We narrowed to 29,245 results for: pET

1. pET-20b-M-Ntx-S1

   Plasmid

   #58716

   Purpose

   Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S

   Depositor

   Fiorella Tonello

   Article

   Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

   Insert

   Notexin
   

   Tags

   Methionine

   Expression

   Bacterial

   Mutation

   Changed Asparagine 1 to Serine

   Promoter

   T7

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28b-GBP-MTn

   Plasmid

   #154363

   Purpose

   Bacterial expression of MTn

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Insert

   MTn
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-PKM2-H391Y

   Plasmid

   #122692

   Purpose

   Express His-tagged PKM2 (H391Y) protein in bacteria.

   Depositor

   Wen-Ching Wang

   Article

   Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

   Insert

   Pyruvate kinase M2 isoform (PKM Human)
   

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   H391Y

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a HAT epitope

   Plasmid

   #129445

   Purpose

   expression of HAT epitope

   Depositor

   Oskar Laur

   Insert

   HAT epitope
   

   Expression

   Bacterial

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETSUMO2_Bacteroidetes-bGSDM-TEV

   Plasmid

   #222101

   Purpose

   For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

   Insert

   Bacteroidetes-bGSDM-TEV ORF
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   TEV protease site replaces residues E242–G248

   Promoter

   T7

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETZZtevPP7CP(C68A,C73A)

   Plasmid

   #27548

   Depositor

   Kathleen Collins

   Article

   Hogg et al RNA. 2007 Jun . 13(6):868-80.

   Insert

   Pseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)
   

   Tags

   TEV protease cleavage site, six histidine tag, an…

   Expression

   Bacterial

   Mutation

   changed two cysteine to alanine (C68A,C73A) to in…

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-His6-FnCas9GFP

   Plasmid

   #130966

   Purpose

   expression of FnCas9-GFP in bacterial cells

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

   Insert

   HA-NLS-FnCas9
   

   Use

   CRISPR

   Tags

   6xHis-mEGFP-TEV-HA-NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET 2C-T10-dCasX

   Plasmid

   #126418

   Purpose

   E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Insert

   DpbCasX
   

   Use

   CRISPR

   Tags

   His-MBP

   Expression

   Bacterial

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28_6xHis-PARP1-ART

   Plasmid

   #174810

   Purpose

   Bacterial expression of isolated PARP1 ART subdomain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-ART (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28_6xHis-PARP1-BRCT

    Plasmid

    #174807

    Purpose

    Bacterial expression of isolated PARP1 BRCT domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-BRCT (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28_6xHis-PARP1-WGR

    Plasmid

    #174808

    Purpose

    Bacterial expression of isolated PARP1 WGR domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-WGR (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-6xHis-mCherry(-6)

    Plasmid

    #205045

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    mCherry(-6)
    

    Tags

    MGHHHHHGG

    Expression

    Bacterial

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pet16b-Avi-ubiquitin_rbs_BirA

    Plasmid

    #134897

    Purpose

    Expresses HIS-Avi-tagged ubiquitin for recombinant protein production

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Inserts

    Ubiquitin (UBB Human)
    BirA
    

    Tags

    10HIS-AviTag-PreScission(3C)protease

    Expression

    Bacterial

    Promoter

    RBS and T7

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21b-Caspase-3(V266E)

    Plasmid

    #90089

    Purpose

    Expresses caspase 3 V266E mutant in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

    Insert

    Caspase 3 (CASP3 Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    V266E

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET30a-His6-LOXCATmut

    Plasmid

    #140085

    Purpose

    A control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Mutation in H265A and R268A in LOX (used original…

    Promoter

    T7

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET24b-SUMO-BG2.4

    Plasmid

    #199438

    Purpose

    expresses BAmGreen2.4 in bacterial cells with a SUMO tag

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMGreen2.4
    

    Tags

    his tag and sumo

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET_MBP-RGG-RGG

    Plasmid

    #124946

    Purpose

    Expression of MBP-RGG-RGG

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    MBP-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hPU.1ΔN165-pET28b

    Plasmid

    #199796

    Purpose

    Expressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coli

    Depositor

    Gregory Poon

    Article

    Xhani et al Sci Adv. 2020 Feb 21;6(8):eaay3178. doi:

    Insert

    spi1 (SPI1 Human)
    

    Tags

    None

    Expression

    Bacterial

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-eGFP-MinD

    Plasmid

    #133622

    Purpose

    Expression vector for the Escherichia coli MinD with an N-terminal fusion of a His-tag for purification and an EGFP for visualization.

    Depositor

    Petra Schwille

    Article

    Zieske et al FEBS Lett. 2014 Aug 1;588(15):2545-9. do

    Insert

    EGFP-MinD (minD E. coli)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET23b-Casp6-His

    Plasmid

    #11823

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 6 (CASP6 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET-SUMO-mGSDMD

    Plasmid

    #111560

    Purpose

    Express mouse GSDMD in E.coli

    Depositor

    Hongbo Luo

    Article

    Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Tags

    His, SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28_mouse-pro-IL1B

    Plasmid

    #183386

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    pro-IL1B (Il1b Mouse)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET21b-RBD-JA1

    Plasmid

    #167126

    Purpose

    Expresses SARS-CoV-2 RBD in bacterial system

    Depositor

    David Margulies

    Article

    Ahmad et al bioRxiv. 2021 Jan 27. doi: 10.1101/2021.

    Insert

    SARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    residues (333 to 529 aa)

    Promoter

    T7

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET21-pelB-GST-4

    Plasmid

    #228986

    Purpose

    For bacterial expression of anti-GST nanobody GST-4, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    GST-4
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET21-pelB-LaRIgG-2

    Plasmid

    #228996

    Purpose

    For bacterial expression of anti-rabbit IgG nanobody LaRIgG-2, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaRIgG-2
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET21-pelB-LaGIgG-12

    Plasmid

    #228999

    Purpose

    For bacterial expression of anti-mouse IgG nanobody LaMIgG-12, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaGIgG-12
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET21-pelB-GST-3

    Plasmid

    #228985

    Purpose

    For bacterial expression of anti-GST nanobody GST-3, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    GST-3
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET14b – lambda exonuclease

    Plasmid

    #104531

    Purpose

    bacterial expression of lambda exonuclease

    Depositor

    Charles Bell

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2011 Jul 19;10

    Insert

    λ exonuclease
    

    Tags

    His6 followed by a thrombin site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-APOBEC1-YTH

    Plasmid

    #200158

    Purpose

    For bacterial expression of the APOBEC1-YTH fusion protein for protein purification

    Depositor

    Kate Meyer

    Article

    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

    Insert

    APOBEC1-YTH
    

    Tags

    6xHis, HA, and Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    Codon optimized for E coli expression

    Promoter

    LacZ

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 6his-WT gusA-pET28a+

    Plasmid

    #61156

    Purpose

    N -terminal His tagged wild-type GUS expression vector

    Depositor

    Andrew Ellington, Ichiro Matsumura

    Article

    Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

    Insert

    gusA
    

    Tags

    6x His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge