We narrowed to 10,124 results for: Met
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Plasmid#171800PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-T2A-PuroR
UseGene taggingMutationPuroR: Changed Methionin 1 to Glycine.Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWAP-HGF
Plasmid#83503PurposeGeneration of transgenic mice overexpressing the HGF under the control of the WAP gene promoterDepositorInsertHGF (Hgf Mouse)
UseMouse TargetingMutationalso contains beta-globin sequences from pUC198Promotermouse WAP (whey acidic protein)Available sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-His-mTET1CD∆cat
Plasmid#81054Purposebacterial expression of catalytic dead catalytic domain of murine TET1DepositorInsertTET1 (Tet1 Mouse)
Tags6HISExpressionBacterialMutationcatalytic domain amino acid 1367-2057 with histid…PromoterT7Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAWPSG-Ptet-nCas9-BE
Plasmid#195739PurposeExpress APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coliDepositorPublicationInsertTet repressor protein / APOBEC1-nCas9(D10A)-UGI
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPtet(Tetracycline inducible protein)Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJE-Ma pylRS nitroY/haloY-F5
Plasmid#225684PurposeExpresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.DepositorInsertsM. alvus PylRS 3-NY/HaloY F5
M. alvus Pyl-tRNA(6)
UseMethanomethylophilus alvusMutationCTG125CTT, N166A, V168S, A223C, W239RPromoterGlnS and LppAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB4279
Plasmid#98698PurposeA control plasmid containing the intact bsdMX markerDepositorInsertCas9
UseCRISPRExpressionYeastAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPbU6_hdhfr/yfcu_Cas9
Plasmid#216423PurposeEmpty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.DepositorPublicationTypeEmpty backboneUseCRISPR; Expression in plasmodium bergheiExpressionBacterialAvailable sinceApril 28, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKMV-VioB
Plasmid#65348Purposesysthesis of vioB gene in the violacein pathwayDepositorInsertvioB
UseSynthetic BiologyAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKMV-VioE
Plasmid#65351Purposesysthesis of vioE gene in the violacein pathwayDepositorInsertvioE
UseSynthetic BiologyAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEERM6 Km
Plasmid#64027PurposePlasmid for integration into sqs site of Synechocystis 6803DepositorPublicationTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPnrsBAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA
Plasmid#140014PurposePlasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
TagsFLAGExpressionMammalianMutationY126A, Y197FPromoterEF1Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pK
Plasmid#64188Purposeharbours marker gene LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCoofy35
Plasmid#43985DepositorTypeEmpty backboneTagsMBPExpressionBacterialPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
VCA
Plasmid#27792DepositorInsertVenus-Cerulean-Amber
UseStoichiometry standardExpressionMammalianAvailable sinceJune 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-human cGAS-HA
Plasmid#186896PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)
Plasmid#71213PurposedCas9 fused to BFP and the human DNMT3A catalytically inactive domainDepositorInsertdSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A
TagsdSpCas9-BFP-DNMT3A(E752A)ExpressionMammalianAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.blast-Flag-MFF-S155,172D
Plasmid#74442PurposeLentiviral expression vector containing flag tagged S155,172D MFF isoform 5 mutantDepositorInsertFlag-MFF (MFF Human)
UseLentiviralTagsFlagExpressionMammalianMutationSer 155 Asp, Ser 172 Asp (Numbering based on MFF …Available sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM41-EcLonA
Plasmid#38330DepositorInsertLon protease fragment (lon Escherichia coli)
TagsHis, MBP, and TEVExpressionBacterialMutationPolyphosphate binding fragment of Lon protease. …PromoterT7Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAL-pyk_NT
Plasmid#74072PurposepAL374 plasmid carrying the pyk (NT) sgRNA targeting the non-template strand of pyk, SpecRDepositorPublicationInsertsgRNA pyk (NT)
UseCRISPRExpressionBacterialPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCoofy16
Plasmid#43988DepositorTypeEmpty backboneTagsHis10-NusAExpressionBacterialPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only