We narrowed to 5,324 results for: cycs

1. pF1-Hs Cdk1/cyclin B1/Cks1

   Plasmid

   #177329

   Purpose

   Co-expression of the human active Cdk1/cyclin B1/Cks1 kinase complex

   Depositor

   Andreas Boland

   Article

   Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

   Inserts

   Cyclin-dependent kinase 1 (CDK1 Human)
   G2/mitotic-specific cyclin-B1 (CCNB1 Human)
   Cyclin-dependent kinases regulatory subunit 1 (CKS1B Human)
   

   Use

   Tags

   2 x Strep-tag and 8 x His

   Expression

   Insect

   Mutation

   Promoter

   p10 and polh

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. p415-GalL-Cas9-CYC1t

   Plasmid

   #43804

   Purpose

   A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

   Depositor

   George Church

   Article

   Dicarlo et al Nucleic Acids Res

   Insert

   Human Optimized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GalL

   Available since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. D. vulgaris sp2 CRISPR/pACYCDuet-1

   Plasmid

   #81186

   Purpose

   Expresses CRISPR array containing 3 copies of Desulfovibrio vulgaris spacer 2 and flanking repeats in E. coli

   Depositor

   Jennifer Doudna

   Article

   Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

   Insert

   Synthetic CRISPR array (3 copies of D. vulgaris spacer #2 flanked by repeats)
   

   Use

   CRISPR

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACYC-T7-Nla-repeat-BbsI

   Plasmid

   #180215

   Purpose

   For cloning Neisseria lactamica Type I-C crRNA for protein purification in E.Coli

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLIB-HIS-TEV-Cyclin-B

   Plasmid

   #177012

   Purpose

   Generate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEV

   Depositor

   Andrea Musacchio

   Article

   Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

   Insert

   Cyclin B1 (CCNB1 Human)
   

   Use

   Tags

   Polyhistidine-TEV

   Expression

   Insect

   Mutation

   codon-optimised for insect cell expression

   Promoter

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

   Plasmid

   #83946

   Purpose

   Cas9-carrying vector with kanMX marker

   Depositor

   Irina Borodina

   Article

   Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX 6P1 Cyclin A (555)

   Plasmid

   #61844

   Purpose

   bacterial expression of GST cyclin A

   Depositor

   Jonathon Pines

   Insert

   cyclin A (CCNA2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   L2V

   Promoter

   tac

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pLVX-TetOne-FFSS-Cyclin D1

   Plasmid

   #215146

   Purpose

   Expresses FFSS-Cyclin D1 in mammalian cells from an inducible lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   FFSS (FLAG-FLAG-STREP-STREP)

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. CycA-Venus-Flag (1305)

   Plasmid

   #39853

   Depositor

   Jonathon Pines

   Article

   Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

   Insert

   Cyclin A (CCNA2 Human)
   

   Use

   Tags

   Flag and Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

10. 3xFLAG-dCas9/pTEF1p-CYC1t

    Plasmid

    #62190

    Purpose

    Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Yeast

    Mutation

    human codon-optimized, D10A, H840A

    Promoter

    TEF1 promoter

    Available since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-FFSS-Cyclin D1 (T286A)

    Plasmid

    #215152

    Purpose

    Expresses FFSS-Cyclin D1 in mammalian cells from a lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    FFSS (FLAG-FLAG-STREP-STREP)

    Expression

    Mammalian

    Mutation

    With T286A mutation.

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV Flag cycT2b (P#1718)

    Plasmid

    #14599

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

    Insert

    cycT2 (CCNT2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    A137T compared to NP_490595.1

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC002 - LshC2C2 locus into pACYC184

    Plasmid

    #79151

    Purpose

    Endogenous LshC2c2 locus cloned into pACYC184

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    LshC2c2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF myc cycT2b (P#1717)

    Plasmid

    #14598

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

    Insert

    cycT2 (CCNT2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pFLEX-m-cyclin D1 -T286A mutant

    Plasmid

    #86792

    Purpose

    mammalian expression of cyclin D1 T286A mutant

    Depositor

    Martine Roussel, Charles Sherr

    Article

    Matsushime et al Cell. 1991 May 17;65(4):701-13.

    Insert

    cyclin D1 (Ccnd1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T286A

    Promoter

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. CUP Cyc8 YFP p316

    Plasmid

    #15594

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    CYC8 (CYC8 Budding Yeast)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pDZ251 (pKAN 24xPP7SL cyc)

    Plasmid

    #35192

    Depositor

    Robert Singer

    Article

    Larson et al Science. 2011 Apr 22;332(6028):475-8.

    Insert

    24xPP7SL cyc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIS1 Cyclin D2 long UTR

    Plasmid

    #21645

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Cyclin D2 long UTR (CCND2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVenus-CyclinB1(R42A) (1317)

    Plasmid

    #39873

    Depositor

    Jonathon Pines

    Article

    Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

    Insert

    CyclinB1(R42A) (CCNB1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    R42A = destruction box mutations = non-destructib…

    Promoter

    Available since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

20. PSUC2-GFPantisense-CYC1t-pRS413

    Plasmid

    #64404

    Purpose

    SUC2 regulated expression of an antisense RNAi construct for GFP

    Depositor

    Claudia Vickers

    Article

    Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

    Insert

    GFPantisense
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SUC2

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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