We narrowed to 32,675 results for: eng

1. pLVTHM-mSox17-3xflag

   Plasmid

   #206377

   Purpose

   Expresses 3xflag fused mouse SOX17 in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

   Insert

   Sox17 (Sox17 Mouse)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ290

   Plasmid

   #129670

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AacCas12b
   

   Use

   CRISPR; Gateway compatible aaccas12b entry clone

   Expression

   Plant

   Mutation

   AacCas12b is rice codon optimized

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDH42(CUG/A)

   Plasmid

   #154132

   Purpose

   Expresses engineered (23S-5S phusion) rRNAs operon in bacterial cells

   Depositor

   Alexander Mankin

   Article

   Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

   Insert

   engineered bacterial rRNAs operon under control of pL promoter
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK-Myc-Smurf1 delHECT

   Plasmid

   #13677

   Depositor

   Ying Zhang

   Article

   Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

   Insert

   Smurf1 deltaHect (SMURF1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Smurf1 without HECT domain (deleted amino acids 3…

   Available Since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pYPQ292

   Plasmid

   #129672

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b
   

   Use

   CRISPR; Gateway compatible aacas12b entry clone

   Expression

   Plant

   Mutation

   AaCas12b is rice codon optimized

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ141-ZmUbi-RZ-Cas12j2

   Plasmid

   #173926

   Purpose

   Gateway entry clone (attL5 & attL2) for Cas12j2 crRNA expression under ZmUbi promoter with ribozyme processing

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKMV-VioC

   Plasmid

   #65349

   Purpose

   systhesis of vioC gene in the violacein pathway

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   vioC
   

   Use

   Synthetic Biology

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEL638

   Plasmid

   #189831

   Purpose

   The T-DNA backbone of nCas12j2 for genome editing in rice

   Depositor

   Yong Zhang

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Type

   Empty backbone

   Use

   CRISPR; T-dna clone

   Promoter

   ZmUbi1

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMBA323

   Plasmid

   #214751

   Purpose

   To create delta(glmS,nadE) working strains and maintaining their viability

   Depositor

   Tae Seok Moon

   Article

   Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   BBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ-Cas12j2

    Plasmid

    #173924

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Insert

    NLS-Cas12j2-NLS
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Plant

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBTK102

    Plasmid

    #110574

    Purpose

    BTK Type 2 promoter for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    T7 promoter with RBS
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pKMV-VioE

    Plasmid

    #65351

    Purpose

    systhesis of vioE gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioE
    

    Use

    Synthetic Biology

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKMV-VioA

    Plasmid

    #65347

    Purpose

    systhesis of vioA gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioA
    

    Use

    Synthetic Biology

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBTK001

    Plasmid

    #110573

    Purpose

    Broad-host-range toolkit (BTK) entry vector, with superfolder GFP

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCDNA3.1-S-Luc_mito

    Plasmid

    #113927

    Purpose

    Plasmid encoding the mitochondrial localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    S-Luc_mito
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTX198

    Plasmid

    #89262

    Purpose

    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Insert

    OsPDS-gRNA01
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Promoter

    Maize Ubiquitin1 promoter

    Available Since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pR540

    Plasmid

    #207885

    Purpose

    template for 2 gRNA amplification

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    scaffold-pU6
    

    Expression

    Bacterial

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS021

    Plasmid

    #207917

    Purpose

    Level 0 for gRNA in E2 module (5' CCAT-3' ACTA)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU6-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKE013

    Plasmid

    #207916

    Purpose

    Level 0 for gRNA in E1 module (5' CTGC-3' CCAT)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU3-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSIBR002

    Plasmid

    #177665

    Purpose

    SIBR Intron 2 (Int2) interrupts the CDS of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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