We narrowed to 29,277 results for: Spr

1. pDCX_mEmerald_CRISPR_HDR

   Plasmid

   #239331

   Purpose

   HDR template to insert mEmerald before the stop codon in exon 7 of the human doublecortin (DCX) locus

   Depositor

   Torsten Wittmann

   Article

   Dema et al Curr Biol. 2024 Nov 21:S0960-9822(24)014

   Insert

   mEmerald and DCX homology arms
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX459_L31_CRISPR_downstream_sgRNA

   Plasmid

   #235610

   Purpose

   Encodes CRISPR gRNA for cleaving downstream of insertion site at C terminus of mouse Rpl31

   Depositor

   Maria Barna

   Article

   Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

   Insert

   Rpl31 C-terminus downstream sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX459_L31_CRISPR_upstream_sgRNA

   Plasmid

   #235609

   Purpose

   Encodes CRISPR gRNA for cleaving upstream of insertion site at C terminus of mouse Rpl31

   Depositor

   Maria Barna

   Article

   Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

   Insert

   Rpl31 C-terminus upstream sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Shim_AspRS_3212.91_TP_GFP_pK7fWG2_IVD_FP611

   Plasmid

   #228374

   Purpose

   Expresses N-term transit peptide from Sapria himalayana Aspartate aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3212.91; determination of S. himalayana AspRS target localization

   Depositor

   Daniel Sloan

   Article

   DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   N-terminal transit peptide from S. himalayana AspRS evm.model.fragScaff scaffold 3212.91
   

   Use

   Tags

   Green Florescent Protien

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pLentiCRISPRv2_sgALDH7A1

   Plasmid

   #218273

   Purpose

   CRISPR KO plasmid for ALDH7A1 in human cell lines

   Depositor

   Seth Parker

   Article

   Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

   Insert

   Inserted sgRNA targetting ALDH7A1 (ALDH7A1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiCRISPRRv2_sgTom

   Plasmid

   #218346

   Purpose

   CRISPR KO plasmid for tdTomato in human cell lines

   Depositor

   Seth Parker

   Article

   Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

   Insert

   Inserted sgRNA targetting tdTomato
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLSPR22

   Plasmid

   #217307

   Purpose

   Promoterless (SwaI Cutsite) amdS:mRuby3 fusion reporter

   Depositor

   James Fraser

   Article

   Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

   Insert

   Acetamidase
   

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLSPR23

   Plasmid

   #217308

   Purpose

   Promoterless (SwaI Cutsite) amdS:mNeonGreen fusion reporter

   Depositor

   James Fraser

   Article

   Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

   Insert

   Acetamidase
   

   Use

   Tags

   mNeonGreen

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLSPR26

   Plasmid

   #217311

   Purpose

   GAL7(p)-amdS:mRuby3 fusion reporter

   Depositor

   James Fraser

   Article

   Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

   Insert

   Acetamidase
   

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   GAL7

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLSPR27

    Plasmid

    #217312

    Purpose

    TEF1(p)-amdS:mRuby3 fusion reporter

    Depositor

    James Fraser

    Article

    Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

    Insert

    Acetamidase
    

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLSPR28

    Plasmid

    #217313

    Purpose

    GAL7(p)-amdS:mNeonGreen fusion reporter

    Depositor

    James Fraser

    Article

    Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

    Insert

    Acetamidase
    

    Use

    Tags

    mNeonGreen

    Expression

    Yeast

    Mutation

    Promoter

    GAL7

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLSPR29

    Plasmid

    #217314

    Purpose

    TEF1(p)-amdS:mNeonGreen fusion reporter

    Depositor

    James Fraser

    Article

    Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

    Insert

    Acetamidase
    

    Use

    Tags

    mNeonGreen

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRISPR2_IHFdist_rm

    Plasmid

    #162318

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with the IHFdistal binding site removed

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    IHF distal site is removed

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPR2_IRdist_scram

    Plasmid

    #162319

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with a scrambled distal Inverted Repeat of the upstream motif

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The distal Upstream Motif site is scrambled

    Promoter

    pBAD

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Shim_AspRS_TP_GFP_pK7FWG2

    Plasmid

    #202647

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    N-terminal transit peptide from putative cytosolic AspRS
    

    Use

    Tags

    Green Florescent Protien

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. MDM4_CRISPRi_gRNA_2

    Plasmid

    #195133

    Purpose

    gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MDM4 CRISPRi gRNA 2 (MDM4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MDM4_CRISPRi_gRNA_1

    Plasmid

    #195132

    Purpose

    gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MDM4 CRISPRi gRNA 1 (MDM4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRISPR3_BC2

    Plasmid

    #191857

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRISPR3_BC6

    Plasmid

    #191861

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPR3_BC5

    Plasmid

    #191860

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge