmid

#129425

Purpose

Expression human PMS2 protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human PMS2 (PMS2 Human)

Expression

Insect

Promoter

Polyhedrin promoter

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pEx-deletion-erm

Plasmid

#172213

Purpose

For allelic exchange in erythromycin-sensitive Prevotella copri strains

Depositor

Till Strowig

Article

Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

Type

Empty backbone

Use

Allelic exchange in prevotella copri

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV CAGG eGFP

Plasmid

#107707

Purpose

express eGFP in variety of cell types

Depositor

Troy Margrie

Article

Velez-Fort et al Neuron. 2018 Apr 4;98(1):179-191.e6. doi

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

CAGG

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-TaGu-WT-3xF-ORF

Plasmid

#238487

Purpose

For production of C-term flag-tagged TaGu R2 ORF mRNA for mRNA transfection in PRINT

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement mRNA

Use

Ivt template

Tags

3x-Flag

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-YFP-PKD1

Plasmid

#31527

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2009 Sep 4. 284(36):24653-6

Insert

YFP-HA-Protein Kinase D (PRKD1 Human)

Tags

HA and YFP

Expression

Mammalian

Available Since

Nov. 3, 2011

Availability

Academic Institutions and Nonprofits only
pHW522 (Prab-3::cGAL-C::let-858 3'UTR)

Plasmid

#107130

Purpose

cGAL-C driver under the control of C. elegans rab-3 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::gp41-1-C-intein::cGAL(AD)

Expression

Worm

Promoter

C. elegans rab-3 promoter

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMW37: pRS426-R2Bm RNA

Plasmid

#209291

Purpose

Contains the R2Bm non-LTR retrotransposon 5'UTR and 3'UTR sequences

Depositor

Feng Zhang

Article

Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

Insert

R2Bm 5'UTR and 3'UTR

Expression

Bacterial and Yeast

Promoter

GAL1 GAPDH

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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MSGV Mouse Acceptor

Plasmid

#128176

Purpose

A TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR ref Biotechniques. 2015 Mar 1;58(3):135-9

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Promoter

MSCV

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pKEK3238

Plasmid

#234092

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

sfCherry2

Expression

Bacterial

Mutation

E117Q/T127I/G219A

Promoter

pJ23100-BsaI

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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plk1 T210A

Plasmid

#132965

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Article

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1 T210A (PLK1 Human)

Expression

Mammalian

Mutation

T210A

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAL101

Plasmid

#22474

Purpose

Expresses APB domain of PIF6 fused to mYFP, for red-light-induced binding to Phy

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PIF6APB

Use

Synthetic Biology

Tags

mYFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) FLIPQTV3.0 100micro

Plasmid

#63730

Purpose

FLIPQTV3.0 glutamine sensor with D157N mutation

Depositor

Sakiko Okumoto

Article

Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

Insert

FLIPQTV3.0 sensor, 100microM affinity

Expression

Mammalian

Mutation

D157N in glutamine binding domain

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-F30-Tornado-Acyclovir-Broccoli#4

Plasmid

#216822

Purpose

Expresses acyclovir-controllable Broccoli aptamer #4 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-Broccoli#4

Expression

Mammalian

Promoter

U6+27

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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tCRMod-GGSGGS5

Plasmid

#111760

Purpose

Expresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domain

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-GGSGGS5-mRuby2 fusion

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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optoAKT

Plasmid

#207164

Purpose

Light-inducible expression of optoAKT; for precise temporal control of the AKT signaling pathway

Depositor

Kai Zhang

Article

Wang et al Elife. 2020 Oct 6;9:e57395. doi: 10.7554

Insert

CRY2-mCherry-AKT(_PH)-2A-CIBN2-GFP-CAAX

Expression

Mammalian

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pKEK3233

Plasmid

#234105

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

YukonOFP

Expression

Bacterial

Promoter

pJ23100-BsaI

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL90002

Plasmid

#68261

Purpose

Level 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter: U6-26 (Arabidopsis thaliana)

Use

Synthetic Biology

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-TetR-KRAB-IRES-BLAS

Plasmid

#55820

Purpose

retroviral bicistronic expression vector for TetR-KRAB and Blasticidin S resistance

Depositor

Stephan Geley

Article

Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

Insert

TetR-KRAB

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pcDN3.1(+)-CaNARi

Plasmid

#64848

Purpose

FRET-based calcineurin (CaN) activation biosensor.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNARi

Tags

Cerulean and Venus

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCk2

Plasmid

#136696

Purpose

Acceptor plasmid for odd level position 2, lacZ blue-white screening.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-TaGu-ENT-3xF-ORF

Plasmid

#238488

Purpose

For production of C-term flag-tagged TaGu EN-tuned ORF mRNA for mRNA transfection in PRINT

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement mRNA

Use

Ivt template

Tags

3x-Flag

Mutation

R1109->A

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL33

Plasmid

#227430

Purpose

Kanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)

Depositor

Pierre Santucci

Article

Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:

Insert

mWasabi

Use

CRISPR

Expression

Bacterial

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL34

Plasmid

#227431

Purpose

Kanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)

Depositor

Pierre Santucci

Article

Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:

Insert

tdTomato

Use

CRISPR

Expression

Bacterial

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
tdTomato-LacI

Plasmid

#183921

Purpose

Lac repressor labeled with tdTomato; can be used to label lacO sites

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdTomato-LacI (lacI Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVTH-sip53

Plasmid

#12239

Depositor

Didier Trono

Article

Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

Insert

shRNA against p53 (TP53 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
RabV CVS-N2c(deltaG)-FlpO

Plasmid

#73473

Purpose

Expresses FlpO for circuit mapping and genetic manipulation

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

FlpO

Use

Neurotropic virus

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pORF1-2

Plasmid

#206024

Purpose

An expression vector for mouse LINE1 ORF1-2

Depositor

Masamichi Kamihira

Article

Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

Insert

ORF1-2 derived from mouse LINE1

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ER50-SpCas9-ER50

Plasmid

#85448

Purpose

Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

ER50-SpCas9-ER50

Use

AAV

Tags

destabilized domain of estrogen receptor ligand b…

Expression

Mammalian

Promoter

Cbh

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-DAG1

Plasmid

#205149

Purpose

Overexpress DAG1

Depositor

Monkol Lek

Insert

Dystroglycan 1 (DAG1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Carries common missense variant c.41C>G (p.Ser…

Promoter

EF-1a

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pOVC1_ScPH1-S_X

Plasmid

#131184

Purpose

Expresses ScPH1-S (Synechosystis PCC6803) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

ScPH1-S

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpyC-GFP

Plasmid

#220191

Purpose

sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG42 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG42

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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