We narrowed to 15,989 results for: LEA

1. MSNA-c028

   Plasmid

   #175470

   Purpose

   Protein expression in bacterial cells. FERM domain, M1-E346. Contains H288A point mutation that reduces binding to CD44.

   Depositor

   Opher Gileadi

   Article

   Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

   Insert

   MSN (MSN Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   H288A

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSNA-c021

   Plasmid

   #175468

   Purpose

   Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R point mutation that reduces binding to CD44.

   Depositor

   Opher Gileadi

   Article

   Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

   Insert

   MSN (MSN Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   L281R

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSNA-c001

   Plasmid

   #175466

   Purpose

   Protein expression in bacterial cells. FERM domain, M1-E346. Used for crystallography (PDB:6TXQ).

   Depositor

   Opher Gileadi

   Article

   Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

   Insert

   MSN (MSN Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xEGFP-mMeikin(1-391) - hRad21(142-275, E169R, R172E) - mMeikin(387-434)

   Plasmid

   #174713

   Purpose

   Mammalian expression of mouse Meikin with inserted C-terminal Separase cleavage site inactivated

   Depositor

   Iain Cheeseman

   Article

   Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

   Insert

   Meikin (Meikin Mouse)
   

   Tags

   3xEGFP

   Expression

   Mammalian

   Mutation

   mMeikin: 1-391; hRad21: 142-275, E169R, R172E; mM…

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3HA-AMOT p130∆PPxY1(E104K/E105K)

   Plasmid

   #171130

   Purpose

   expresses AMOT(E104K/E105K) in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   Angiomotin p130 E104K/E105K (AMOT Human)
   

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   changed glutamic acid residues 104 and 105 to lys…

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. KLHL31 pgk (1088)

   Plasmid

   #62031

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   KLHL31 (Klhl31 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pCDH-EF1-E-'No'Mi-Red-T2A-Puro

   Plasmid

   #246642

   Purpose

   EF-1alpha driven EV reporter E-'No'Mi-R, a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins. Nanoluc and Flag tag are removable with TEVp.

   Depositor

   Koen Breyne

   Article

   Obuchi et al J Extracell Vesicles. 2025 Jun;14(6):e70

   Insert

   cleavable E-NoMi-Red
   

   Use

   Lentiviral

   Tags

   FLAG-Nanoluciferase and mCherry

   Expression

   Mammalian

   Promoter

   EF-1alpha

   Available Since

   Oct. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-NLS-actin-R62D

   Plasmid

   #118381

   Purpose

   Gateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Mutation

   NLS and R62D

   Available Since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. SuExp His-Myc-hPLD3

   Plasmid

   #173851

   Purpose

   Protein expression plasmid for recombinant His-Myc tag human PLD3

   Depositor

   David Nemazee

   Article

   Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

   Insert

   PLD3 (PLD3 Human)
   

   Tags

   6xHis, Myc, Factor X cleavable

   Expression

   Mammalian

   Mutation

   intracellular and transmembrane domain truncated,…

   Promoter

   CMV

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. SuExp His-Myc-hPLD4

    Plasmid

    #173852

    Purpose

    Protein expression plasmid for recombinant His-Myc tag human PLD4

    Depositor

    David Nemazee

    Article

    Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

    Insert

    PLD4 (PLD4 Human)
    

    Tags

    6xHis, Myc, Factor X cleavable

    Expression

    Mammalian

    Mutation

    intracellular and transmembrane domain truncated,…

    Promoter

    CMV

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET24TEV-tSHIP1

    Plasmid

    #183770

    Purpose

    Expresses C-terminal truncated SHIP1 (tSHIP1)with C-terminal TEV and 6HIS tags from pET24 backbone

    Depositor

    William Kerr

    Article

    Sudan et al Front Immunol. 2022 May 4;13:830961. doi

    Insert

    SHIP1 (INPP5D Human)
    

    Tags

    6HIS and Tobacco Etch Virus (TEV) Cleavage site

    Expression

    Bacterial

    Mutation

    includes aa1 to aa883 only

    Promoter

    T7 lac

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-InPGrp-Kras

    Plasmid

    #236001

    Purpose

    Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to plasma membrane.

    Depositor

    Jin Zhang

    Article

    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

    Insert

    InPGrp-Kras (Cyth3 Rat, Synthetic)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEcNucC

    Plasmid

    #214050

    Purpose

    Bacterial expression plasmid for E. coli MS 115-1 NucC, a cA3-responsive nuclease known to cause abortive infection; used as a positive control in the Haliangium ochraceum type III CRISPR-Cas system

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    EcNucC
    

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lacUV5 promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pStreptagIIx2 Alfatag 3C InsR EABR

    Plasmid

    #234996

    Purpose

    For production of Extracellular Vesicles (EVs), with the Insulin Receptor Strep-tag II labeled on their surface

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    InsR-EABR (INSR Human)
    

    Tags

    2xStreptag-II, Alfatag, HRV 3C site

    Expression

    Mammalian

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNIC-ZB-SPRTNwt-FL

    Plasmid

    #110217

    Purpose

    Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.

    Depositor

    Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Expression

    Bacterial

    Mutation

    P296L (see depositor comments below)

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pαH-S-GSAS/D614G

    Plasmid

    #164566

    Purpose

    SARS-CoV-2 Spike protein with D614G mutation (S-GSAS-D614G variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-GSAS-D614G variant)
    

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. A3Ai-Cas9n-UGI-NLS

    Plasmid

    #109425

    Purpose

    Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Insertion of an L1 intron into the coding sequenc…

    Promoter

    CMV

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. WT USP7 TRAF

    Plasmid

    #227226

    Purpose

    Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Article

    Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    aa 62-205

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMT-sox10-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80063

    Purpose

    Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Promoter

    Sox10 promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. TAPBPL pmRFP-N2 (721)

    Plasmid

    #62000

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    TAPBPL (TAPBPL Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only