We narrowed to 23,428 results for: CRISPR

1. pRU53

   Plasmid

   #167679

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEM-3XP3.RFP-tan

   Plasmid

   #83474

   Purpose

   RFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

   Depositor

   Patricia Wittkopp

   Article

   Lamb et al Fly (Austin). 2016 Aug 5:1-12.

   Insert

   3XP3.RFP-tan
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   3XP3 eye-expression Promoter

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. NAX_CAGGS_CAS9_m4_Blasto

   Plasmid

   #167864

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP

   Plasmid

   #86987

   Purpose

   For cloning and expression of sgRNA together with expression of Cas9 and TagRFP

   Depositor

   Ralf Kuehn

   Article

   Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. NAX_CAGGS_CAS9_m4_VPR_mcherry

   Plasmid

   #167886

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

6. pRU206

   Plasmid

   #167693

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   PcUBQ4-2::asCpf1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRU322

   Plasmid

   #167683

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::asCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRU54

   Plasmid

   #167680

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRE57-Ter

   Plasmid

   #170635

   Purpose

   DNA donor for transposition

   Depositor

   Sheng Yang

   Article

   Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

   Insert

   Mini-Tn6677, Ter cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. MLM3639

    Plasmid

    #42252

    Purpose

    Cas9 expression vector

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    Streptococcus pyogenes Cas9-3X Flag
    

    Use

    CRISPR; Zebrafish expression

    Tags

    3X Flag

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTX171

    Plasmid

    #89258

    Purpose

    Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSMVP-Cas9N

    Plasmid

    #80934

    Purpose

    Plasmid that expresses Cas9N in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNW3

    Plasmid

    #53372

    Purpose

    Csy4 and Cas9 D10A nickase expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Csy4-T2A-Cas9n (D10A)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRU293

    Plasmid

    #167688

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSMVP-Cas9C

    Plasmid

    #80939

    Purpose

    Plasmid that expresses Cas9C in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPBO.ACT003

    Plasmid

    #182713

    Purpose

    aTc inducible dCasRx-IF3

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    dCasRx
    

    Use

    CRISPR

    Tags

    3x(GGGS)-Escherichia Coli Initiation Factor 3

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pTet

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pV132-vCas9

    Plasmid

    #203223

    Purpose

    Mammalian expression of vCas9

    Depositor

    Vikash Chauhan, Phil Sharp, Robert Langer

    Article

    Chauhan et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

    Insert

    vCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    S55R R976A K1003A T1314R

    Promoter

    CBh

    Available Since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry

    Plasmid

    #170182

    Purpose

    Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Insert

    mCherry
    

    Use

    Tags

    3XFlag and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCCM935

    Plasmid

    #58202

    Purpose

    Expresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR experiments.

    Depositor

    Craig Mello

    Article

    Kim et al Genetics. 2014 May 30. pii: genetics.114

    Insert

    unc-22 sgRNA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available Since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pADH119

    Plasmid

    #90985

    Purpose

    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb LEUpOUT CRISPR system.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    gRNA conserved, C. albicans LEU2 2 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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