We narrowed to 44,063 results for: tro
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Plasmid#67876Purposeencodes shy1 with a C-terminal 3xHA-tag under control of its endogenous promoter.DepositorInsertshy1
UseExpression in ustilago maydisTagsHAPromotershy1Available SinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFGL1169R
Plasmid#116899PurposeCenpC:mCherry (centromere marker)DepositorInsertsCenp C (partial)
mCherry
CenpC downstream region
UseFungal expression (in magnaporthe oryzae)Mutationtruncated, no stop codon and without start codonAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRCHisB-TRF2∆B
Plasmid#53208PurposeExpressed N-terminally Hexahistine tagged TRF2 Delta Basic proteinDepositorInsertTRF2 Delta Basic (TERF2 Human)
Tags6x HisExpressionBacterialMutationcontains amino acids 47-500PromoterTrcAvailable SinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
141_pT3TS_iCre
Plasmid#171793PurposePlasmid for optimised iCre RNA synthesis using T3 promoter.DepositorInsertoptimised Cre for RNA expression
ExpressionBacterialPromoterT3Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-001
Plasmid#78688PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMP-SMYD2_1
Plasmid#36349DepositorAvailable SinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Glt1a
Plasmid#98705Purposemammalian expression of Glt1aDepositorInsertGlt1a (Slc1a2 Rat)
ExpressionMammalianMutationI288V (please see depositor comments below)PromoterCMVAvailable SinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1K3_pBen-sfGFP_J23101-BenR
Plasmid#128125PurposeExpressing sfGFP under control of benzoate responsive promoter (pBen) and expressing BenR transcription factor gene under constitutive promoter J23101, and RBS B0032DepositorInsertsfGFP and BenR
UseSynthetic BiologyExpressionBacterialPromoterpBen and J23101Available SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK300
Plasmid#87766Purposeempty control plasmid, three terminators to place inserts between for insulation, ampR, pBR322 origin, lacIDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterNoneAvailable SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-TAPP1-PH
Plasmid#67675PurposeMultisite gateway entry clone for adding C-terminal fusions of TAPP1-PHDepositorInsertTAPP1-PH
UseGateway multisite 3' entry cloneAvailable SinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 nt 400-1782 delta440-500 delta1449-1735
Plasmid#60633PurposeZKSCAN1 circular RNA expression plasmid containing minimal sufficient intronsDepositorAvailable SinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFGL1079
Plasmid#116897PurposeCenpC:eGFP (centromere marker)DepositorInsertsCenp C (partial)
eGFP
CenpC downstream region
UseFungal expression (in magnaporthe oryzae)Mutationtruncated, no stop codon and without start codonAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLS.546
Plasmid#184963PurposeTest effect of a1/a2 length on editing priB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 22
ExpressionBacterialMutationpriB donor G1069A, a1/a2 length extended to 22 bpPromoterT7/lacAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAH35 (Pmyo-2::cGAL-N::let-858 3'UTR)
Plasmid#107134PurposecGAL-N driver under the control of C. elegans myo-2 promoterDepositorInsertNLS::cGAL(DBD)::gp41-1-N-intein
ExpressionWormPromoterC. elegans myo-2 promoterAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+) GFP-PIPKI{gamma}661-FLAG
Plasmid#29583DepositorInsertphosphatidylinositol phosphate kinase type I gamma
TagsFlag and GFPExpressionMammalianMutationN/AAvailable SinceFeb. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET5
Plasmid#107437PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET5 (MET5 Budding Yeast)
TagsV5-HisExpressionYeastMutationV81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …PromoterGAL1Available SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_L4(EAAAK)3
Plasmid#131211PurposeCloning vector containing A-B-C casette with linker L4 in site BDepositorInsert(EAAAK)3 linker
ExpressionMammalianPromoterCMVAvailable SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAGM55361
Plasmid#153227PurposeControl plasmid for knockout of AP3 geneDepositorInsertCas9, guide RNA for AP3 and FAST marker
UseSynthetic BiologyAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLS.540
Plasmid#184959PurposeTest effect of a1/a2 length on editing fabH using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 22
ExpressionBacterialMutationfabH donor G954A, a1/a2 length extended to 22 bpPromoterT7/lacAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hMSH2_G674A
