We narrowed to 165,918 results for: addgene

1. MXS_CMV::PuroR-bGHpA

   Plasmid

   #62439

   Purpose

   MXS_chaining vector with CMV::PuroR-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   resistance cassette against Puromycin with CMV Promoter
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. WIPI2 sgRNA

   Plasmid

   #207554

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the WIPI2 locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   CGCGCGCCCAGCCATGAACC
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNR64

   Plasmid

   #79540

   Purpose

   Input plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.

   Depositor

   Timothy Lu

   Article

   Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

   Inserts

   TP901
   BxbI
   

   Tags

   His tag and LAA degradation tag

   Expression

   Bacterial

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pL1P5 ZmUbiP:GRF-GIF:NosT

   Plasmid

   #198046

   Purpose

   MoClo Golden Gate Level 1 Position 5. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

   Depositor

   John Innes Centre - Wheat Transformation and Genome Editing, Cristobal Uauy

   Insert

   ZmUbiP::TaGRF4-GIF1::NosT
   

   Use

   Synthetic Biology

   Promoter

   Zea mays (maize) ubiquitin promoter

   Available Since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIgnaviCas9

   Plasmid

   #127595

   Purpose

   Expresses IgnaviCas9 in E. coli

   Depositor

   Stephen Quake

   Article

   Schmidt et al Proc Natl Acad Sci U S A. 2019 Oct 28. p

   Insert

   IgnaviCas9
   

   Tags

   6xHis Tag and Maltose binding protein

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHPyV7-713a

   Plasmid

   #24728

   Depositor

   Christopher Buck

   Article

   Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

   Insert

   Full genome of Human polyomavirus 7
   

   Use

   Viral clone

   Available Since

   June 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. ICP8-P2A/Crimson (pSLIK4)

   Plasmid

   #113859

   Purpose

   For Doxycycline-inducible expression of ICP8 synaptase gene, with ICP8-P2A-red fluorescent protein gene E2-Crimson

   Depositor

   Richard S Myers

   Article

   Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

   Insert

   ICP8
   

   Use

   Lentiviral

   Tags

   P2A/Crimson

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p8389 LentiCRISPRv2 Neo sgNT-1

   Plasmid

   #221649

   Purpose

   Expression of non-targeting control sgRNA

   Depositor

   Elizabeth White

   Article

   Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

   Insert

   non-targeting sgNT-1
   

   Use

   CRISPR and Lentiviral

   Available Since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBEVY-T

   Plasmid

   #51232

   Purpose

   bi-directional expression vector for yeast, constitutively active, TRP1 selection

   Depositor

   Charles Miller

   Article

   Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   GPD/ADH1

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHPyV6-607a

    Plasmid

    #24727

    Depositor

    Christopher Buck

    Article

    Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

    Insert

    Full genome of Human polyomavirus 6
    

    Use

    Viral clone

    Available Since

    June 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDB4280

    Plasmid

    #98699

    Purpose

    A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p184_LTJ_sgRNACD90.2

    Plasmid

    #82670

    Purpose

    sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting mouse CD90.2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUG+p97W (C2)

    Plasmid

    #74018

    Purpose

    lentiviral expression of EGFP-SAP97beta fusion

    Depositor

    Oliver Schluter, Weifeng Xu, Robert Malenka

    Article

    Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

    Insert

    SAP97 beta isoform
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiMutB3GNT5-blast

    Plasmid

    #208380

    Purpose

    lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    nt 922 c->g (PAM site inactivation)

    Promoter

    EF-1 alpha core promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDB4281

    Plasmid

    #98700

    Purpose

    A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. 53BP1 N-terminal sgRNA

    Plasmid

    #207091

    Purpose

    pX330 based plasmid for expression of Cas9 and the GGGGAGCAGATGGACCCTAC sgRNA to target the 53BP1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GGGGAGCAGATGGACCCTAC
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPR_eGFP_497

    Plasmid

    #111824

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 477-497
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. p-dCas9-LSD1-K661A-Hygro

    Plasmid

    #104414

    Purpose

    transient expression of dCas9-dLSD1 fusion protein

    Depositor

    Stephan Beck

    Insert

    dLSD1 (KDM1A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    IRATp970A0364D (Source Bioscience) has a P405H ch…

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. ATG5 sgRNA

    Plasmid

    #207555

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ATG5 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    AACTTGTTTCACGCTATATC
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLJM1-PCYT2

    Plasmid

    #81074

    Purpose

    Expresses PCYT2 in mammalian cells

    Depositor

    Erin O'Shea

    Article

    Chidley et al Elife. 2016 Jul 12;5. pii: e14601. doi:

    Insert

    phosphate cytidylyltransferase 2, ethanolamine (PCYT2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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