We narrowed to 18,718 results for: IRE

1. pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST

   Plasmid

   #127332

   Purpose

   Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRES

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and CL1/PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-EF1alpha-SARS-CoV-2-nsp11-2xStrep-IRES-Puro

   Plasmid

   #141377

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-nsp11 (ORF1ab SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available since

   April 9, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1

   Plasmid

   #138417

   Purpose

   CAPTURE1.1 vector containing BirA-V5-His, P2A, FB-dCas9, IRES and zsGreen1

   Depositor

   Jian Xu

   Article

   Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

   Inserts

   BirA
   dCas9
   

   Use

   Lentiviral

   Tags

   FLAG, Avi-tag and V5, His

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR- GAL4-UAS-BAMH1-IRES-mCherry-PGK-BFP

   Plasmid

   #223215

   Purpose

   Lentiviral vector - mCherry reporter with a BAMH1 site for Gal4DBDVP64 synNotch receptors with a constitutive BFP

   Depositor

   Wendell Lim

   Article

   Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

   Insert

   mCherry BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-GAL4-UAS-activemTGFb1-IRES-mCherry-PGK-BFP

   Plasmid

   #223213

   Purpose

   Lentiviral vector - mCherry reporter and TGFb1 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

   Depositor

   Wendell Lim

   Article

   Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

   Insert

   TGFb1 active - membrane (TGFB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE mNeonGreen-Core (HBc from HBV) IRES puro

   Plasmid

   #122202

   Purpose

   Plasmid coding for mNeonGreen-Core protein (from HBV AYW) and a puromycin resistance in a lentiviral backbone.

   Depositor

   Raphael Gaudin

   Article

   Hallez et al Nat Microbiol. 2019 Apr 1. pii: 10.1038/

   Inserts

   HBc from HBV AYW
   mNeonGreen
   

   Use

   Tags

   GGSGGSGGS linker

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-miRFP680-NES-p2A-ezrin-mRuby3-IRES-Neo

   Plasmid

   #222636

   Purpose

   Ezrin activation reporter

   Depositor

   Arnold Hayer

   Article

   Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

   Insert

   miRFP680-NES-p2A-ezrin-mRuby3 (EZR Human)
   

   Use

   Lentiviral

   Tags

   mRuby3

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. rAAV-syn::FLEX-rev::PSAML141F,Y115F:GlyR-IRES-GFP

   Plasmid

   #32481

   Purpose

   Cre-dependent expression of PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP marker

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

   Insert

   PSAML141F,Y115F-GlyR
   

   Use

   AAV and Cre/Lox; Adeno-associated virus, flex swi…

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   Synapsin

   Available since

   Jan. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1

   Plasmid

   #184397

   Purpose

   Donor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   Firefly luciferase beta-globin-PTC39
   

   Use

   CRISPR; Donor plasmid for hdr

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   PTC in beta-globin at amino acid position 39

   Promoter

   Tet-On 3G

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-Stop-NPM-ALK-MSNA-IRES-EGFP (MSNAIE)

    Plasmid

    #154470

    Purpose

    cDNA of NPM-ALK in the MSE backbone

    Depositor

    Cornelius Miething

    Article

    Shoumariyeh et al Oncogene. 2020 Feb;39(9):1904-1913. doi:

    Insert

    NPM-ALK
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

    Plasmid

    #52419

    Purpose

    Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    ERAS (ERAS Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-IRES-puro-NLS-NLS-dPba3Cas13b-3xGFP-NLS

    Plasmid

    #191369

    Purpose

    Overexpression of NLS-NLS-dPba3Cas13b-3xGFP-NLS in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-NLS-dPba3Cas13b-3xGFP-NLS
    

    Use

    Tags

    3xGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR_UCOE-EF1a-dCas9-XTEN80-KRAB(Kox1)-IRES-mCherry

    Plasmid

    #188769

    Purpose

    dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-IRES-puro-NLS-NLS-dHgm4Cas13b-3xGFP-NLS

    Plasmid

    #191368

    Purpose

    Overexpression of NLS-NLS-dHgm4Cas13b-3xGFP-NLS in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-NLS-dHgm4Cas13b-3xGFP-NLS
    

    Use

    Tags

    3xGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid

    #69546

    Purpose

    Tet-On system expressing MyoD-2A-GFP-2xNLS.

    Depositor

    Charles Gersbach

    Inserts

    eGFP
    Myod1 (Myod1 Mouse)
    

    Use

    Lentiviral

    Tags

    2x nuclear localization sequence and T2A "sk…

    Expression

    Mutation

    Removed stop codon to fuse it to the 2A-eGFPnls i…

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-IRES-puro-NLS-dAdmCas13d-EGFP-NLS-3xFlag

    Plasmid

    #132412

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dAdmCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    R269A; H274A; R837A; H842A

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMIR-Dsred-IRES-His-Halo-Tev-Keap C288S

    Plasmid

    #62459

    Purpose

    expresses his-tagged C288S human Keap1 protein

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    His-Halo-Tev

    Expression

    Mammalian

    Mutation

    Changed Cysteine 288 of Keap1 to serine

    Promoter

    CMV

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. NLS-GI-VP16-IRES-NLS-GAL4DBD-FKBP-SMBiT-FKF1

    Plasmid

    #183402

    Purpose

    Dual expression of GI-VP16 and GAL4DBD-FKBP-SmBiT-FKF1 in the nucleus

    Depositor

    Alice Ting

    Article

    Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

    Insert

    GI-VP16-IRES-GAL4DBD-FKBP-SMBiT-FKF1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-IRES-puro-NLS-dEsCas13d-EGFP-NLS-3xFlag

    Plasmid

    #132410

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dEsCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    R295A; H300A; R849A; H854A

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. AttB_[Kozak-mut]ACE2_IRES-mCherry-H2A-P2A-PuroR

    Plasmid

    #171595

    Purpose

    Weak human ACE2 abundance from Bxb1 landing pad

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Inserts

    ACE2 (ACE2 Human)
    mCherry
    H2A
    Pac
    

    Use

    Synthetic Biology; Promoterless bxb1 dna recombin…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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