We narrowed to 49,526 results for: ang

1. px330_Rosa_sgRNA

   Plasmid

   #97007

   Purpose

   Expresses Cas9 and Rosa26 locus specific sgRNA

   Depositor

   Russell Ray

   Article

   Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

   Insert

   Rosa26 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA Flag ERbeta

   Plasmid

   #35562

   Depositor

   Harish Srinivas

   Article

   Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

   Insert

   Estrogen Receptor beta (ESR2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRK5-Myc-Parkin

   Plasmid

   #17612

   Depositor

   Ted Dawson

   Article

   Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

   Plasmid

   #122151

   Purpose

   Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   dCas9 fused to SunTag(24x)
   

   Use

   Lentiviral

   Tags

   2xNLS-P2A-BFP and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. gRNA NIA TLS2

   Plasmid

   #196981

   Purpose

   Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   AtNIA1 gRNAs fused to TLS2 tags
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pU6-26, pU6-29

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. gRNA NIA TLS1

   Plasmid

   #196980

   Purpose

   Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   AtNIA1 gRNAs fused to TLS1 tags
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pU6-26, pU6-29

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GFP-cdc42palm

   Plasmid

   #20141

   Depositor

   Alaa El-Husseini

   Article

   Kang et al Nature. 2008 Dec 18. 456(7224):904-9.

   Insert

   cell division cycle 42 isoform 2 (CDC42 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

8. GFP-cdc42prenyl

   Plasmid

   #20142

   Depositor

   Alaa El-Husseini

   Article

   Kang et al Nature. 2008 Dec 18. 456(7224):904-9.

   Insert

   cell division cycle 42 isoform 1 (CDC42 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

9. Lyn FAK biosensor

   Plasmid

   #78299

   Purpose

   FRET biosensor. Visualization of FAK activity at lipid rafts in live cells

   Depositor

   Yingxiao Wang

   Article

   Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

   Insert

   Lyn Fak biosensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE-IRES-puro-NLS-dLwaCas13a-EGFP-NLS-3xFlag

    Plasmid

    #132407

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dLwaCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    R474A; H479A; R1046A; R1051A

    Promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Integrin β8-GFP

    Plasmid

    #205093

    Purpose

    Express GFP-tagged human integrin β8 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 8 (ITGB8 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCI-Neo-PTPRT (WT)

    Plasmid

    #16630

    Depositor

    Bert Vogelstein

    Article

    Wang et al Science. 2004 May 21. 304(5674):1164-6.

    Insert

    PTPRT (PTPRT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

13. XZ091 (PB) CMV-sCCL20 (PuroR-BFP)

    Plasmid

    #233434

    Purpose

    PiggyBac donor plasmid, secreted CCL20

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    CCL20 (CCL20 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHAGE-IRES-puro-NLS-dLbaCas13a-EGFP-NLS-3xFlag

    Plasmid

    #132408

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dLbaCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    R600A; H605A; R1243A; H1248A

    Promoter

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP-nArgBP2ΔNSE

    Plasmid

    #74515

    Purpose

    Express GFP-nArgBP2ΔNSE fusion protein in mammalian cells. nArgBP2ΔNSE is the mutant without the neuronal specific exon of nArgBP2.

    Depositor

    Guoping Feng

    Article

    Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

    Insert

    nArgBP2delNSE (Sorbs2 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-SF3B1-K700E-reporter

    Plasmid

    #200318

    Purpose

    A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.

    Depositor

    Lili Wang

    Article

    Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

    Insert

    BSD-2A-EGFPi
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Integrin β5-GFP

    Plasmid

    #205090

    Purpose

    Express GFP-tagged human integrin β5 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 5 (ITGB5 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42333

    Purpose

    This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUF1-dCas9-GCN5

    Plasmid

    #122509

    Purpose

    Combining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.