Plasmid#134874PurposeExpression human MSH2 G674 mutant protein using baculovirusDepositorInserthuman MSH2_G674A (MSH2 Human)
ExpressionInsectMutationChanged Glycine to Alanine at aa#674Available SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
CDS_GUS
Plasmid#136084PurposeFor histochemical _-glucuronidase activity assays. This GUS CDS does not have an intron.DepositorInsertCDS_GUS
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMB1-spect-PthrC3-eGFP
Plasmid#107409PurposeThis plasmid contain promoter PthrC3, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.DepositorInserteGFP
ExpressionBacterialPromoterPthrC3Available SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pBALU9
Plasmid#69324PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd GFP intron;use C-term after STOPDepositorInsertgSL2-NLS-GFPint-FgF
ExpressionBacterialAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0040
Plasmid#117607PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)DepositorInsert[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSMP-SMYD2_2
Plasmid#36350DepositorAvailable SinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0038
Plasmid#117605PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for LbCas12a(pEPOR1CB0013) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0029)DepositorInsert[35S:LbCas12a(pEPOR1CB0013) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM53
Plasmid#154156PurposeFirefly luciferase under the control of 8 copies of the Aedes aegypti Rel1A NF-κB siteDepositorInsertFirefly luciferase
UseLuciferasePromoter8 copies of the Aedes Aegypti Rel1A NF-κB promoterAvailable SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-Top-T~Bottom-T
Plasmid#87315PurposeControl plasmid. Encodes the Split-Broccoli system (Top + Bottom) with only "Top" under the control of E. coli sigma 70 promoter.DepositorInsertSplit-Broccoli system (promoter control)
UseSynthetic BiologyExpressionBacterialPromoterP70aAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBALU12
Plasmid#69328PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd mChO intron;use C-term after STOPDepositorInsertgSL2-NLS-mChOint-FgF
ExpressionBacterialAvailable SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN1232
Plasmid#127164PurposeVector for integrating the controller (constitutive expression of GEM-degronSwitch and pZ3 expression of key)DepositorInsertpRPL18B-GEM-degronSwitchA-tENO2-pZ3-keyA-mTurquoise2-NLS(SV40)-tTDH1
UseSynthetic BiologyAvailable SinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-BTK-PH
Plasmid#67673PurposeGateway entry clone containing BTK-PH with no stop codonDepositorInsertBTK-PH-NS
UseGateway multisite middle entry cloneAvailable SinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_L3(GSSSSGGSSSG)
Plasmid#131210PurposeCloning vector containing A-B-C casette with linker L3 in site BDepositorInsertGSSSSGGSSSG llinker
ExpressionMammalianPromoterCMVAvailable SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_L2(GSSGGSSG)
Plasmid#131209PurposeCloning vector containing A-B-C casette with linker L2 in site BDepositorInsertGSSGGSSG linker
ExpressionMammalianPromoterCMVAvailable SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_L1(GSSSG)
Plasmid#131208PurposeCloning vector containing A-B-C casette with linker L1 in site BDepositorInsertGSSSG linker
ExpressionMammalianPromoterCMVAvailable SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0039
Plasmid#117606PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for AsCas12a(pEPOR1CB0014) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0028)DepositorInsert[35S:AsCas12a(pEPOR1CB0014) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_MxCarH-CBD
Plasmid#131189PurposeExpresses MxCarH-CBD (Myxococcus xanthus) preceded by restriction sitesDepositorInsertMxCarH-CBD
ExpressionMammalianPromoterCMVAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_TtCarH-CBD
Plasmid#131190PurposeExpresses TtCarH-CBD (Thermus termophilus) preceded by restriction sitesDepositorInsertTtCarH-CBD
ExpressionMammalianPromoterCMVAvailable SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-N-ZAP-Zeo
Plasmid#17382DepositorInsertNZAP (Zc3hav1 Rat)
UseRetroviralExpressionMammalianMutationN-terminal region of ZAP (amino acids 1-254)Available SinceMarch 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSB4C5_J23101-CocE
Plasmid#128129PurposeExpressing CocE enzyme (cocaine to benzoate) gene under control of the constitutive promoter J23101, and RBS B0032DepositorInsertCocE
UseSynthetic BiologyExpressionBacterialPromoterJ23101Available SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only