Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 32,471 results for: Eng;

Showing: 5701 - 5750 of 32471 results

  10. pAAV-Ef1a-FAS-GCaMP6f-WPRE

    Plasmid
    #141237
    Purpose
    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)
    Depositor
    Guohong Cui
    Article
    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do
    Insert
    GCaMP6f
    Use
    AAV and Cre/Lox
    Tags
    6XHIS and Xpress
    Promoter
    Human elongation factor-1 alpha (EF-1 alpha)
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    141237-plasmid-map-sequence-id-431695

    Enlarge

  25. TMD18

    Plasmid
    #163673
    Purpose
    Expresses a ST6GAL1 mutant with 18 amino acids in its TMD in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    For this partial length ST chimera, two restricti…
    Available Since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163673-plasmid-map-sequence-id-322677

    Enlarge

  26. TMD19

    Plasmid
    #163674
    Purpose
    Expresses a ST6GAL1 mutant with 19 amino acids in its TMD in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    For this partial length ST chimera, two restricti…
    Available Since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163674-plasmid-map-sequence-id-322678

    Enlarge

  29. pgRNA_CV2

    Plasmid
    #167165
    Purpose
    pgRNA_CV2 is derived from gRNA_cloning vector (Addgene plasmid ID: 41824) by adding about 80 bps from the sgRNA sequence as well as an AgeI site. In the literature, sgRNA_AL is used as an alias.
    Depositor
    Alexander Loewer
    Article
    Sheng et al Cell Rep. 2019 Apr 2;27(1):48-58.e7. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available Since
    May 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167165-plasmid-map-sequence-id-330265

    Enlarge

  31. O-AARS-32

    Plasmid
    #166540
    Purpose
    scFv of a human scaffold targeting Alanyl-tRNA synthetase. Antigen coverage aa 1-968 of 968, full-length
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166540-plasmid-map-sequence-id-329233

    Enlarge

  33. pET-30a(+)-N-SH2

    Plasmid
    #111274
    Purpose
    express murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
    Tags
    HHHHHHENLYFQG
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111274-plasmid-map-sequence-id-215709

    Enlarge

  45. MG1655-tMMR

    Bacterial Strain
    #62392
    Purpose
    E. coli MG1655 temperature-sensitive mismatch repair deficient strain, harboring mutS(A134V) mutL(G62S), and nadA::Tn10 pglΔ8 [λ cI857 Δ(cro-bioA)]} for λ Red expression
    Depositor
    Csaba Pál
    Article
    Nyerges et al Nucleic Acids Res. 2014 Apr;42(8):e62. d
    Bacterial Resistance
    Tetracycline
    Species
    Escherichia coli
    Available Since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 5701 - 5750 of 32471 results