We narrowed to 4,207 results for: Bre;

1. pCMV-FLAG-WRAP53beta-siRNA-resistant

   Plasmid

   #64680

   Purpose

   expresses siRNA resistant WRAP53beta

   Depositor

   Marianne Farnebo

   Article

   Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

   Insert

   WD40-encoding RNA antisense to P53 (WRAP53 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   siRNA resistant

   Promoter

   CMV

   Available since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pEN_hU6miR-Gb2-K

   Plasmid

   #25759

   Purpose

   Entry vector with human U6 promoter driving both mouse G alpha 12 and G alpha 13 miR30-based shRNAs.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   Gb2 miR-shRNA (Gnb2 Mouse)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEN_TTmiRc2_BirA_NRF2

   Plasmid

   #136524

   Purpose

   Used as a donor vector to clone into pSLIK

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   NRF2 (NFE2L2 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMVA2RBS035

   Plasmid

   #121051

   Purpose

   Expresses recombinant MVA pathway in Escherichia coli

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

   Inserts

   mvaE
   mvaS
   mvaK1
   mvaK2
   mvaD
   idi
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-HA-ER WT

   Plasmid

   #49498

   Purpose

   Expresses HA-tagged ER wild-type in mammalian cells

   Depositor

   Sarat Chandarlapaty

   Article

   Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

   Insert

   ESR1 (ESR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-RIGI-Apex- T2A-EGFP

   Plasmid

   #167288

   Purpose

   Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   DDX58 (RIGI Human)
   

   Use

   Lentiviral

   Tags

   APEX2 and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. tet-pLKO-sgRNA-puro

   Plasmid

   #104321

   Purpose

   3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.

   Depositor

   Nathanael Gray

   Article

   Huang et al eLife. 2017 Sep 19;6. pii: e26693. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1/TO

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-H2B-tdTomato-SRT

   Plasmid

   #203393

   Purpose

   AAV transfer plasmid containing H2B fused piggyBac self-reporting transposon with tdTomato marker for mammalian calling cards

   Depositor

   Joseph Dougherty, Rob Mitra

   Article

   Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

   Insert

   H2B fused piggyBac tdTomato self-reporting transposon
   

   Use

   AAV

   Tags

   3X SV40 and H2B

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-PE2

   Plasmid

   #132775

   Purpose

   Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   PE2
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   CMV

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. PARP1 FL

    Plasmid

    #169815

    Purpose

    Expresses codon optimised full length His tagged PARP1 in bacterial cells

    Depositor

    David Neuhaus

    Article

    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

    Insert

    Poly[ADP-ribose] polymerase 1 (PARP1 Synthetic, Codon optimised, Human)
    

    Use

    Tags

    MKHHHHHHMKQ

    Expression

    Bacterial

    Mutation

    Valine 762 mutated to an alanine

    Promoter

    T7 promoter

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-PE2-P2A-GFP

    Plasmid

    #132776

    Purpose

    Prime editing in mammalian cells with co-translational GFP expression

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    PE2-P2A-GFP
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. P-Ef1a-RPL22-3XFlag-P2A-EGFP-T2A-Puro

    Plasmid

    #170318

    Purpose

    Expresses FLAG and EGFP tagged RPL22, puromycin resistance

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiGuide-zeo

    Plasmid

    #160091

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and zeocin resistance marker

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-21a_3xNLS_SpCas9_protein_expression

    Plasmid

    #114365

    Purpose

    Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Wu et al Nat Med. 2019 May;25(5):776-783. doi: 10

    Insert

    3xNLS_SpCas9
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Neo

    Plasmid

    #170319

    Purpose

    Expresses HA and EGFP tagged RPL22, neomycin resistance

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. P-Ef1a-RPL22-3XFlag-P2A-mCherry-T2A-Puro

    Plasmid

    #170322

    Purpose

    Expresses FLAG and mCherry tagged RPL22, puromycin resistance

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pABpuro-BluF

    Plasmid

    #46824

    Purpose

    luciferase reporter for BmalI promoter

    Depositor

    Steven Brown

    Article

    Brown et al PLoS Biol. 2005 Oct;3(10):e338. Epub 200

    Insert

    Bmal1 (Bmal1 Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Bmal1

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. Anti-GFP [N86/38.1R]

    Plasmid

    #114492

    Purpose

    Mammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2 LRP6-eGFP

    Plasmid

    #180143

    Purpose

    to make movies of LRP6 vesicles

    Depositor

    Edward De Robertis

    Article

    Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

    Insert

    Human LRP6 (LRP6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSCV_IRES_GFP_CD16a

    Plasmid

    #196189

    Purpose

    Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

    Depositor

    Ana Ruiz-Saenz

    Article

    Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

    Insert

    CD16a (FCGR3A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Phenylalanine 158 to Valine

    Promoter

    LTR

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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