We narrowed to 6,485 results for: plasmid dna

1. pET-Bxb1-His

   Plasmid

   #123132

   Purpose

   Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.

   Depositor

   David Liu

   Article

   Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

   Insert

   Bxb1 integrase
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-scFvGCN4sfGFPTET1CD

   Plasmid

   #82561

   Purpose

   Expression vector for antibody-sfGFP-TET1CD.

   Depositor

   Izuho Hatada

   Article

   Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

   Insert

   scFvGCN4sfGFPTET1CD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-C1-FLAG-Ku80

   Plasmid

   #46958

   Purpose

   Plasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   Ku80 (XRCC5 Human)
   

   Use

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHelper_ShCAST_sgRNA

   Plasmid

   #127921

   Purpose

   Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRel

   Plasmid

   #227581

   Purpose

   Release plasmid with Cas9 working cassette, apramycin resistance, and pSG5 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. rs214-minor

   Plasmid

   #113380

   Purpose

   evaluate FOXA1 DIV motif enhancer activity in SNP rs2149943 minor allele (T)

   Depositor

   Ralf Jauch

   Article

   Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

   Insert

   rs2149943 minor allele
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-C1-FLAG-Ku70

   Plasmid

   #46957

   Purpose

   Plasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   Ku70 (XRCC6 Human)
   

   Use

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCap-101-Hgy

   Plasmid

   #227508

   Purpose

   Capture plasmid with hygromycin resistance and pIJ101 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRel-Hyg

   Plasmid

   #227582

   Purpose

   Release plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSUP-(2xCouRS-tRNAopt)

    Plasmid

    #197930

    Purpose

    Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

    Depositor

    Kenneth Johnson

    Article

    Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

    Inserts

    CouRS
    tRNA opt
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    glnS' and lambda(ts) and proK

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHelp

    Plasmid

    #227583

    Purpose

    Accessory plasmid with a sgRNA cassette for Cas9, and ampicillin resistance

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Insert

    guide RNA scaffold
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pgRNA-modified

    Plasmid

    #84477

    Purpose

    guide RNA expression lentiviral construct

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    guide RNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9n(BB) (PX460)

    Plasmid

    #48873

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. Bxb1-GA_AIO-TetOn_mScarlet_Divergent

    Plasmid

    #229791

    Purpose

    Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Bxb1-GT_CAG_3xTurboGFP-NLS

    Plasmid

    #229777

    Purpose

    Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP (3 copies)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    3x TurboGFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMBP-SpyCatcher

    Plasmid

    #79053

    Purpose

    Expresses maltose binding protein (MBP) SpyCatcher fusion

    Depositor

    James Bowie

    Article

    Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

    Insert

    MBP-SpyCatcher
    

    Use

    Tags

    6xHis and Maltose binding protein

    Expression

    Bacterial

    Mutation

    Derived from pDEST14-SpyCatcher (Plasmid #35044)…

    Promoter

    T7

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUCmEos3.2:cat

    Plasmid

    #78100

    Purpose

    Plasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.

    Depositor

    Peng Chen

    Article

    Chen et al Nat Commun. 2015 Jul 6;6:7445. doi: 10.1

    Insert

    mEos3.2-cat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

18. Bxb1-GA_CAG-TurboGFP

    Plasmid

    #229781

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TurboGFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. AX53_pS.Donor.S1.TS

    Plasmid

    #100290

    Purpose

    AAVS1-targeting plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    18 bp RFLP (MluI+KspAI)
    

    Use

    CRISPR and Synthetic Biology; Human targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLPC31

    Plasmid

    #209996

    Purpose

    Contains Level 0 Part: 3' Connector (3C2SN) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    3' Connector (3C2SN)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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