We narrowed to 862 results for: BirA

1. pLinker-BirA-3xHA-HXGPRT-loxP

   Plasmid

   #86668

   Purpose

   for generation of BirA tagged strains using CRISPR tagging technology

   Depositor

   David Sibley

   Insert

   Linker-BirA-3xHA-HXGPRT 3'UTR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA_FRT_SNCA-Avi_HygR-2A-BirA.dna(TO_deleted). (pEHA1448)

   Plasmid

   #209083

   Purpose

   SNCA (Avi tagged) expression along with BirA ligase

   Depositor

   Vikram Khurana

   Article

   Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

   Inserts

   SNCA (SNCA Human)
   BirA
   

   Use

   Tags

   Avi

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CMV

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

   Plasmid

   #79887

   Purpose

   Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMT-ubb-NLS-BirA-2a-mCherry

   Plasmid

   #79886

   Purpose

   Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT-MCS-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80059

   Purpose

   MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS

   Plasmid

   #127365

   Purpose

   Binary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plants

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   BirA* (R118G mutant)
   

   Use

   Tags

   GS linker, NLS, V5, and YFP

   Expression

   Plant

   Mutation

   R118G

   Promoter

   Arabidopsis UBQ10 promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-BirA-FLAG-N-term-HDLBP

   Plasmid

   #154453

   Purpose

   Expression of N-term BirA tagged HDLBP

   Depositor

   Markus Landthaler

   Article

   Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

   Insert

   HDLBP (HDLBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3/flag-pBirA(1-G78)-FRB

   Plasmid

   #154447

   Purpose

   expresses pBirA(1-G78)-FRB/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   flag-pBirA(1-G78)-FRB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMT-zic2a-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80065

   Purpose

   Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Zic2a enhancer driving c-fos promoter

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. R4pGWB601_UBQ10p-BirA(R118G)-NES-YFP

    Plasmid

    #127363

    Purpose

    Binary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Use

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Plant

    Mutation

    R118G

    Promoter

    Arabidopsis UBQ10 promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-MCS-BirA(R118G)-HA-MACROD1

    Plasmid

    #172581

    Purpose

    For transient expression of C-terminal BirA-tagged MACROD1

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD1 (MACROD1 Human)
    

    Use

    Tags

    BirA(R118G)-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT-myl7-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80061

    Purpose

    Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Myl7 promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLPC-N-Myc-Flag-BirA-hPOT1

    Plasmid

    #166409

    Purpose

    express BirA-hPOT1 in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    hPOT1 (POT1 Human)
    

    Use

    Tags

    BirA, flag, and myc

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXL-CAG-mBirA272-NRX3b D137A

    Plasmid

    #43922

    Depositor

    Alice Ting

    Article

    Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

    Insert

    BirA272-NRX3b (birA Synthetic, Human)
    

    Use

    Tags

    2XFLAG

    Expression

    Mammalian

    Mutation

    D137A

    Promoter

    CAG

    Available since

    April 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS416-ADH1p-BirA-FRB-HA3

    Plasmid

    #227984

    Purpose

    Expresses BirA-FRB fusion from ADH1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    BirA-FRB
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS416-ADH1p-BirA-FKBP-HA3

    Plasmid

    #227986

    Purpose

    Expresses BirA-FKBP fusion from ADH1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    BirA-FKBP
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pME-myc-BirA (JDW 1210)

    Plasmid

    #224509

    Purpose

    Gateway compatible middle entry clone containing a Myc tagged BirA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Myc-BirA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-Myc-BirA*-RNF8-C403S

    Plasmid

    #221685

    Purpose

    Mammalian expression of Myc-tagged BirA*-RNF8 (C403S) fusion protein for BioID

    Depositor

    Derrick Sek Tong Ong

    Article

    Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

    Insert

    RNF8 (RNF8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C403S

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-Myc-BirA*-RNF8-R42A

    Plasmid

    #221684

    Purpose

    Mammalian expression of Myc-tagged BirA*-RNF8 (R42A) fusion protein for BioID

    Depositor

    Derrick Sek Tong Ong

    Article

    Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

    Insert

    RNF8 (RNF8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R42A

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-WT

    Plasmid

    #218834

    Purpose

    Expresses N-terminal BirA*-tagged human wild type TLK1 variant 5 for BioID experiments.

    Depositor

    Travis Stracker

    Insert

    TLK1 (TLK1 Human)
    

    Use

    Tags

    FLAG-BirA*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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