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pLenti-NG-PE2-BSD

Plasmid

#176933

Purpose

Expresses PE2-NG variant in mammalian cells

Depositor

Hyongbum Kim

Publication

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Insert

PE2-NG variant

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EFS

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDL52 (clone45)

Plasmid

#188578

Purpose

High-yield production of coenzyme F420 in E. coli

Depositor

Gerald Lackner

Publication

Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

Inserts

ribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE

Expression

Bacterial

Mutation

Codon optimization, synthetic ribosome binding si…

Promoter

T7 variants

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCas9-beta

Plasmid

#169240

Purpose

Cas9 and beta expression plasmid for performing gene knockouts via oligo recombineering

Depositor

Brian Pfleger

Publication

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Inserts

cas9
beta

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pBbE1k-2

Plasmid

#59945

Purpose

dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

Depositor

Taek Soon Lee

Publication

Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

Inserts

dihydropterine reductase
pterin-4a-carbinolamine dehydratase

Promoter

pTrc

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pEM8733

Plasmid

#213992

Purpose

PrpsM driven expression of mCherry

Depositor

Marc Erhardt

Publication

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mCherry

Expression

Bacterial

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCS-APTA

Plasmid

#247077

Purpose

pCS27 containing aroL, ppsA, tktA, aroG(fbr) from E. coli

Depositor

Yajun Yan

Publication

Shen et al Metab Eng. 2017 Jul;42:52-58. doi: 10.10

Inserts

aroL
ppsA
tktA
aroG(fbr)

Expression

Bacterial

Mutation

feedback-inhibition-resistant

Promoter

PLlacO1

Available since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-EV

Plasmid

#202466

Purpose

Empty vector of pSMART-HCKan

Depositor

Michael Lynch

Publication

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EM7-Erv1p

Plasmid

#202484

Purpose

Expresses sulfhydryl oxidase (Erv1p) constitutively

Depositor

Michael Lynch

Publication

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)

Expression

Bacterial

Promoter

EM7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pHB-2

Plasmid

#140955

Purpose

For PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.

Depositor

Kang Zhou

Publication

Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

Insert

phaCAB

Expression

Bacterial

Mutation

R238S mutation in PhaC

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pETM6-Pc4CL-PhCHS-MsCHI

Plasmid

#73396

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Publication

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Pc4CL
PhCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTS2374-Tier1-PhCMV-Gal4_VP16

Plasmid

#169582

Purpose

Tier-1 vector encoding PhCMV-driven Gal4-VP16 transactivator (PhCMV-Gal4-VP16-pA).

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

GAL4-transactivator

Expression

Mammalian

Promoter

PhCMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pVH234-Tier1-PmPGK1-tTA

Plasmid

#169574

Purpose

Tier-1 vector encoding PmPGK1-driven tTA expression (PmPGK1-tTA-pA).

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled transactivator

Expression

Mammalian

Promoter

PPGK

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pVH41-Tier1-PhCMV-GLuc

Plasmid

#169544

Purpose

Tier-1 vector encoding PhCMV-driven gaussia luciferase (PhCMV-GLuc-pA)

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven Gaussia Luciferase

Expression

Mammalian

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-Hsp-eGFP-PGK-mCherry

Plasmid

#191862

Purpose

Heat inducible eGFP reporter with constitutive mCherry marker

Depositor

Yingxiao Wang

Publication

Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

Inserts

eGFP
mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

HSPA7 promoter and PGK promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBS1ClacZ

Plasmid

#55171

Purpose

lacZ-reporter vector, integration at amyE, ampr, cmr

Depositor

Thorsten Mascher

Publication

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Promoter

none

Available since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only
pEM8733

Plasmid

#213992

Purpose

PrpsM driven expression of mCherry

Depositor

Marc Erhardt

Publication

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mCherry

Expression

Bacterial

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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LYS-Leu2

Plasmid

#218114

Purpose

Integration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.

Depositor

Carl Denard

Publication

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GFP-TAG

Plasmid

#158131

Purpose

Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Publication

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

sfGFP

Expression

Yeast

Mutation

Introduced a stop codon in place of the tyrosine …

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat6

Plasmid

#45134

Depositor

Christopher Petzold

Publication

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT6

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat18

Plasmid

#45146

Depositor

Christopher Petzold

Publication

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT18

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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