We narrowed to 709 results for: pcdna3 1 plasmid

1. (424) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5, K852A)

   Plasmid

   #168191

   Purpose

   HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to an inactive truncated OGT of 4 TPRs with K852A mutation

   Depositor

   Christina Woo

   Article

   Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

   Insert

   HA-nLag6-(EAAAK)4-OGT(4,K852A)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-sPDGFRalpha-Fc

   Plasmid

   #136438

   Purpose

   Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   PDGFRalpha (PDGFRA Human)
   

   Use

   Tags

   Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…

   Expression

   Mammalian

   Mutation

   Extracellular domain of PDGFR-alpha (a.a. 1-524)

   Promoter

   CMV

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-CAG-NanoLuc-VChR1-EYFP

   Plasmid

   #114112

   Purpose

   fusion protein of NanoLuc, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   NanoLuc; Volvox channelrhodopsin 1; EYFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 GIL-11 myc TEV TwinStrep

   Plasmid

   #199627

   Purpose

   Eucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tag

   Depositor

   Jurgen Scheller

   Article

   Rafii et al Commun Biol. 2023 Apr 15;6(1):418. doi:

   Insert

   GIL-11
   

   Use

   Tags

   TwinStrep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-GSDMD_1-270

   Plasmid

   #218229

   Purpose

   Gasdermin1-270 is a pore-forming GSDMD formed by SARS-CoV-2 3CLpro cleavage. Transfection in HEK293 cells showed GSDMD 1-270 pores secrete 3CLpro, nucleocapsid, and in excess, kill cells by pyroptosis

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD (GSDMD Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   construct only codes for amino acids 1-270

   Promoter

   Available since

   July 1, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA3.1-GSDMD_1-275

   Plasmid

   #218230

   Purpose

   Gasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosis

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD (GSDMD Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   construct only codes for amino acids 1-275

   Promoter

   Available since

   July 1, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pcDNA3.1/TO-myc-BirA*-NES

   Plasmid

   #112714

   Purpose

   For tetracycline-inducible expression of myc-BirA*-NES in mammalian cells. For BioID studies.

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

   Insert

   HIV Rev nuclear export signal (NES)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-CXCL12-sfGFP

   Plasmid

   #98961

   Purpose

   Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CXCL12 (CXCL12 Human)
   

   Use

   Tags

   Superfolder GFP

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-hACE2-LgBiT

   Plasmid

   #215837

   Purpose

   Expresses hACE2 receptor with LgBiT tag in mammalian cells

   Depositor

   Thomas Gallagher

   Article

   Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

   Insert

   hACE2-LgBiT (ACE2 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-Flag-Tet1 CD

    Plasmid

    #70129

    Purpose

    Express flag-tagged mouse Tet1 catalytic domain.

    Depositor

    Yi Zhang

    Article

    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

    Insert

    Ten-Eleven Translocation 1 (Tet1 Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    The plasmid expresses TET1 catalytic domain.

    Promoter

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-sPDGFRalpha-Fc (Legacy)

    Plasmid

    #136437

    Purpose

    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    PDGFRalpha (PDGFRA Human)
    

    Use

    Tags

    Fc region of IgG1 (a.a. Pro-217 to Lys-447) and L…

    Expression

    Mammalian

    Mutation

    Extracellular domain of PDGFR-alpha (a.a. 1-524)

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-HCMVgH-8his (Merlin)

    Plasmid

    #136447

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein H (UL75 Human betaherpesvirus 5)
    

    Use

    Tags

    Short GGSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular domain (a.a. 1-716). Codon-optimize…

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3_SARS-CoV-Spike[WIV1 RBD]

    Plasmid

    #190087

    Purpose

    express chimeric SARS-CoV spike with the WIV-1 receptor binding domain

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

    Insert

    SARS-CoV S (S Synthetic)
    

    Use

    Tags

    Rhodopsin C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3_SARS-CoV-Spike[Khosta1 RBD]

    Plasmid

    #190082

    Purpose

    express chimeric SARS-CoV spike with the Khosta-1 receptor binding domain

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

    Insert

    SARS-CoV S (S Synthetic)
    

    Use

    Tags

    Rhodopsin C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry

    Plasmid

    #124429

    Purpose

    Expresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(TEV cleavage seq) T2A mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 NL-BCL2L1

    Plasmid

    #113458

    Purpose

    NanoLuc-BCL2L1 expression vector (positive control together with pcDNA3.1 PA-mCit-BAD)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    BCL2 like 1 (BCL2L1 Human)
    

    Use

    Luciferase

    Tags

    cmyc-NanoLuc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-HCMVgH-8his (TB40/E)

    Plasmid

    #136445

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein H (UL75 Human betaherpesvirus 5)
    

    Use

    Tags

    Short GGSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular domain (a.a. 1-717). Codon-optimize…

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-N-Myc_DED/AAA 535-888 USP7

    Plasmid

    #131255

    Purpose

    Mammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    A mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-sPDGFRalpha-Fc-Y206S

    Plasmid

    #136439

    Purpose

    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (Y206S mutant)

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    PDGFRalpha (PDGFRA Human)
    

    Use

    Tags

    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…

    Expression

    Mammalian

    Mutation

    Extracellular domain of PDGFR-alpha (a.a. 1-524) …

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-sPDGFRalpha-Fc-V242K

    Plasmid

    #136440

    Purpose

    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (V242K mutant)

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    PDGFRalpha (PDGFRA Human)
    

    Use

    Tags

    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…

    Expression

    Mammalian

    Mutation

    Extracellular domain of PDGFR-alpha (a.a. 1-524) …

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge