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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,582 results for: eng

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Showing: 2281 - 2300 of 14582 results

  3. pcDNA3.1-FGFR1c-V5/HIS

    Plasmid
    #201106
    Purpose
    expression of human FGFR1 receptor tyrosine kinase in mammalian cells
    Depositor
    Pavel Krejčí
    Publication
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human FGFR1 receptor tyrosine kinase, variant c, full length, wildtype (FGFR1 Human)
    Tags
    V5/His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    201106-plasmid-map-sequence-id-394496

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  5. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid
    #91249
    Purpose
    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR
    Depositor
    Ian Wheeldon
    Publication
    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.
    Inserts
    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91249-plasmid-map-sequence-id-181276

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  10. pCMV-ABE8.8m-VRQR-P2A-EGFP (BKS971)

    Plasmid
    #242653
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with nVRQR(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-ABE8.8m-SpCas9-VRQR-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.8 mutations in TadA, VRQR mutations in SpCas…
    Promoter
    CMV and T7
    Available since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242653-plasmid-map-sequence-id-488991

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  12. MSP977

    Plasmid
    #65774
    Purpose
    Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E mutation in Cas9
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65774-plasmid-map-sequence-id-112656

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  15. pCAG Cas9-2A-Citrine

    Plasmid
    #92358
    Purpose
    CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9 2A Citrine
    Expression
    Mammalian
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92358-plasmid-map-sequence-id-178229

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  19. tTA_BFP

    Plasmid
    #58854
    Purpose
    Constitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901)
    Depositor
    Joshua Leonard
    Publication
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tTA transcription factor fused to enhanced blue fluorescent protein
    Tags
    BFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58854-plasmid-map-sequence-id-327655

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Showing: 2281 - 2300 of 14582 results