We narrowed to 17,413 results for: She
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Plasmid#37404PurposeThis is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.DepositorInsertCre Shine
UseCre/LoxTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
lenti-UCOE-dCas9-BFP-VP64
Plasmid#196717PurposeCRISPR-activation. dCas9-VP64 with TagBFP for sorting.DepositorInsertdCas9-TagBFP-VP64
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationD10A, N863APromoterEF1aAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNAI Neo mouse PECAM FL
Plasmid#42901DepositorInsertPECAM (Pecam1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
SNX32A-c014
Plasmid#175486PurposeProtein expression in bacterial cells. PX domain, S17-M197DepositorInsertSNX32 (SNX32 Human)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-GRASP65
Plasmid#137709PurposeEukaryotic expression of rat GRASP65 full-lengthDepositorInsertGRASP65 (Gorasp1 Rat)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQC V5 MCS IRES Puro
Plasmid#110342Purposeγ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Puromycin selection.DepositorTypeEmpty backboneUseRetroviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET-MBP-NLS-Geo_st
Plasmid#87703PurposeExpression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLSDepositorInsertGeoCas9
UseTags10xHis-MBP-TEVExpressionBacterialMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQXCINeo-LOXL2-BC
Plasmid#134763Purposea retroviral vector to express lysyl oxidaseL2DepositorInsertLOXL2 (LOXL2 )
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pET28-3'TwStrep
Plasmid#208053PurposeEmpty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.DepositorTypeEmpty backboneUseTagsTwin StrepExpressionBacterialMutationPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX404 Campylobacter jejuni Cas9
Plasmid#68338PurposeCampylobacter jejuni Cas9DepositorInsertCjCas9
UseCRISPRTagsHA-NLS and NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-5'TwStrep
Plasmid#208620PurposeEmpty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.DepositorTypeEmpty backboneUseTagsTwin StrepExpressionBacterialMutationPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS303-PADH1-AFB2
Plasmid#99530PurposeS. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, HIS3 marker (for use with the AID degron system)DepositorInsertAFB2 (AFB2 Mustard Weed)
UseTagsExpressionYeastMutationPromoterADH1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-tMCK-mem-TurboID
Plasmid#149410PurposeMuscle-specific expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selectionDepositorInsertmem-TurboID
UseAAVTagsHA and V5ExpressionMutationPromotertMCKAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Bcl2
Plasmid#191964PurposeExogenous expression of Bcl2 WTDepositorInsertBcl2 (BCL2 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSP12::dsRed2
Plasmid#30171DepositorInsertMycobacterium Strong Promoter (MSP)
UseMycobacteria expressionTagsdsRed2ExpressionMutationPromoterAvailable sinceJune 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-Impact-T4gp43(exo-)
Plasmid#228202PurposeExpression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219ADepositorInsert43
UseTagsSce VMA intein-chitin binding domainExpressionBacterialMutationMutated aspartate 219 to alanine (D219A), elimina…PromoterT7Available sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBS L30 mRuby3-Rab10
Plasmid#166429PurposeRab10 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.DepositorInsertRab10
UseTagsmRuby3ExpressionMammalianMutationPromoterL30 weak promoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB700-mouse-TTN-Cerulean
Plasmid#128325PurposeSP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)DepositorInsertgRNA against mouse TTN for activation
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBS-L1PA1CH_blast rescue
Plasmid#69611PurposeExpresses a blasticidin tagged codon optimized L1PA1 designed to recover insertsDepositorInsertL1PA1
UseTagsblast rescue cassetteExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
DFNB31-TwinStrepII
Plasmid#83196PurposeExpression of tagged protein in mammalian cellsDepositorInsertDFNB31 (Whrn Mouse)
UseTagsTwin Strep Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hUCP4-NE
Plasmid#102362PurposeOverexpression of solute carrier family 25 member 27 in mammalian cellsDepositorInsertsolute carrier family 25 member 27 (SLC25A27 Human)
UseTagsNEExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hSyncytin-1(del480)
Plasmid#182441PurposehSyncytin-1, deletion 481-538DepositorInsertERVW-1 (ERVW-1 Human)
UseTagsExpressionMammalianMutationdeletion 481-538PromoterCMVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
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FU_tetO_dCas9-VP192-T2A-EGFP
Plasmid#121173PurposeLentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.DepositorInsertdCas9-VP192-T2A-EGFP
UseCRISPR and Lentiviral; Inducible by doxycyclineTagsT2A-EGFP-ires-puroExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)
Plasmid#51656Purposehsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrangeDepositorInsertQuasar2-mOrange-Chr64-GFP
UseLentiviralTagsGFP and mOrangeExpressionMammalianMutationPromoterhuman synapsinAvailable sinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCer-C3-Plk1-T210D
Plasmid#68133PurposeExpression of Cerulean-tagged, constitutively active Plk1 in mammalian cellsDepositorInsertPolo-like kinase 1 (PLK1 Human)
UseTagsCeruleanExpressionMammalianMutationT210D constitutively active versionPromoterCMVAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pXR003_puroR
Plasmid#219819PurposehU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-VP64-Hygro
Plasmid#104405Purposetransient expression of dCas9-VP64 fusion proteinDepositorInsertVP64
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX380 Treponema denticola Cas9
Plasmid#68317PurposeTreponema denticola Cas9DepositorInsertTdCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pBHM2641
Plasmid#229525PurposeCarries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseCRISPRTagst2a-mNeonGreen-AID*ExpressionBacterial and YeastMutationF74GPromoterTEF1Available sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2647
Plasmid#229989PurposeCarries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseTagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2648
Plasmid#229990PurposeCarries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseTagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2649
Plasmid#229991PurposeCarries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseTagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCer-C3-Plk1-K82R
Plasmid#68134PurposeExpression of Cerulean-tagged, kinase-dead Plk1 in mammalian cellsDepositorInsertPolo-like kinase 1 (PLK1 Human)
UseTagsCeruleanExpressionMammalianMutationK82R kinase-dead versionPromoterCMVAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
m.3047-Left TALE-G1397-N-DddA11-mCherry
Plasmid#211336PurposeExpression of codon-optimized mitochondrial base editor in human cells; cell sorting; blasticidin drug selectionDepositorInsertm.3047-Left TALE-G1397-N-DddA11-mCherry
UseTALENTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
p426TEF-LptTPS-A
Plasmid#89468PurposeExpression of prespatane synthase LptTPS-A in S. cerevisiae for sesquiterpene productionDepositorInsertLptTPS-A
UseTagsnoneExpressionYeastMutationPromoterpBR322 origin of replication for propagation in E…Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only