We narrowed to 747 results for: streptococcus -(crispr) -(cas9)

1. pBBR_Cas9_sp1

   Plasmid

   #154851

   Purpose

   Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome

   Depositor

   John van der Oost

   Article

   Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.

   Inserts

   Spcas9 gene
   sgRNA scaffold
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Mutation

   Codon harmonized variant for expression in Rhodob…

   Promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEN019-SpCas9-hiNLS-t-M1M4

   Plasmid

   #234456

   Purpose

   Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

   Depositor

   Ross Wilson

   Article

   Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

   Insert

   SpCas9 hiNLS t-M1M4
   

   Use

   CRISPR

   Tags

   His6, Nucleoplasmin, SV40, and c-Myc

   Expression

   Bacterial

   Mutation

   Cys at positions 80 and 574 have been mutated to …

   Promoter

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #64324

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-(BbsI)_CBh-Cas9-T2A-BFP

   Plasmid

   #64323

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-EBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available Since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3xFLAG-dCas9/pMSCVhyg

   Plasmid

   #134323

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS415_pGal-nCas9(D10A)

   Plasmid

   #79618

   Purpose

   Expresses nCas9(D10A) in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   D10A for nickase Cas9

   Promoter

   pGal1

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3xFLAG-dCas9/pMSCVneo

   Plasmid

   #134982

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3xFLAG-dCas9/pMSCVpuro

   Plasmid

   #134983

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS415_pGal-nCas9(H840A)

   Plasmid

   #79616

   Purpose

   Expresses nCas9(H840A) in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H840A for nickase Cas9

   Promoter

   pGal1

   Available Since

   Sept. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-Cas9-tdTomato

    Plasmid

    #141013

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA

    Plasmid

    #199254

    Purpose

    Expression construct encoding the high specificity SpCas9-KARA-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    High specificity S. pyogenes Cas9-D10A-KARA nickase
    

    Use

    CRISPR

    Tags

    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A K848A R1060A

    Promoter

    CAG promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHiFi Cas9-2×sgRNA (empty, donor)

    Plasmid

    #162277

    Purpose

    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.

    Depositor

    Kazuhisa Nakayama

    Article

    Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi

    Insert

    HiFi Cas9
    

    Use

    CRISPR

    Tags

    BPNLS and FLAG tag

    Expression

    Mammalian

    Mutation

    SpCas9 (R691A)

    Promoter

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. AV15_pCAG.Cas9.gRNA.S1

    Plasmid

    #199259

    Purpose

    Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Inserts

    SpCas9 nuclease
    AAVS1-targeting gRNA
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter and RNA polymerase III promoter for …

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. AV44_pCAG.Cas9-D10A.gRNA.S1

    Plasmid

    #199258

    Purpose

    Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Inserts

    SpCas9-D10A nickase
    AAVS1-targeting gRNA
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG promoter and RNA polymerase III promoter for …

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMC-6-AtSpCas9-9

    Plasmid

    #197778

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    AtSpCas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AV13_pCAG.Cas9.gRNA.NT

    Plasmid

    #199260

    Purpose

    Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Inserts

    SpCas9 nuclease
    Non-targeting (NT) control gRNA
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter and RNA polymerase III promoter for …

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-2NLS-Cas9

    Plasmid

    #229773

    Purpose

    Expresses Cas9 driven by a CMV promoter

    Depositor

    Yujia Cai

    Article

    Ling et al Nat Nanotechnol. 2025 Apr;20(4):543-553.

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKMW123_SpRY-Cas9_U6-entry

    Plasmid

    #244820

    Purpose

    Mammalian expression of SpRY Cas9 with Golden Gate-compatible sgRNA spacer cassette

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    SpRY Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG-SV40 NLS and Nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

    Promoter

    CAG, U6

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT3TS-Hypa-nCas9n-A69

    Plasmid

    #125645

    Purpose

    micro-injection of poly-adenylated Hypa-nCas9n RNA

    Depositor

    Jason Berman

    Article

    Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

    Insert

    Hypa-nCas9n
    

    Use

    Rna in vitro transcription vector

    Tags

    Expression

    Mutation

    N692A, M694A, Q695A, H698A from wild-type Cas9

    Promoter

    T3

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

    Plasmid

    #64216

    Purpose

    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA targeting ROSA26-1
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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