We narrowed to 17,901 results for: REV

1. pSMT-MjtRNA-GFP(47TAG)

   Plasmid

   #193251

   Purpose

   Expresses the M. jannaschii tRNATyr (MjtRNA) and GFP(47TAG) (aka GFP151TAG)

   Depositor

   Peter Schultz, Jessica Seeliger

   Article

   Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

   Inserts

   GFP(47TAG)
   MjtRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon for Tyr47 replaced with amber stop codon TA…

   Promoter

   hsp60 and metU

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS416-Gal4-dCas9-VP64

   Plasmid

   #71128

   Purpose

   This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.

   Depositor

   Ronald Davis

   Article

   Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.

   Insert

   Gal4-dCas9-VP64
   

   Use

   Tags

   NLS n-terminal, N terminal Gal4 Activator domain,…

   Expression

   Yeast

   Mutation

   Promoter

   Tef1

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-deltaPEST

   Plasmid

   #221118

   Purpose

   FAP tagged STE3-PEST mutant (delta413-470 - N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   MFA-IgKappa-FAPoptim-STE3-deltaPEST
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   STE3 mutant that lacks the PEST domain in the C-t…

   Promoter

   STE3

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNK845

   Plasmid

   #219746

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Pichia pastoris, Homo sapiens

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mutant of fungal luciferase
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mutation

   I3S, N4T, F11L, I63T, T99P, T192S, A199P

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AIMTOR A757

   Plasmid

   #140829

   Purpose

   AIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757

   Depositor

   Anne Bonnieu

   Article

   Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

   Insert

   AIMTOR A757 (ULK1 Human)
   

   Use

   Tags

   NES Nuclear export signal

   Expression

   Mammalian

   Mutation

   The insert comprises only a small sequence of hum…

   Promoter

   Available since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Anti-Glypican 1 (GPC1) [IPI-GPC1.21] (Recombinant Antibody)

   Antibody

   #222511-rAb

   Purpose

   Anti-Glypican 1 (human) chimeric recombinant antibody with fused human variable and rabbit constant domains; specific for human GPC1. Does not cross-react with other GPCs.

   Depositor

   Institute for Protein Innovation (IPI)

   Article

   Institute for Protein et al unpublished

   Recommended Applications

   Flow Cytometry and Immunocytochemistry

   Reactivity

   Human

   Source Species

   Rabbit

   Isotype

   IgG1

   Trial Size

   Not available to purchase

   Available since

   Nov. 5, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

7. Anti-Glypican 1 (GPC1) [IPI-GPC1.44] (Recombinant Antibody)

   Antibody

   #222512-rAb

   Purpose

   Anti-Glypican 1 (human) chimeric recombinant antibody with fused human variable and rabbit constant domains; specific for human GPC1. Does not cross-react with other GPCs.

   Depositor

   Institute for Protein Innovation (IPI)

   Article

   Institute for Protein et al unpublished

   Recommended Applications

   Flow Cytometry and Immunocytochemistry

   Reactivity

   Human

   Source Species

   Rabbit

   Isotype

   IgG1

   Trial Size

   Not available to purchase

   Available since

   Nov. 5, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

8. AIMTOR T757

   Plasmid

   #140828

   Purpose

   AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells

   Depositor

   Anne Bonnieu

   Article

   Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

   Insert

   AIMTOR T757 (ULK1 Human)
   

   Use

   Tags

   NES Nuclear export signal

   Expression

   Mammalian

   Mutation

   The insert comprises only a small sequence of hum…

   Promoter

   Available since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1+ Flag-RAP2A

   Plasmid

   #128099

   Purpose

   Expresses RAP2A in mammalian cells in a G418-selectable plasmid

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1/TO-myc-BirA*-cCE

    Plasmid

    #112713

    Purpose

    For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

    Insert

    Rngtt (Rngtt Mouse)
    

    Use

    Tags

    BirA* (BirA R118G), HIV Rev nuclear localization …

    Expression

    Mammalian

    Mutation

    Silent mutation at Arg 132 (CGT to CGC), deleted …

    Promoter

    CMV

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. AA320

    Plasmid

    #215952

    Purpose

    Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    rev{U6_v1; DR_v0 [EnAs]; sgCD47_v1 [EnAs]; DR_v1 [EnAs]; sgCD47_v2 [EnAs]}
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AA315

    Plasmid

    #215951

    Purpose

    Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control lethal

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    rev{U6_v1; DR_v0 [EnAs]; sgPLK1_v1 [EnAs]; DR_v1 [EnAs]; sgPLK1_v2 [EnAs]}
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AA324

    Plasmid

    #215953

    Purpose

    Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    rev{U6_v1; DR_v0[EnAs]; sgCD46_v4[EnAs]; DR_v1[EnAs]; sgCD47_v2[EnAs]; DR_v2[EnAs]; sgCD55_v4[EnAs];DR_v3[EnAs];sgCD81_v4[EnAs]}
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AA083

    Plasmid

    #215934

    Purpose

    Fragmid fragment: (guide cassette) reverse orientation; guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    rev{U6_v1; DR_v0 [EnAs]; sgCD4_v1 [EnAs]; DR_v1 [EnAs]; sgCD4_v2 [EnAs]}
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4/TO-bio-myc-cCE

    Plasmid

    #112711

    Purpose

    For tetracycline-inducible expression of bio-myc-NES-mCE ΔNLS ("bio-myc-cCE") in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

    Insert

    Rngtt (Rngtt Mouse)
    

    Use

    Tags

    HIV Rev nuclear localization signal (NES), Stop c…

    Expression

    Mammalian

    Mutation

    Silent mutation at Arg 132 (CGT to CGC), deleted …

    Promoter

    CMV with Tet repressor binding sites

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213027

    Purpose

    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    D702Y (RT- reverse transcriptase catalytic dead)

    Promoter

    CMV

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. dRMCE Plasmids

    Plasmid Kit

    #1000000015

    Purpose

    Takes advantage of wild-type loxP and FRT sites present in these conditional alleles in mouse embryonic stem cells to introduce tags, reporters, and mutant coding regions into an endogenous loci

    Depositor

    Rolf Zeller

    Available since

    July 16, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pFa6a MET25

    Plasmid

    #61913

    Purpose

    Expression of S. cerevisiae MET25 cloned into pFA6a

    Depositor

    Jon Houseley

    Insert

    MET25
    

    Use

    Yeast genomic targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. TY5_hisAI_2micron

    Plasmid

    #138622

    Purpose

    Ty5 restrotransposon with a reversed artificial intron used for Calling Cards experiment

    Depositor

    Rob Mitra

    Insert

    TY5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1_10

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. P2r-P3 Ste3pmCherry

    Plasmid

    #58561

    Purpose

    pDONR P2r-P3 carrying the STE3 promoter mCherry fusion (reverse orientation)

    Depositor

    Leonid Kruglyak

    Insert

    Ste3promoter-mCherry
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only