We narrowed to 10,119 results for: Met
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Plasmid#71875PurposeExpresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cellsDepositorAvailable sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by
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CMV-G-GECO1.0
Plasmid#32447PurposeMammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.0)DepositorInsertG-GECO1.0
ExpressionMammalianMutationGCaMP3 K119I/L173Q/S404G/E430VAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)
Plasmid#115698PurposeER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiaeDepositorInsertSSKar2-sfpHluorin-HDEL
TagsER retention signal containing HDEL and ER signal…ExpressionYeastMutationfor introduced mutations see publicationPromoterMET25Available sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC1-SypHer3s
Plasmid#108118PurposeRatiometric pH probe with enhanced brightness for mammalian cells expression.DepositorInsertSypHer3s
Tagsratiometric YFPExpressionMammalianPromoterCMVAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBMN FKBP(DD)-YFP
Plasmid#31763DepositorInsertFKBP(DD)-YFP-HA (FKBP1A Human)
UseRetroviralTagsHA and YFPMutationF36V, L106P on FKBP12PromoterLTRAvailable sinceOct. 3, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSMPP-mCherry-hTRIM21
Plasmid#104972PurposeLentiviral Transfer Plasmid with mCherry-tagged human TRIM21DepositorAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLIK-IDH1-FLAG
Plasmid#66802PurposeTet-inducible expression of IDH1 in mammalian cells, 3rd generation lentiviral vectorDepositorAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pgRNA-modified
Plasmid#84477Purposeguide RNA expression lentiviral constructDepositorInsertguide RNA
UseLentiviralExpressionMammalianPromotermU6Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRL623
Plasmid#58494Purposehelper plasmid for bacterial conjugal DNA transferDepositorInsertencodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120
Available sinceOct. 14, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV/SB11
Plasmid#26552DepositorPublicationInsertSleeping Beauty
ExpressionBacterialMutationMethionine 243 to Glutamine Threonine 136 to Arg…Available sinceOct. 25, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBMN YFP-DHFR(DD)
Plasmid#29326DepositorInsertYFP-DHFR(DD)
UseRetroviralTagsHA and YFPMutationChange Arg 12 to His (R12H),Asn 18 Thr (N18T), Va…Available sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEVOL-pylT-N346A/C348A
Plasmid#127411PurposeDouble mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethersDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
ExpressionBacterialMutationN346A-C348APromoteraraBADAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-ER-LAR-GECO1
Plasmid#61244PurposeExpresses LAR-GECO1 in the endoplasmic reticulum in mammalian cellsDepositorInsertLAR-GECO1
TagsER-retention sequence: KDEL and ER-targeting sequ…ExpressionMammalianMutationSubstitutions relative to R-GECO1: V51W/I113V/N35…PromoterCMVAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-FLAG LAP2
Plasmid#15738DepositorAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-CMV-CFP;UBC-Cre25nt
Plasmid#65727PurposeExpresses Cre recombinase with a 25 nucleotide extracellular vesicle targeting sequence under the UBC promotor and CFP under the CMV promotor.DepositorInsertsCMV promoter driving CFP expression
UBC promoter driving Cre-25nt expression
UseCre/LoxExpressionMammalianPromoterCMV and UBCAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40
Plasmid#101060PurposeAAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorHas serviceAAV1InsertCaMPARI2
UseAAVTagsFLAG-HA-myc and NES_hisPromoterhsyn (synapsin-1)Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP
Plasmid#85165PurposeProvides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.DepositorInsertEcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
TagsAmilCP and RTXExpressionBacterialPromoterJ23100 promoterAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-NLS-R-GECO
Plasmid#32462PurposeMammalian expression of nucleus-targeting red fluorescent genetically encoded Ca2+ indicator for optical imagingDepositorInsertR-GECO1.0
TagsNLS sequence DPKKKRKVExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMA4008
Plasmid#70109PurposemtDNA destruction/production of rho-0 cellsDepositorInsertsEGFP
Herpes simplex virus UL12.5M185
TagsnoneExpressionMammalianMutationN-terminal deletion of UL12.5PromoterCMV and RSVAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
METTL3 shRNA 2 tet pLKO puro
Plasmid#162984PurposeTet-inducible shRNA targeting human METTL3 #2DepositorInsertmethyltransferase like 3 (METTL3 Human)
UseLentiviralAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only