    Depositor

    Lubin Jiang

    Article

    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

    Insert

    dCas9-GCN5
    

    Use

    CRISPR

    Tags

    3*FLAG

    Expression

    Mutation

    dCas9(D10A,H840A)

    Promoter

    PfHsp86

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EFS-Vector

    Plasmid

    #109395

    Purpose

    AAV-CRISPR knockout plasmid for direct in vivo genome editing

    Depositor

    Sidi Chen

    Article

    Wang et al Sci Adv. 2018 Feb 28;4(2):eaao5508. doi:

    Type

    Empty backbone

    Use

    AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFs-NS

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. rnLbCas12a-PE2

    Plasmid

    #213746

    Purpose

    For circular RNA-mediated prime editor using rnLbCas12a-PE2 in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    rnLbCas12a-M-MLV RT∆RNase H
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R, H759A, R1138A in LbCas12a

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGP-AAV-GFAP-iGABASnFR2-WPRE

    Plasmid

    #218871

    Purpose

    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Has Service

    AAV1 and AAV5

    Insert

    iGABASnFR2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    GFAP

    Available since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSG4K5

    Plasmid

    #74492

    Purpose

    For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGP-AAV-CAG-flex-iGABASnFR2(no bind)-WPRE

    Plasmid

    #218882

    Purpose

    AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2(no bind)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    S99A F102G R168P

    Promoter

    CAG

    Available since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pM123: pAAV-EFS-CasRx-control presgRNA

    Plasmid

    #166871

    Purpose

    AAV vector for expressing CasRx and control presgRNA for RNA-editing

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Inserts

    U6-control presgRNA
    RfxCas13d
    

    Use

    AAV and CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-CAG-EGFP-P2A-FusionRed

    Plasmid

    #191108

    Purpose

    Mammalian cell line expression plasmids for production of EGFP and FusionRed proteins

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    pAAV-CAG-EGFP-P2A-FusionRed
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    chicken β-actin promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEX-gp78c/2767

    Plasmid

    #11429

    Depositor

    Allan Weissman

    Article

    Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

    Insert

    gp78c (AMFR Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    n/a

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

28. pcDNA4/TO-Flag-H3.3

    Plasmid

    #47980

    Purpose

    tetracycline-inducible expression of human Flag tagged H3F3A

    Depositor

    Bing Zhu

    Article

    Huang et al PLoS Genet. 2013 Jun;9(6):e1003558. doi:

    Insert

    H3F3A (H3-3A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    288 bp T to C synonymous mutation

    Promoter

    CMV promoter with tetracycline operator

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pSLQ1661-sgMUC4-E3(F+E)

    Plasmid

    #51025

    Purpose

    Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3

    Depositor

    Stanley Qi, Bo Huang

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    optimized sgRNA (MUC4 Mouse, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAf-CRISPR-yA

    Plasmid

    #191015

    Purpose

    CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046

    Insert

    yA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Aspergillus flavus U6

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)

    Plasmid

    #107939

    Purpose

    pie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.

    Depositor

    Heng-Chi Lee

    Article

    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

    Insert

    mCherry(dpiRNA)::ANI-1(AHPH)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    pie-1

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGP-AAV-syn-flex-iGABASnFR2n-WPRE

    Plasmid

    #218878

    Purpose

    AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2n
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    S99A F104H R168P

    Promoter

    Synapsin

    Available since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMD19_STARR-seq_plants

    Plasmid

    #117379

    Purpose

    STARR-seq vector with 35s core promoter for transient expression in plant protoplasts

    Depositor

    Xiaoyu Zhang

    Article

    Ricci et al Nat Plants. 2019 Nov 18. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Tags

    GFP derived from pMDC107

    Expression

    Plant

    Mutation

    Promoter

    35s core promoter

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AAVS1-Blasticidin-CAG-Flpe-ERT2

    Plasmid

    #68461

    Purpose

    AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection gene

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    mammalian-codon-optimized Flpe recombinase fused with ERT2
    

    Use

    Synthetic Biology

    Tags

    ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDual-IN-SMN1

    Plasmid

    #215721

    Purpose

    dual-color reporter express eGFP constitutively ; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7; expresses SMN1

