We narrowed to 23,428 results for: CRISPR

1. pCDNA3.1- dCas9-2xNLS-EGFP

   Plasmid

   #74710

   Purpose

   Expresses dCas9-2xNLS-EGFP in mammalian cells

   Depositor

   Eugene Yeo

   Article

   Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

   Insert

   dCas9-2xNLS-EGFP
   

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCE048-SiT-Cas12a

   Plasmid

   #128124

   Purpose

   Express both AsCas12a and CRISPR arrays on single transcripts

   Depositor

   Randall Platt

   Article

   Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

   Insert

   AsCas12a-Triplex
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC18-mini-Tn7T-Plac-dCas9 (Plac)

   Plasmid

   #105235

   Purpose

   Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosa

   Depositor

   Kristala Prather

   Article

   Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

   Insert

   S. pasteurianus dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Bacterial

   Mutation

   D10A, H599A

   Promoter

   Available Since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBx-Spas-sgRNA-Gm

   Plasmid

   #105232

   Purpose

   Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosa

   Depositor

   Kristala Prather

   Article

   Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

   Insert

   S. pasteurianus sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-EFS-PCP-GFPnls

   Plasmid

   #121938

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   PCP-GFPnls
   

   Use

   Lentiviral

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSET-dCas9

   Plasmid

   #110183

   Purpose

   Expression of the dCas9 gene in Streptomyces

   Depositor

   Yinhua Lu

   Article

   Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

   Insert

   dCas9
   

   Use

   CRISPR; I

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   ermE*p

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCpf1b

   Plasmid

   #122187

   Purpose

   Bacterial genome editing plasmid with kanamycin resistance marker and using sacB as a counter-selection marker

   Depositor

   Cheng-Cai Zhang

   Article

   Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPIPL12-FnCas12a

   Plasmid

   #199563

   Purpose

   Tetracycline-Inducible Fncas12a gene expression in E. coli and clostridia

   Depositor

   Philippe Lambin

   Article

   Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

   Insert

   Fncas12a
   

   Use

   CRISPR and Synthetic Biology; Tetracycline induci…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   COtetR-PIPL12

   Available Since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AcrIIA2

   Plasmid

   #101042

   Purpose

   Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

   Depositor

   Zhiwei Huang

   Article

   Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

   Insert

   AcrIIA2
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYL-kasOp-FnCas12a2

    Plasmid

    #177874

    Purpose

    Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

    Depositor

    Yunzi Luo

    Article

    Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

    Insert

    Streptomyces codon optimized FnCas12a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    kasOp*

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pdCas9-omega

    Plasmid

    #114632

    Purpose

    Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

    Depositor

    Anushree Chatterjee

    Article

    Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

    Insert

    dCas9-omega
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet promoter

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFnCpfAb-apr

    Plasmid

    #214198

    Purpose

    Expresses FnCpf1 and RecAb recombination system for genome editing in Acinetobacter baumannii

    Depositor

    Yu Wang

    Article

    Wang et al Curr Issues Mol Biol. 2024 Jan 5;46(1):5

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    tac promoter

    Available Since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W

    Plasmid

    #112927

    Purpose

    CRISPR-a reporter assay lentiviral vector (gTetO)

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    hU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPBO.ACT001

    Plasmid

    #182711

    Purpose

    aTc inducible dCasRx

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    dCasRx
    

    Use

    CRISPR

    Tags

    2x-FLAG

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pTet

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLH-stsgRNA3.1

    Plasmid

    #64118

    Purpose

    Vector for expression of St1 sgRNA3.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 sgRNA3.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSEVA421-Cas9tr

    Plasmid

    #138709

    Purpose

    Plasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenes

    Depositor

    Víctor de Lorenzo

    Article

    Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

    Insert

    tracrRNA-Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDHt/sk-CE

    Plasmid

    #92128

    Purpose

    Expression of codon-optimized Cas9-eGFP fusion protein with Pcbh1 promoter in Trichoderma reesei

    Depositor

    Zhihua Zhou

    Article

    Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

    Insert

    Cas9-eGFP
    

    Use

    CRISPR; Trichoderma reesei expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Pcbh2

    Available Since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. NAX_CAGGS_CAS9_m4_VPR_EBFP2

    Plasmid

    #167881

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113343

    Purpose

    Expresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    p300core-dCas9-VP64-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCpf1-sp

    Plasmid

    #122186

    Purpose

    Bacterial genome editing plasmid with spectinomycin resistance marker

    Depositor

    Cheng-Cai Zhang

    Article

    Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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