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Punc-4c.TIR1

Plasmid

#194053

Purpose

TIR1 expression vector under the unc-4c promoter expressed in the DA motor neurons in C. elegans

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

TIR1 (TIR1 Mustard Weed)

Expression

Worm

Mutation

codon-optimized for expression in C. elegans; Two…

Promoter

unc-4c

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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CFP-GAI(1-92)

Plasmid

#37307

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Tags

CFP

Expression

Mammalian

Mutation

contains aa 1-92

Promoter

CMV

Available Since

June 19, 2012

Availability

Academic Institutions and Nonprofits only
pGal4BD-CRY2PHR

Plasmid

#28244

Purpose

Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

cry2 (CRY2 Mustard Weed)

Tags

Gal4BD

Expression

Yeast

Available Since

March 23, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CibN-dCas9-CibN

Plasmid

#60553

Purpose

Expresses CibN-dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-P4:gs:nls:CIBN

Plasmid

#135986

Purpose

Expresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

P4 coiled-coil fused to the CIBN domain

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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HBT-pcoCas9

Plasmid

#52254

Purpose

transient expression of pcoCas9 gene in plant cells

Depositor

Jen Sheen

Article

Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

Insert

pcoCas9

Use

CRISPR; Plant expression

Tags

FLAG and NLS

Promoter

hybrid constitutive promoter 35SPPDK

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pNat-AID*-9myc

Plasmid

#99523

Purpose

C-terminal AID*-9myc degron cassette with NatR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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DR5rev::erRFP

Plasmid

#61011

Purpose

Auxin signalling reporter (DR5rev) driving RFP (ER-targeted)

Depositor

Alexis Maizel

Article

Marin et al Plant Cell. 2010 Apr;22(4):1104-17. doi:

Insert

DR5rev::erRFP:35S_term

Expression

Plant

Promoter

DR5rev::erRFP:35Sterm

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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5aa SunTag VP64 EVD

Plasmid

#115483

Purpose

CRISPR-Cas9 SunTag system to target VP64 to the EVD/ATR loci each with two guide RNAs

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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mito-CIBN-GFP

Plasmid

#89480

Purpose

Encodes for a fusion protein between a mitochondrial anchor and the CIBN-GFP protein.

Depositor

Xavier Trepat Guixer

Article

Valon et al Nat Commun. 2017 Feb 10;8:14396. doi: 10

Insert

mito-CIBN-GFP (CIB1 Human, Mustard Weed)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-BICDN-CIBN

Plasmid

#102254

Purpose

Expresses fusion of dynein adapter BICDN (1-594) with GFP and CIB1 (1-170)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

GFP-BICDN-CIB1 (CIB1 Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE651

Plasmid

#153229

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCIB1(deltaNLS)-pmGFP

Plasmid

#28240

Purpose

Expresses CIB1 fused to plasma membrane-localized EGFP

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CIB1(deltaNLS)-pmGFP (CIB1 Mustard Weed)

Tags

EGFP

Expression

Mammalian

Mutation

NLS sequences mutated

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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linker-mCitrine-PIF6-NAT

Plasmid

#51576

Purpose

Tag protein of interest with PIF6 in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PIF6 (PIL2 Mustard Weed)

Tags

linker and mCitrine

Expression

Yeast

Mutation

1-100aa of PIF6

Promoter

none

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pHis-AID*-9myc

Plasmid

#99524

Purpose

C-terminal AID*-9myc degron cassette with HIS3MX marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)

Plasmid

#73404

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
PhCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAL175

Plasmid

#22276

Purpose

Expresses YFP-PIF for red-light-induced binding to Phy

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Tags

mYFP

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pSMALB

Plasmid

#161786

Purpose

Gateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and TagBFP.

Depositor

John Dick, Peter van Galen

Article

van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Promoter

Bidirectional minhCMV and SFFV

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-PEX3

Plasmid

#51572

Purpose

Constitutively express peroxisome localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

PEX3 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pHyg-AID*-9myc

Plasmid

#99518

Purpose

C-terminal AID*-9myc degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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