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    SMN1 EXON 7 (SMN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3-Akt-STOPS-NLS

    Plasmid

    #175007

    Purpose

    Nuclear-targeted Akt-STOPS.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    mCherry-Akt-STOPS-NLS
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCDNA3-V5-PTPN14-wild type

    Plasmid

    #61003

    Purpose

    Expresses wild type human PTPN14 in mammalian cells, N-terminus V5 tag

    Depositor

    Jianmin Zhang

    Article

    Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

    Insert

    PTPN14 (PTPN14 Human)
    

    Use

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pTPH413

    Plasmid

    #185728

    Purpose

    Bacterial PAM profiling of adenine base editor variants

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Inserts

    ecTadA(8e)-neNme2-C
    Nme2Cas9 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAG-PuroDTK.Neo

    Plasmid

    #72307

    Purpose

    PiggyBac Cassette contains puromycin and TK genes

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

    Insert

    Puromycin-DTK-neo
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Feb. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRK5-HA-SLC38A9.1

    Plasmid

    #71869

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

41. Tagged-IRES

    Plasmid

    #35570

    Depositor

    Marian Waterman

    Article

    Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

    Insert

    Lymphoid Enhancer Factor 1 5'UTR (LEF1 Human)
    

    Use

    Luciferase

    Tags

    Firefly Luciferase and MS2 Stem Loop

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pENTR223-PLXNB2

    Plasmid

    #86236

    Purpose

    PLXNB2 full-length human cDNA CDS in gateway entry plasmid

    Depositor

    Roland Friedel

    Article

    Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

    Insert

    PLXNB2 (PLXNB2 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA

    Plasmid

    #100279

    Purpose

    In vitro transcription template (T7 promoter) for Kdm6b catalytic domain mutant

    Depositor

    Yi Zhang

    Article

    Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

    Insert

    Kdm6b (Kdm6b Mouse)
    

    Use

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    Mutated catalytic domain (1025aa-End) (please see…

    Promoter

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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44. pHDR-USR-SpCas9

    Plasmid

    #208829

    Purpose

    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed SpCas9. The PuroR reporter gene was designed to be repaired as a selecting marker gene only by the sgRNA/Cas9-triggered HDR.

    Depositor

    Zhiying Zhang

    Article

    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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45. plentiCMV-sct-p5-b2m-HLA-A0101

    Plasmid

    #196512

    Purpose

    Express single-chain peptide-MHC (CMV p5 epitope on HLA-A0101)

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    single chain of a CMV peptide, b2m and HLA A0101
    

    Use

    Lentiviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Y84C

    Promoter

    CMV

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. pcDNA5/FRT-mMAPT

    Plasmid

    #231584

    Purpose

    Minigene of mouse MAPT containing exon 9, 10, 11 and relevant intronic sequences

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Cell Genom. 2024 Jun 12;4(6):100563. doi

    Insert

    mouse MAPT (Mapt Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. pGEX-KG-Itch/2793

    Plasmid

    #11434

    Depositor

    Allan Weissman

    Article

    Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

    Insert

    Itch (Itch Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    n/a

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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48. pcDNA3-Akt-STOPS-NES

    Plasmid

    #175008

    Purpose

    Cytosol-targeted Akt-STOPS.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    mCherry-Akt-STOPS-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pMSCV-PIG-miR-29b-1/miR-29a

    Plasmid

    #64231

    Purpose

    Expresses miR-29b-1 and miR-29a in mammalian cells

    Depositor

    Joshua Mendell

    Article

    Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

    Insert

    miR-29b-1 and miR-29a (MIR29A, MIR29B1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCFJ150-GFP(dpiRNA)::CDK-1

    Plasmid

    #107938

    Purpose

    cdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.

    Depositor

    Heng-Chi Lee

    Article

    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

    Insert

    GFP(dpiRNA)::CDK-1 (cdk-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    cdk-1

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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