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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,812 results for: cloning

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Showing: 2481 - 2500 of 63812 results

  4. DNMT3A-dCas9

    Plasmid
    #100090
    Purpose
    DNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNMT3A (DNMT3A Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-DNMT3A-dCas9-NLS
    Expression
    Mammalian
    Mutation
    includes aa 602-912
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100090-plasmid-map-sequence-id-197562

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  5. pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40

    Plasmid
    #101064
    Purpose
    AAV vector expressing CaMPARI2_L398T (Kd = 825nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons
    Depositor
    Eric Schreiter
    Publication
    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:
    Has service
    AAV1
    Insert
    CaMPARI2_L398T
    Use
    AAV
    Tags
    FLAG-HA-myc and NES_his
    Promoter
    hsyn (synapsin-1)
    Available since
    Oct. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101064-plasmid-map-sequence-id-194371

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  9. BPK2139

    Plasmid
    #65776
    Purpose
    Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65776-plasmid-map-sequence-id-112651

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  10. M_ST1n_VPR

    Plasmid
    #63799
    Purpose
    ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector
    Depositor
    George Church
    Publication
    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ST1-dCas9-VPR
    Use
    CRISPR and Synthetic Biology
    Tags
    VPR (VP64-p65-Rta)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    63799-plasmid-map-sequence-id-106659

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  12. pACT2-CAS9c

    Plasmid
    #51055
    Purpose
    Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.
    Depositor
    Yunde Zhao
    Publication
    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1
    Insert
    Cas9c
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Bacterial and Yeast
    Promoter
    yeast ADH1 promoter
    Available since
    Feb. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51055-plasmid-map-sequence-id-77327

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  16. pLentiN

    Plasmid
    #37444
    Purpose
    Control lentiviral plasmid containing FLAG-HA tags
    Depositor
    Karl Munger
    Publication
    Spangle et al J Virol. 2012 May 2.
    Insert
    FLAG-HA
    Use
    Lentiviral
    Tags
    Flag and HA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 3, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 2481 - 2500 of 63812 results